MICROPARA_LAB Flashcards
A system of accurately ground
lenses arranged to give sharp,
clear, magnified images of
minute objects
MICROSCOPE
The technology of making very
small things visible to the
naked eye
MICROSCOPY
the process of enlarging something
only in appearance
MAGNIFICATION
related to scaling up visuals or
images to be able to see more detail
MAGNIFICATION
ability of the lenses to distinguish
fine detail and structure
RESOLVING POWER
ability to distinguish two points a
specified distance apart
RESOLVING POWER
when two substances have a
different index of refraction, the
light will bend as it passes from
one material to another
REFRACTIVE INDEX
Has one lens, similar to a
magnifying glass
SIMPLE MICROSCOPE
Leeuwenhoek’s simple
microscopes magnify images
from 100X to 300X
SIMPLE MICROSCOPE
Has series of lenses
COMPOUND MICROSCOPE
Enables us to examine
very small specimens
and some fine details
COMPOUND MICROSCOPE
Microscope uses visible light to
observe specimen
LIGHT MICROSCOPY
Specimen appears
against a bright
background
Use: various stained
specimens
B R I G H T F I E L D
- Special condenser with
opaque disk. Light enters
the objective lens - Use: for microorganisms
which cannot be stained
D A R K F I E L D
- Uses condenser with annular
diaphragm which allows direct
light to pass through the
condenser - Use: detailed examination of
internal structures; does not
require staining
P H A S E - C O N T R A S T
- Uses a UV or near-UV
source of illumination - Use: observation of
natural fluorescence and
immunofluorescence
F L U O R E S E N C E
- Uses different refractive
indexes to produce image - Use: provides 3D images
of samples
DIFFERENTIAL INTERFERENCE CONTRAST
- Light source and
condenser is on top,
above the stage - Use: observation of living
cells under more natural
conditions
I N V E R T E D
Microscope uses beam of electrons
instead of light
ELECTRON MICROSCOPY
Offers much greater resolving power
ELECTRON MICROSCOPY
Electrons are reflected from
the specimen; magnifies
1,000 to 10,000X
SCANNING ELECTRON (SEM)
Use: study of surface
features of cells and viruses
SCANNING ELECTRON (SEM)