MICROSCOPY Flashcards
Is the science of magnification and resolution.
Microscopy
Factors affecting Resolution
Wavelength of Light used
Numerical Aperture
MAGNIFYING GLASS WITH 6X-10X MAGNIFYING POWER
EARLY “MICROSCOPE”
In 1590 They put several lenses in a tube and made a very important discovery
Zaccharias Janssen and his father
One of the pioneers of microscopy who in the late 17th century became the first man to make and use a real microscope.
Anton Van Leeuwenhoek
electrons bounce off the surface of the specimen resulting in a more 3D image
Scanning
electrons transmitted through substance
Transmission
is defined as a change in direction of a wave at an interface between 2 different media so that the waveform returns to the media from which it came
Reflection
Change in the direction of a wave (light) due to a change in speed
Refraction
a measure of the speed of light in material
the ratio of the velocity of light in a vacuum to the speed of light in the specified material
Used in calculating focusing power of lenses and dispersion properties of prisms
Refractive Index
objective is a measure of its ability to gather light
Numerical Aperture
At least 3 Types of Microscopy
Simple Microscope Electron Microscope Bright-field Fluorescence Confocal Dissecting Microscope
are light illuminated. The imageseen with this type of microscope is two dimensional.
Compound Light
Uses phase shifted waves of through transparent specimens cause changes in amplitude (contrast) in structures of the specimen. One of the most widely used in biology
Phase Contrast
utilizes fluorescent dyes/stains that fluoresce when radiated with specific wavelengths of light
Fluorescence
