MICROSCOPIC EXAMINATION Flashcards

1
Q

Urine specimen preparation should use what specimen

A

Fresh/adequately preserved

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2
Q

Iced specimen shall undergo

A

Warmth @RT

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3
Q

Refrigeration of urine will cause

A

Precipitation of amorphous urates/phosphates

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4
Q

Warming at 37 temp will cause

A

Crystals to disintegrate

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5
Q

Standard amount of urine collected in a container

A

10-15 ml

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6
Q

Common amount of urine collected

A

12 ml

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7
Q

Centrifiguration under

A

5 minutes @400 RCF

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8
Q

Sediment preparation uses

A

0.5 mL and 1 mL

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9
Q

Conventional glass slide method uses

A

0.02 mL or 20 uL

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10
Q

Examining sediment: LPO

A

Cast
General composition of sediment

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11
Q

Examining sediment: HPO

A

Identification of Sediment

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12
Q

Reporting of ME: Cast

A

Per LPF

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13
Q

Reporting of ME: RBC & WBC

A

Per 10 HPF

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14
Q

Most commonly used stain

A

Stainhaimer malbin

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15
Q

Sternhaimer malbin contains

A

Crystal violet and Safranin O

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16
Q

Commercially prepared Sterhaimer

A

Kova stain, Sedi stain

17
Q

Stain used for TGL, CHOLE, Lipids

A

Lipid stain
Oil Red O
Sudan III
Polarizing Microscope

18
Q

Stain used for bacterial cast

19
Q

Bacterial cast usually mistaken as

A

Granular cast

20
Q

Stain used for Eosinophil

A

Hansel stain

21
Q

Hansel stain is used under what circumstances

A

Drug-induced allergic reaction causing inflammation in renal interstitium

22
Q

Hansel stain contains

A

Methylene blue and Eosin Y

23
Q

Stain used for Iron

A

Prussian Blue

24
Q

what is being stained by Prussian Blue

A

Hemosiderin granules

25
Type of Microscope depends
Type of specimen Refractive index of object Ability to image unstained living cells
26
Object appears light against dark bg
Dark field microscopy
27
Object appears DARK against LIGHT BG
Bright-field Microscopy
28
Enhances visualization in low refractive indices
PHASE-CONTRAST
29
Sediments detectable in Phase contrast
Hyaline cast Mucous threads Mixed cellular cast Trichomonas
30
Provides three-dimensional image
Interference-contrast
31
Independent in routine urinalysis
Cytodiagnostic urine testing
32
Significance of Cytodiagnostic urine testing
Detect malignancy in lower urinary tract
33
Centrifuge used in Cytodiagnostic UT
Cytocentrifiguration
34
Stain used for Cytodiagnostic test
Papanicolaus stain