MICROSCOPIC EXAMINATION Flashcards
Urine specimen preparation should use what specimen
Fresh/adequately preserved
Iced specimen shall undergo
Warmth @RT
Refrigeration of urine will cause
Precipitation of amorphous urates/phosphates
Warming at 37 temp will cause
Crystals to disintegrate
Standard amount of urine collected in a container
10-15 ml
Common amount of urine collected
12 ml
Centrifiguration under
5 minutes @400 RCF
Sediment preparation uses
0.5 mL and 1 mL
Conventional glass slide method uses
0.02 mL or 20 uL
Examining sediment: LPO
Cast
General composition of sediment
Examining sediment: HPO
Identification of Sediment
Reporting of ME: Cast
Per LPF
Reporting of ME: RBC & WBC
Per 10 HPF
Most commonly used stain
Stainhaimer malbin
Sternhaimer malbin contains
Crystal violet and Safranin O
Commercially prepared Sterhaimer
Kova stain, Sedi stain
Stain used for TGL, CHOLE, Lipids
Lipid stain
Oil Red O
Sudan III
Polarizing Microscope
Stain used for bacterial cast
VIAS
Bacterial cast usually mistaken as
Granular cast
Stain used for Eosinophil
Hansel stain
Hansel stain is used under what circumstances
Drug-induced allergic reaction causing inflammation in renal interstitium
Hansel stain contains
Methylene blue and Eosin Y
Stain used for Iron
Prussian Blue
what is being stained by Prussian Blue
Hemosiderin granules