Microscopes Flashcards
What is the wavelength of light?
400-700nm
What is the wavelength of a TEM and SEM?
0.005nm
Describe the specimen in a light microscope
Living / non-living on slide
Describe the specimen in a TEM
Non-living
Copper grid
Vacuum
Describe the specimen in a SEM
Non-living
Metal disc
Vacuum
What is the maximum resolution of a light microscope?
200nm
What is the maximum resolution of a TEM?
0.5nm
What is the maximum resolution of a SEM?
3-10nm
What is the maximum magnification of a light microscope?
X 1500
What is the maximum magnification of a TEM?
At least X 500,000
What is the maximum magnification of a SEM?
X 100,000
What is the type of image displayed from a light microscope?
2D colour
What is the type of image displayed from a TEM?
2D black and white
What is the type of image displayed from a SEM?
3D black and white
Colour can be added on a computer
Stains for a light microscope
Coloured dyes
Stains for TEM
Heavy metals
Stains for SEM
Carbon or gold