MICROSCOPIC EXAMINATION Flashcards
Urine specimen preparation should use what specimen
Fresh/adequately preserved
Iced specimen shall undergo
Warmth @RT
Refrigeration of urine will cause
Precipitation of amorphous urates/phosphates
Warming at 37 temp will cause
Crystals to disintegrate
Standard amount of urine collected in a container
10-15 ml
Common amount of urine collected
12 ml
Centrifiguration under
5 minutes @400 RCF
Sediment preparation uses
0.5 mL and 1 mL
Conventional glass slide method uses
0.02 mL or 20 uL
Examining sediment: LPO
Cast
General composition of sediment
Examining sediment: HPO
Identification of Sediment
Reporting of ME: Cast
Per LPF
Reporting of ME: RBC & WBC
Per 10 HPF
Most commonly used stain
Stainhaimer malbin
Sternhaimer malbin contains
Crystal violet and Safranin O