MICROSCOPIC EXAMINATION Flashcards

1
Q

Urine specimen preparation should use what specimen

A

Fresh/adequately preserved

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2
Q

Iced specimen shall undergo

A

Warmth @RT

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3
Q

Refrigeration of urine will cause

A

Precipitation of amorphous urates/phosphates

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4
Q

Warming at 37 temp will cause

A

Crystals to disintegrate

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5
Q

Standard amount of urine collected in a container

A

10-15 ml

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6
Q

Common amount of urine collected

A

12 ml

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7
Q

Centrifiguration under

A

5 minutes @400 RCF

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8
Q

Sediment preparation uses

A

0.5 mL and 1 mL

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9
Q

Conventional glass slide method uses

A

0.02 mL or 20 uL

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10
Q

Examining sediment: LPO

A

Cast
General composition of sediment

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11
Q

Examining sediment: HPO

A

Identification of Sediment

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12
Q

Reporting of ME: Cast

A

Per LPF

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13
Q

Reporting of ME: RBC & WBC

A

Per 10 HPF

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14
Q

Most commonly used stain

A

Stainhaimer malbin

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15
Q

Sternhaimer malbin contains

A

Crystal violet and Safranin O

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16
Q

Commercially prepared Sterhaimer

A

Kova stain, Sedi stain

17
Q

Stain used for TGL, CHOLE, Lipids

A

Lipid stain
Oil Red O
Sudan III
Polarizing Microscope

18
Q

Stain used for bacterial cast

A

VIAS

19
Q

Bacterial cast usually mistaken as

A

Granular cast

20
Q

Stain used for Eosinophil

A

Hansel stain

21
Q

Hansel stain is used under what circumstances

A

Drug-induced allergic reaction causing inflammation in renal interstitium

22
Q

Hansel stain contains

A

Methylene blue and Eosin Y

23
Q

Stain used for Iron

A

Prussian Blue

24
Q

what is being stained by Prussian Blue

A

Hemosiderin granules

25
Q

Type of Microscope depends

A

Type of specimen
Refractive index of object
Ability to image unstained living cells

26
Q

Object appears light against dark bg

A

Dark field microscopy

27
Q

Object appears DARK against LIGHT BG

A

Bright-field Microscopy

28
Q

Enhances visualization in low refractive indices

A

PHASE-CONTRAST

29
Q

Sediments detectable in Phase contrast

A

Hyaline cast
Mucous threads
Mixed cellular cast
Trichomonas

30
Q

Provides three-dimensional image

A

Interference-contrast

31
Q

Independent in routine urinalysis

A

Cytodiagnostic urine testing

32
Q

Significance of Cytodiagnostic urine testing

A

Detect malignancy in lower urinary tract

33
Q

Centrifuge used in Cytodiagnostic UT

A

Cytocentrifiguration

34
Q

Stain used for Cytodiagnostic test

A

Papanicolaus stain