Microscopy Flashcards
1
Q
Define magnification:
A
The number of times an image is enlarged, compared to the actual size of the object
2
Q
Define resolution:
A
The ability to distinguish between two points clearly as separate.
3
Q
What determines resolution?
A
Shorter wavelength = high resolution power
Max resolution = half wavelength used
4
Q
Features of a light microscope:
A
Wavelength = 400-700nm
Lower energy and longer wavelength
Highest resolution= x1500
Max resolution =200nm
5
Q
Features of an electron microscope:
A
Wavelength = 1nm
High energy and short wavelength
Vacuum
Views dead specimens
Max resolution = 0.5 nm
Max magnification =x250000
