Microscopes and magnification Flashcards
Light microscope
Uses light to magnify objects. The greatest possible magnification using a light microscope is about 2000 x
Electron microscope
Uses electrons to view an object. This makes it possible to magnify objects up to about 10 million x. You can see objects in cells more clearly and in far more detail with an electron microscope
Calculating magnification
Magnification = Image Size/Real Size
How to measure magnification
Measure image in mm
Multiply by 1000 for um (micrometers)
Millimeter
10^-3 meters
Micrometer
10^-6 meters
Nanometer
10^-9 meters
Pico meters
10^-12 meters
How to use a light microscope
Start with the lowest power objective under the eyepiece
Club the slide securely on the stage
Adjust the light source so that the light goes through the slide
Use the focusing wheel to focus on the slide
Move the slide so that the cell you will draw is in the middle of the view
If needed, move a higher power objective into position
Use the fine focusing wheel to bring the cell back into focus