Microscope Flashcards
Magnify image and allow visualization of greater details than is possible with the unaided eye
LIGHT MICROSCOPY
2 CLASSIFICATIONS OF LIGHT MICROSCOPY
Simple
Compound
One lens
Simplest – magnifying glass or pair of reading glasses
Simple Microscope
Multiple lens
Compound Microscope
– the power of the microscope to enlarge the image of an object
Magnification
– ability of the microscope to distinguish two objects from each other
Resolution
PARTS OF MICROSCOPE
Has 2 major parts:
Optical parts
Mechanical parts
– magnifies tissue specimens
Optical parts
– supports the optical parts
Mechanical parts
OPTICAL PARTS
Ocular lens / Eyepiece
Objective lens
lens that one looks into
Magnifies 10x
Ocular lens / Eyepiece
– shortest, red
Scanner (4x)
- yellow
Low power (10x)
- blue
High power (40x)
- longest, b&w
Oil Immersion (100x)
MAGNIFICATION:
Multiply the __________ by the______
eyepiece magnification (10x) by the objective magnification used (4x, 10x, 40x)
Connects ocular tube and base. It also helps carry the microscope
Arm (limb)
Supports the lenses and nosepiece of the microscope and connects to the base of the microscope
Arm
Provides support to help microscope stand upright
Base
Focus of image under high power and moving the stage up and down.
Coarse adjustment knobs
Lower or raise the stage
Used for focus on scanning. Usually, the low power lens is used, enabling the movement of the tube.
Coarse adjustment knobs
Forming a cone of all the dispersed light rays from the illuminator
Condenser
Gathers light from the microscope’s light source and concentrate it into a cone of light that illuminates the specimen
Condenser
Controls the intensity of illuminating light
Diaphragm (Iris)
