microscope Flashcards

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1
Q

Uses 2 compound lenses to magnify objects. The lenses _____ light to make the object beneath them appear closer.

A

Light microscope
refract

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2
Q

What are the three magnification objective lenses

A

Scanning power
Low power
High power

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3
Q

Focusing Specimens
1. Always start with the _____ objectives.
2. Place your specimen in the ______ of your field of view.
3. Use the _____ to focus.
4. Next, use the _______ until clear.
5. Once you’ve focused on scanning, switch to _________
6. Repeat steps 3-4.
7. Switch to _______.
8. Only use the _____________ to focus specimens.

A

Scanning
center
Coarse knob
fine knob
low power
High power
Fine Adjustment Knob

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4
Q
  1. Scanning- use _____ knob
  2. Low- Use ______ knob
  3. High- Use _____ knob only.
A

Coarse &Fine
Coarse &Fine
Fine

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5
Q

Field of view:
The area a person can _____ through a microscope. It is represented by a ______.
As the magnification ________, field of view ________. As you switch from scanning to high, the microscope gets _____.
The lens ______ the image of the object inside the microscope

A

view
circle
increases
decreases
smaller
inverts

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6
Q

What to do?

Image is too dark!

A

Adjust the diaphragm, make sure ur light is on.

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7
Q

What to do?

There’s a spot in my viewing field, even when I move the slide, the spot stays in the same place!

A

Your lens is dirty. Use lens paper, and only lens paper to carefully clean the objective and ocular lens.

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8
Q

What to do?

I can’t see anything under high power!

A

Remember the steps, if u cant focus under scanning and then low power, u wont be able to focus anything under high power.

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9
Q

What are the types of microscopes?

A

simple microscope
compound light microscope
electron microscope
scanning electron microscope
transmission electron microscope
phase contrast microscope
ultra centrifuge

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10
Q

it is called simple because it has only 1 lens

A

simple microscope

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11
Q

it is called compound because it is composed of two lens system

A

compound light microscope

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12
Q

uses electrons to gain data & view objects at externally high magnification

A

electron microscope

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13
Q

is able to show contrast in transparent specimens, no staining is required.

A

phase contrast microscope

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14
Q

spins suspension at high speeds to separate

A

ultra centrifuge

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15
Q

uses a microscope to dissect very small organisms

A

microdissection

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16
Q

Two distinctive types of cells

A

prokaryotic
eukaryotic

17
Q

smallest eukaryotic cell

A

RBC

18
Q

a relatively large single celled organism, typically 10-100 micrometer across

A

amoeba