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Biology-Cell Structure > Microscope Types > Flashcards

Microscope Types Flashcards

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1
Q

When were Light Microscopes developed?

A

1600’s

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2
Q

How is Light Focussed?

A

Using Glass lenses

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3
Q

What value can it magnify objects up to?

A

x 1500

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4
Q

What is the Resolving Power

A

about 0.2 μm

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5
Q

Magnification

A

Magnification is how many times bigger the image of a specimen observed is in compared to the actual (real-life) size of the specimen.

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6
Q

Resolution

A

Resolution is the ability to distinguish between two separate points.

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7
Q

What are the advantages of using Light Microscopes?

A

-Cheap and can be used anywhere.-Easy to use and easy to prepare specimens.-Can observe anything, dead or live.

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8
Q

What are the disadvantages of using Light Microscopes?

A

-Limited Magnification and Resolution.
-May need to use a stain to see organelles.

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9
Q

When were Electron Microscopes developed?

A

1930’s

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10
Q

What is the magnification and resolution like?

A

Relatively Low.

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11
Q

What can it not view?

A

It is in-satisfactory for viewing very small organelles.

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12
Q

What are the advantages of Electron Microscopes?

A

-Can see inside organelles.

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13
Q

What are the disadvantages of Electron Microscopes?

A

-Large and Expensive-Difficult to use/prepare specimens -Have to be kept in temperature/pressure and humidity controlled rooms.-Can produce artefacts-Cannot view live specimens.
-Only produce B&W images

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14
Q

What is a TEM?

A

Transmission Electron Microscope

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15
Q

What Images do TEM’s produce?

A

2D

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16
Q

What Magnification do TEM’s have?

A

High Magnification > x1 000 000

17
Q

What Resolution do TEM’s have?

A

High Resolution(0.05 - 2 nm)

18
Q

In what detail can TEM’s see in?

A

Have the ability to see inside organelles.

19
Q

What is an SEM?

A

Scanning Electron Microscope

20
Q

What type of Images do SEM’s produce?

A

3D Images

21
Q

What Magnification do SEM’s have?

A

Low Magnification< x 500 000

22
Q

What Resolution do SEM’s have?

A

Low Resolution(5-50 nm)

23
Q

In what detail can SEM’s see in?

A

Only has the ability to see outer surface.

24
Q

What is the equation for Magnification?

A

Magnification=Image Size/Actual Size

Biology-Cell Structure (7 decks)

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