Microscope Types Flashcards
When were Light Microscopes developed?
1600’s
How is Light Focussed?
Using Glass lenses
What value can it magnify objects up to?
x 1500
What is the Resolving Power
about 0.2 μm
Magnification
Magnification is how many times bigger the image of a specimen observed is in compared to the actual (real-life) size of the specimen.
Resolution
Resolution is the ability to distinguish between two separate points.
What are the advantages of using Light Microscopes?
-Cheap and can be used anywhere.-Easy to use and easy to prepare specimens.-Can observe anything, dead or live.
What are the disadvantages of using Light Microscopes?
-Limited Magnification and Resolution.
-May need to use a stain to see organelles.
When were Electron Microscopes developed?
1930’s
What is the magnification and resolution like?
Relatively Low.
What can it not view?
It is in-satisfactory for viewing very small organelles.
What are the advantages of Electron Microscopes?
-Can see inside organelles.
What are the disadvantages of Electron Microscopes?
-Large and Expensive-Difficult to use/prepare specimens -Have to be kept in temperature/pressure and humidity controlled rooms.-Can produce artefacts-Cannot view live specimens.
-Only produce B&W images
What is a TEM?
Transmission Electron Microscope
What Images do TEM’s produce?
2D
What Magnification do TEM’s have?
High Magnification > x1 000 000
What Resolution do TEM’s have?
High Resolution(0.05 - 2 nm)
In what detail can TEM’s see in?
Have the ability to see inside organelles.
What is an SEM?
Scanning Electron Microscope
What type of Images do SEM’s produce?
3D Images
What Magnification do SEM’s have?
Low Magnification< x 500 000
What Resolution do SEM’s have?
Low Resolution(5-50 nm)
In what detail can SEM’s see in?
Only has the ability to see outer surface.
What is the equation for Magnification?
Magnification=Image Size/Actual Size
