Microscopy

Question 1

-Stained Tissue is examined with ordinary
light passing through the preparation.
- The microscope includes an optical system
and mechanism to move and focus the specimen.

Answer 1

Bright-Field Microscope

Question 2

• Typically used for study of brightfield
  microscopic preparations.
• Involves the conversion of a stained tissue
  preparation to high-resolution digital images and
  permits study of tissue using a computer or other
  digital device, without an actual stained slide or a
  microscope.

Answer 2

Virtual Microscopy
(can be zoomed in and out)

Question 3

• Tissue sections are usually irradiated with
  ultraviolet (UV) light and the emission is in the visible
  portion of the spectrum.
• The fluorescent substances appear bright on
  a dark background.

Answer 3

Fluorescence Microscopy

Question 4

• Uses a lens system that produces visible
  images from transparent objects and can be used
  with living, cultured cells. Unstained cells and tissue
  sections, which are usually transparent and colorless,
  can be studied with these modified light microscope.

Answer 4

Phase-Contrast Microscopy

Question 5

• A modification of phase-contrast microscopy
  with Nomarski optics, which produces an image of
  living cells with a more apparent three-dimensional
  (3D) aspect.

Answer 5

Different Interference Microscopy

Question 6

• Achieves high resolution and sharp focus by
  using:
  (1) A small point of high intensity light, often
  from a laser.
  (2) A plate with a pinhole aperture in front of
  the image detector.

Answer 6

Confocal Microscopy

Question 7

Where the light beam passes through in Confocal Microscopy

Answer 7

aperture

Question 8

• Allows the recognition of stained or
  unstained structure made of highly organized sub
  units.
• They appear as bright structures against a
  dark background.

Answer 8

Polarizing Microscopy
(only collagen fibers are visible)

Question 9

are based on the interaction of tissue components with
beams of electrons. The wavelength in an electron
beam is much shorter than that of light, allowing a
1000-fold increase in resolution

Answer 9

Transmission and Scanning electron microscope

TEM and SEM

Question 10

• Basic principles of operation: A beam of
  electrons focused using electromagnetic “lenses”
  passes through the tissue section to produce an
  image with black, white, and intermediate shades of
  gray regions.

Answer 10

Transmission Electron Microscope (TEM)
can increase as much as 400x

Question 11

• Provides a high resolution view of the
  surfaces of cells, tissues, and organs.
• Like the TEM, this microscope produces and
  focuses a very narrow beam of electrons, but in
  instrument the beam does not pass through the
  specimen.

Answer 11

Scanning Electron Microscope (SEM)

Question 12

3D and provides surface view, without cutting unlike TEM

Answer 12

Scanning Electron Microscope (SEM)

Question 13

• Magnifies the primary image formed by the
  objective lens.

Answer 13

Eyepiece (ocular lens)

Question 14

• Designed to hold objective lenses
  permitting changes of magnification by rotating
  different powered objective lenses into optical path.

Answer 14

Nosepiece (revolving turret)

Question 15

• Main support for microscope components.

Answer 15

Arm

Question 16

• Two locked-on clips hold specimen slide in
  place on stage.

Answer 16

Stage Clips

Question 17

• Large knobs located on each side of arm,
  raise or lower stage to bring specimen image into
  focus.

Answer 17

Coarse Focusing Knobs

Question 18

• Smaller knobs, located close to the coarse
  focusing knobs, permit more precise adjustment of
  the image.

Answer 18

Fine Focusing Knobs

Question 19

• Allows the user to move the side in the X or
  Y axis direction.

Answer 19

Mechanical Stage Adjustment Knob

Question 20

Housing and platform of the instrument to
which the arm is attached in addition it usually
contains an illumination system for the microscope.

Answer 20

Base

Question 21

• Provides adjustable current voltage for the
  light source.
• The intensity of the light is adjusted by
  turning this knob.

Answer 21

Voltage Regulator

Question 22

The light source for a microscope.

Answer 22

Illumination

Question 23

• It is designed to help achieve optimum
  resolution of the objective lens.
  -opening and closing is controlled by lever, controls light going into condenser

Answer 23

Diaphragm

Question 24

• Condenses light rays from sub stage
  illumination and fills the back element of objective
  lens to improve image resolution.

Answer 24

Condenser

Question 25

• Platform of the microscope where the
  specimen slide is placed, it permits precise,
  mechanical manipulation of the specimen slide.

Answer 25

Mechanical Stage

Question 26

• Lens closest to the object being viewed,
  forms first image of the specimen.

Answer 26

Objective Lens

Question 27

• Adjustable eyepiece diopter permits
  focusing adjustment of image for any difference in
  vision between users eyes.

Answer 27

Doipter Adjustment

Question 28

• Daylight blue filter designed to make
  incandescent illumination appear white.

Answer 28

Filter

Question 29

• The switch that turns the illuminator on and
  off.

Answer 29

Power Switch

Question 30

Types of Objective Lenses

Answer 30

• Scanner - 4x
• Low Power Objective (LPO) - 10x
• High Power Objective (HPO) - 40x
• Oil Immersion Objective (OIO) - 100x

Question 31

Power of Eyepiece lens

Answer 31

10x

Question 32

Total Power of Objective lenses is = to

Answer 32

Power of Eyepiece lens * Power of Objective lens

Question 33

TRUE OR FALSE

It is important to lift a microscope with the use of
two hands.

Answer 33

TRUE

Question 34

TRUE OR FALSE

Ocular and Objective lenses must be cleansed with ONLY lens paper and dampened with a lens cleaning solution

Answer 34

TRUE

Question 35

TRUE OR FALSE

use disinfectant (70% Isopropyl alcohol) to
clean the stage before returning them to the
stock room.

Answer 35

TRUE