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AS OCR Biology > Microscopes (from powerpoint) > Flashcards

Microscopes (from powerpoint) Flashcards

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AP® Biology flashcards Biology 101 flashcards
1
Q

What is the purpose of staining?

A

Stains make stuctures visible/ increase contrast.

So you can see different organelles/ recognise different types of cells.

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2
Q

What are the 2 types of stains?

A

General stains and differentiated stains.

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3
Q

What are differentiated stains?

A

Stains that bind to specific cell structures, staining each differently so they can be easily identified within a single preparation.

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4
Q

What is methylene blue?

A

An all purpose stain (blue)

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5
Q

What is Sudan red?

A 
Differentiated stain
Lipid stain (red)
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6
Q

What is iodine?

A

Differentiated stain
Starch-blue/black
Cellulose- yellow

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7
Q

What is magnification?

A

How many times bigger the image is compared to the actual size.

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8
Q

What is resolution?

A

The minimum distance apart 2 objects must be in order for them to appear as seperate items.
(better resolution = clearer image)

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9
Q

What is the type of beam in a light microscope?

A

Light

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10
Q

What is the type of beam in a laser scaning (confocal) light microscope?

A

Laser light

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11
Q

What is the type of beam in an electron scanning microscope?

A

Electron

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12
Q

What is the type of beam in an electron transmission light microscope?

A

Electron

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13
Q

What is the wavelength of a beam in a light microscope?

A

400-700nm

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14
Q

What is the wavelength of a beam in a laser scanning (confocal) microscope?

A

400-700nm

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15
Q

What is the wavelength of a beam in an electron scanning microscope?

A

0.004nm

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16
Q

What is the wavelength of a beam in an electron transmission microscope?

A

0.004nm

17
Q

What is the magnification in a light microscope?

A

x1500-x2000

18
Q

What is the magnification in a laser scanning (confocal) microscope?

A

x1500 - x2000

19
Q

What is the magnification is a electron scanning microscope?

A

x100,000 - x500,000

20
Q

What is the magnification in a transmission electron microscope?

A

x500,000 - x2,000,000

21
Q

What is the resolution of a light microscope?

A

200nm

22
Q

What is the resolution of a laser scanning (confocal) microscope?

A

800nm

23
Q

What is the resolution of an electron scanning microscope?

A

3-10nm

24
Q

What is the resolution of an electron transmission microscope?

A

0.2-0.5nm

25
Q

How are specimens prepared for a light microscope?

A

Place on glass slide

26
Q

How are specimens prepared for a laser scanning (confocal) microscope?

A

Specimen can be tagged with fluorescent dye

27
Q

How are specimens prepared for a electron scanning microscope?

A

Speciemen coated with fine layer of metal

28
Q

How are speciens prepared for a electron transmission microscope?

A

Thinly spiced, chemically fixed by dehydration and stained.

29
Q

What image is produced from a light microscope?

A

2D colour image

30
Q

What image is produced by a laser scanning (confocal) microscope?

A

3D image

31
Q

What image is produced by a electron scanning microscope?

A

3D image with false colour

32
Q

What image is produced by a electron transmission microscope?

A

2D grey scale image

33
Q

Can a light microscope be used to view living organisms?

A

Yes

34
Q

Can a laser scanning (confocal) microscope be used to view living organisims?

A

Yes

35
Q

Can an electron scanning microscope be used to view living organisms?

A

No

36
Q

Can an electron transmission microscope be used to view living organisms?

A

No

37
Q

What is the special viewing condition for electron microscopes?

A

Viewed in a vacuum, stained with metal salts.

AS OCR Biology (14 decks)

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