Intro to HISTO

Question 1

is the study of the tissues of the body
and
how these tissues are arranged to constitute organs.

Answer 1

HISTOLOGY

Question 2

Study of the tissue in order to study the
manifestation of the disease

Answer 2

HISTOPATHOLOGY

Question 3

field of science that involves processing of tissue
to be able to study them under the microscope

Answer 3

HISTOTECHNOLOGY

Question 4

Tissues have two interacting components:

Answer 4

Cells
and Extracellular matrix or ECM

Question 5

supports the cells and contains the
fluid transporting nutrients to the cells, and
carrying away their wastes and secretory
products.

Answer 5

ECM

Question 6

The most common procedure used in histologic
research

Answer 6

the preparation of tissue slices or “sections” that can be examined visually with
transmitted light.

Question 7

are thin, flat slices of fixed
and stained tissues or organs mounted on glass
slides.

Answer 7

Histologic Sections

Question 8

The correct visualization and interpretation of
sections in their proper three-dimensional
perspective on the slide.

Answer 8

Histologic Sections

Question 9

plane of section that cuts in the
midline.

Answer 9

Longitudinal

Question 10

a plane that intersects the
longitudinal axis at right angle.

Answer 10

Transverse

Question 11

a plane that produces a round structure

Answer 11

Transverse

Question 12

graze or only pass through the
outermost periphery.

Answer 12

Tangential

Question 13

a plane that is neither longitudinal or transverse

Answer 13

Oblique

Question 14

A plane that produces an oval structure

Answer 14

Oblique

Question 15

techniques used to preserve via (37% formaldehyde) which inhibits autolysis through its own enzymes, degrades biological molecules

Answer 15

Histotechniques

Question 16

used in biopsies

Answer 16

Histotechniques

Question 17

Small pieces of tissue are placed in
solutions of chemicals that cross-link proteins
and inactive degradative enzymes, which
preserves cell and tissue structure.

Answer 17

Fixation

Question 18

The tissue is transferred through a
series of increasingly concentrated alcohol
solutions, ending in 100% , which removes all
water.

Answer 18

Dehydration

Question 19

Alcohol is removed in organic solvents
in which both alcohol and paraffin are miscible.

Answer 19

Clearing

Question 20

gives a translucent appearance

Answer 20

Clearing

Question 21

The tissue is then placed in melted
paraffin until it becomes completely infiltrated
with this substance.

Answer 21

Infiltration

Question 22

put into an oven (52-60 Celsius) to evaporate clearing solvent

Answer 22

Infiltration

Question 23

The paraffin-infiltrated tissue is
placed in a small mold with melted paraffin and
allowed to harden at room temp.

Answer 23

Embedding

Question 24

The resulting paraffin block is trimmed
to expose the tissue for sectioning (slicing) on a
microtome.

Answer 24

Trimming

Question 25

Uses a device called microtome and
can cut tissues into sections 1 to 10 micrometers
thick.

Answer 25

Sectioning

Question 26

Thin sections are mounted upon glass
slides.

Answer 26

Mounting

Question 27

Process that permit distinctions of
tissue components.

Answer 27

Staining

Question 28

a BASIC dye that stains ACIDIC parts BLUE, stains the NUCLEUS and ROUGH ER

Answer 28

Hematoxin

Question 29

an ACIDIC dye that stains BASIC parts PINK, stains the CYTOPLASM, MITOCHONDRIA and LYSOSOMES

Answer 29

Eosin

Question 30

• Nuclei stain black or blue black.
• Muscles stain red.
• Collagen and Mucus stain green or blue.
• Cytoplasm of most cells stains pink.

Answer 30

Masson’s Trichrome Stain

Question 31

• Glycogen stains deep red or magenta.

Answer 31

Period Acidic-Schiff Reaction (PAS)

Question 32

• Elastic fiber stain jet black.
• Nuclei stain gray.
• Remaining structures stain pink.

Answer 32

Verhoeff’s Stain for Elastic Tissue

Question 33

• Erythrocytes stain red-orange.
• Cytoplasm of liver and kidney stains pink.
• Nuclei stain red.
• Fibrous connective tissue, mucus, and
  hyaline cartilage stain deep blue.

Answer 33

Mallory-Azan Stain

Question 34

Mature RBC’s

Answer 34

Erythrocytes

Question 35

• Erythrocyte cytoplasm stains pink.
• Lymphocyte nuclei stain dark purple-blue
  with pale blue cytoplasm.
• Neutrophil nuclei stain dark blue
• Eosinophil nuclei stain dark blue and the
  granules stain bright pink.
• Monocyte cytoplasm stains pale blue and
  nucleus stains medium blue.

Answer 35

Wright’s or Giemsa’s Stain

Question 36

Stain used in Blood Smears

Answer 36

Wright’s or Giemsa’s Stain

Question 37

• Myelinated and Unmyelinated fibers and
  neurofibrils stain blue-black.
• General background is nearly colorless.
• Astrocytes stain black.
• Depending on the methods used, the end
  product can stain black, brown, or gold.

Answer 37

Cajal’s and Del Rio Hortega’s Methods (Silver and
Gold Methods)

Question 38

• Lipids in general stain black.
• Lipids in myelin sheath of nerves stain black.

Answer 38

Osmic Acid (Osmium Tetroxide) Stain

Question 39

Reagent Material in Fixation

Answer 39

37% Formaldehyde

Question 40

Reagent Material in Dehydration

Answer 40

Ethanol

Question 41

Reagent Material in Clearing

Answer 41

Xylene

Question 42

Reagent Material in Infiltration and Embedding

Answer 42

Paraffin Wax

Question 43

Reagent Material in Sectioning

Answer 43

Glycol methacrylate

Question 44

Reagent Material in Mounting

Answer 44

Organic Solvent Based mounting media.
These are natural or synthetic resins
dissolved in benzene, toluene or xylene and
are used when a permanent mount is
required and frequently used in routine H
and E staining procedures.

Question 45

Reagent Material in Staining

Answer 45

Haematoxylin and eosin (H & E): Routine
stain

Question 46

These are natural or synthetic resins
dissolved in benzene, toluene or xylene and
are used when a permanent mount is
required and frequently used in routine H
and E staining procedures.

Answer 46

Organic Solvent Based mounting media.