Microscopes Flashcards
1
Q
The electron microscope
A
The electron microscope is a type of microscope that uses a beam of electrons to create an image of the specimen. It is capable of much higher magnifications and has a greater resolving power than a light microscope, allowing it to see much smaller objects in finer detail.
2
Q
what are the limitations of using an electron microscope?
A
Specimen preparation is crucial and you can’t view live organisms as it has to be in a vacuum
3
Q
What are the common staining agents of TEM?
A
Uranium and Lead
4
Q
What does SEM produce a picture of?
A
Cell surface
5
Q
What is FRAP used for?
A
determine the kinetics of protein redistribution/protein trafficking and to detect how much protein is immobile and how much is mobile
6
Q
What are the disadvantages of using GFP?
A
Photobleaching Cellular distribution Protein folding Over expression Phytotoxicity
