Microscope And Cell Division Tet Flashcards
What is the visible area seen when looking through a microscope?
Field of View decreases as magnification increases.
The field of view refers to how much of the specimen you can see at one time.
What does Depth of Field refer to?
The thickness of the specimen that remains in focus at one time. Thinner at higher magnifications.
A higher magnification leads to a shallower depth of field.
What is Parfocal in microscopy?
A feature where the image stays mostly in focus when switching between different objective lenses.
This allows for easier viewing when changing magnifications.
What is Inversion in microscopy?
The image appears flipped both upside down and left-to-right due to the lens system.
This is a common characteristic of optical systems.
Who invented the microscope?
Anton von Leeuwenhoek.
He was the first to observe and describe single-celled organisms.
Why is it called a Compound Light Microscope?
Compound: it has two lens systems (eyepiece + objective lens); Light: uses visible light to illuminate the specimen.
This type of microscope is distinct from simpler types that use only one lens.
How do you carry a microscope?
Use two hands: one on the arm and one under the base.
Always handle with care as microscopes are expensive and delicate.
What is the function of the Eyepiece (Ocular Lens)?
Lens you look through, typically 10x magnification.
This is the part of the microscope where the viewer looks to see the magnified image.
What does the Revolving Nosepiece (Turret) do?
Holds and rotates the objective lenses.
This allows the user to switch between different magnification levels easily.
What are Objective Lenses?
Primary magnification lenses, commonly 4x, 10x, 40x.
Different objective lenses provide various levels of magnification.
What is the purpose of the Stage & Specimen Holder?
Flat surface that supports the slide; holder secures it in place.
This is essential for stabilizing the specimen during observation.
What is the Coarse Adjustment Knob used for?
Moves stage up/down for general focus (used on low power).
It is primarily used to bring the specimen into general focus.
What does the Fine Adjustment Knob do?
Fine-tunes the focus at any power.
This knob allows for precise focusing once the specimen is generally focused.
What is the function of the Arm in a microscope?
Supports the microscope head; used for carrying.
It provides stability and balance to the microscope.
What is the Iris Diaphragm (Diaphragm)?
Adjusts the amount of light reaching the slide.
Proper lighting is crucial for clear observation of the specimen.
What is the relationship between objective length and magnification?
Shorter Objective → Lower Magnification; Longer Objective → Higher Magnification.
This affects how much detail is visible in the specimen.
If the field of view is 4 mm and an object takes up 1/2 the space, what is the object’s size?
About 2 mm long.
Estimating object size based on field of view is an important skill in microscopy.
What is the formula for Total Magnification?
Eyepiece Magnification × Objective Lens Magnification.
Example: 10x eyepiece × 40x objective = 400x total magnification.
What is a Dissecting Scope also called?
Stereoptic or Binocular.
This type of microscope is designed for viewing larger or thicker specimens.
What are the key features of a Dissecting Scope?
Provides a 3D image and has lower magnification than compound microscopes.
It is often used for examining objects like bugs, rocks, and plants.
What are prokaryotes?
Cells without a nucleus or membrane-bound organelles
Example: Bacteria
How do prokaryotes reproduce?
By binary fission (not mitosis)
What are eukaryotes?
Cells with a nucleus and membrane-bound organelles
