Microscope Flashcards
What helps the intensity between objects or object and background?
Contrast
What distinguish objects that are close together?
Resolution
What helps determine resolution?
Contrast
What increases contrast?
Staining and light in phase
List other portions of microscope
Wavelength of radiation
Magnification
Light
Radiation Scale (low to high)
Purple 400 nm Gamma rays —> Red 700 nm Radio waves/TV
What kind of microscope has simple and compound?
Bright-field
What type of Bright field has single lens?
similar to magnifying glass
Bright field Simple
What Bright Field microscope has a series of lenses?
Light passes through specimen into objective lens
One or two ocular lenses
Bright field Compound
What increases resolution in compound bright field?
Oil Immersion
most have a condenser lens (direct light)
Magnification
Total mag= mag of objective lens X mag of ocular lens
What kind of microscope is best for pale objects?
Dark-field
Describe dark-field
- Light Rays scattered enter objective
- Specimen appears light against dark background
- Increases contrast/enables observations for details
What kind of microscope can be divided into three different microscope?
Light Microscopy
What kind of microscope:
- Examine living organisms that would be damaged if put on slide
- Light rays IN phase–brighter image
- Types: Phase contrast/differential interference contrast
Phase Microscope (light microscopy)
What kind of microscope:
- Direct UV light
- Radiates energy back, visible wavelength
- UV light increases resolution/contrast
- Staining
Fluorescent Microscope (Light Microscopy)
What kind of microscope:
- Fluorescent dyes
- UV lasers to illuminate fluorescent chemicals, single plane
- Resolution increase light passes pinhole
- Computer image
Confocal Microscope (Light Microscopy)
What kind of microscope has a greater resolving power?
10,000 X to 100,000 X magnifies
Electron Microscopy
What can you view with an electron microscopy?
Bacteria, viruses, internal cellular structures, molecules, atoms
What types of electron microscopy are there?
Transmission electron
Scanning electron
List the parts to a Microscope
- Ocular lens
- Body
- Arm
- Objective lens
- Stage
- Condenser
- Diaphragm
- Illuminator
- Coarse focusing knob
- Fine focusing knob
- Base
Part of microscope: re-magnifies the image
Ocular lens
Part of microscope: transmits image from objective lens to ocular
“Prism”
Body
Part of microscope: Primary lens magnify specimen
Objective lens
Part of microscope: Holds slide in position
Stage
Part of microscope: Focuses light
Condenser
Part of microscope: Controls amount of light entering condenser
Diaphragm
Part of microscope: Light source
Illuminator
Part of microscope: moves stage up and down
Coarse focusing knob
