MICROPARA_LAB Flashcards

1
Q

A system of accurately ground
lenses arranged to give sharp,
clear, magnified images of
minute objects

A

MICROSCOPE

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2
Q

The technology of making very
small things visible to the
naked eye

A

MICROSCOPY

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3
Q

the process of enlarging something
only in appearance

A

MAGNIFICATION

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4
Q

related to scaling up visuals or
images to be able to see more detail

A

MAGNIFICATION

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5
Q

ability of the lenses to distinguish
fine detail and structure

A

RESOLVING POWER

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6
Q

ability to distinguish two points a
specified distance apart

A

RESOLVING POWER

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7
Q

when two substances have a
different index of refraction, the
light will bend as it passes from
one material to another

A

REFRACTIVE INDEX

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8
Q

Has one lens, similar to a
magnifying glass

A

SIMPLE MICROSCOPE

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9
Q

Leeuwenhoek’s simple
microscopes magnify images
from 100X to 300X

A

SIMPLE MICROSCOPE

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10
Q

Has series of lenses

A

COMPOUND MICROSCOPE

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11
Q

Enables us to examine
very small specimens
and some fine details

A

COMPOUND MICROSCOPE

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12
Q

Microscope uses visible light to
observe specimen

A

LIGHT MICROSCOPY

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13
Q

Specimen appears
against a bright
background

Use: various stained
specimens

A

B R I G H T F I E L D

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14
Q
  • Special condenser with
    opaque disk. Light enters
    the objective lens
  • Use: for microorganisms
    which cannot be stained
A

D A R K F I E L D

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15
Q
  • Uses condenser with annular
    diaphragm which allows direct
    light to pass through the
    condenser
  • Use: detailed examination of
    internal structures; does not
    require staining
A

P H A S E - C O N T R A S T

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16
Q
  • Uses a UV or near-UV
    source of illumination
  • Use: observation of
    natural fluorescence and
    immunofluorescence
A

F L U O R E S E N C E

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17
Q
  • Uses different refractive
    indexes to produce image
  • Use: provides 3D images
    of samples
A

DIFFERENTIAL INTERFERENCE CONTRAST

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18
Q
  • Light source and
    condenser is on top,
    above the stage
  • Use: observation of living
    cells under more natural
    conditions
A

I N V E R T E D

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19
Q

Microscope uses beam of electrons
instead of light

A

ELECTRON MICROSCOPY

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20
Q

Offers much greater resolving power

A

ELECTRON MICROSCOPY

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21
Q

Electrons are reflected from
the specimen; magnifies
1,000 to 10,000X

A

SCANNING ELECTRON (SEM)

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22
Q

Use: study of surface
features of cells and viruses

A

SCANNING ELECTRON (SEM)

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23
Q

Electrons pass through the
specimen; magnifies 10,000
to 100,000X

A

TRANSMISSION ELECTRON (TEM)

24
Q

Use: examination of viruses
or internal ultrastructure in
thin sections of cells

A

TRANSMISSION ELECTRON (TEM)

25
Q

Uses a thin metal probe
that scans a specimen

A

SCANNING TUNNELING (STEM)

26
Q

Use: reveals bumps and
depressions of atoms on
surface of specimen

A

SCANNING TUNNELING (STEM)

27
Q

Measures the deflection
of a laser beam aimed at
the tip of a probe that
travels across the surface
of the specimen

A

ATOMIC FORCE MICROSCOPY

28
Q

Measures the deflection
of a laser beam aimed at
the tip of a probe that
travels across the surface
of the specimen

A

ATOMIC FORCE MICROSCOPY

29
Q

Use: visualization of
individual molecules and
atoms

A

ATOMIC FORCE MICROSCOPY

30
Q

Used when there is a need to
culture microorganisms and
for substances that must be
protected from contamination

A

SCREW CAPPED TUBES

31
Q

Used to make broth, agar
deep, and agar slant

A

SCREW CAPPED TUBES

32
Q

Used to detect production of
gas by microorganisms. An
inverted small tube is initially
filled with the solution in
which the microorganism is
to be grown.

A

DURHAM FERMENTATION TUBE

33
Q

Simpler than Durham tube.
Used also for observing
microorganisms perform
fermentation.

A

SMITH FERMENTATION TUBE

34
Q

used to make agar
plates for microbiology
studies

A

PETRI DISH

35
Q

Glassware calibrated for
precise volume.

A

VOLUMETRIC FLASK

36
Q

Used for measuring or
storing different
amounts of liquid

A

VOLUMETRIC FLASK

37
Q

Has a single graduation
that allows it to deliver
one specific volume
accurately

A

VOLUMETRIC PIPETTE

38
Q

serological or blow-out
pipettes have no 0ml
mark as that corresponds
to an empty pipette

A

SEROLOGIC PIPETTE

39
Q

Makes it easy to deliver
liquids, especially small
volumes, accurately and
precisely

A

PIPETTOR

40
Q

Has a disposable tip

A

PIPETTOR

41
Q

Used to observe
specimens for a long time
because they do not dry
up very quickly. Thus,
motility of an organism
can be observed.

A

HANGING DROP SLIDE

42
Q

AKA smear loop, inoculation
wand or microstreaker

A

INOCULATING LOOP

43
Q

used to retrieve and transfer
an inoculum from a culture of
microorganisms

A

INOCULATING LOOP

44
Q

mainly used to pick a single
colony (pure) and transfer in an
agar slant (stab and streak
method)

A

INOCULATING NEEDLE

45
Q
  • Test tube rack
  • Slant rack
  • Staining rack
A

RACKS

46
Q

Uses dry heat to sterilize
heat-stable materials

A

HOT AIR STERILIZER

47
Q

For sterilization of media
and other materials
affected by moisture

A

HOT AIR STERILIZER

48
Q

Uses moist heat or steam
under pressure

A

AUTOCLAVE

49
Q

For sterilization of media
and other materials
affected by moisture

A

AUTOCLAVE

50
Q

Not sterile and cannot
be used to sterilize
culture media

A

DRYING OVEN

51
Q

For drying specimens
and lab apparatus or
glassware

A

DRYING OVEN

52
Q

used for growing
bacterial cultures or
providing suitable
conditions for a
chemical or biological
reaction.

A

INCUBATOR

53
Q

It is used in the
microbiological lab for
incubations or enabling
chemical reactions to
occur at higher
temperatures

A

WATER BATH

54
Q

Storage for culture media

A

R.REFRIGERATOR

55
Q

Preserves the
morphological
characteristics of
microorganisms while
arresting their growth

A

REFRIGERATOR

56
Q

An enclosed, ventilated
lab workspace for safely
working with pathogens
requiring a defined
biosafety level

A

BIOSAFETY CABINET (BSC)