microscope Flashcards
Uses 2 compound lenses to magnify objects. The lenses _____ light to make the object beneath them appear closer.
Light microscope
refract
What are the three magnification objective lenses
Scanning power
Low power
High power
Focusing Specimens
1. Always start with the _____ objectives.
2. Place your specimen in the ______ of your field of view.
3. Use the _____ to focus.
4. Next, use the _______ until clear.
5. Once you’ve focused on scanning, switch to _________
6. Repeat steps 3-4.
7. Switch to _______.
8. Only use the _____________ to focus specimens.
Scanning
center
Coarse knob
fine knob
low power
High power
Fine Adjustment Knob
- Scanning- use _____ knob
- Low- Use ______ knob
- High- Use _____ knob only.
Coarse &Fine
Coarse &Fine
Fine
Field of view:
The area a person can _____ through a microscope. It is represented by a ______.
As the magnification ________, field of view ________. As you switch from scanning to high, the microscope gets _____.
The lens ______ the image of the object inside the microscope
view
circle
increases
decreases
smaller
inverts
What to do?
Image is too dark!
Adjust the diaphragm, make sure ur light is on.
What to do?
There’s a spot in my viewing field, even when I move the slide, the spot stays in the same place!
Your lens is dirty. Use lens paper, and only lens paper to carefully clean the objective and ocular lens.
What to do?
I can’t see anything under high power!
Remember the steps, if u cant focus under scanning and then low power, u wont be able to focus anything under high power.
What are the types of microscopes?
simple microscope
compound light microscope
electron microscope
scanning electron microscope
transmission electron microscope
phase contrast microscope
ultra centrifuge
it is called simple because it has only 1 lens
simple microscope
it is called compound because it is composed of two lens system
compound light microscope
uses electrons to gain data & view objects at externally high magnification
electron microscope
is able to show contrast in transparent specimens, no staining is required.
phase contrast microscope
spins suspension at high speeds to separate
ultra centrifuge
uses a microscope to dissect very small organisms
microdissection