Microscopy

Question 1

What do light microscopes have?

Answer 1

2 lenses- objective and eye piece
Usually illuminated from underneath
Maximisation of x2000
Resolution power of 200nm
Used to see tissues, cells and large sub-cellular structures

Question 2

What does electron microscopes have?

Answer 2

Deep inside sub cellular structures
Electrons are used to form an image
2 types: scanning electron microscope creates 3D images, Transmission electron microscope creates 2D images
Magnification of up to x2000000 and a resolving power of 10nm and 0.2nm

Question 3

Magnification of a light microscope

Answer 3

Magnification of the eyepiece lens x magnification of the objective lens

Question 4

How to find size of an object?

Answer 4

Size of image/ magnification