Microbiology Test

Question 1

What is the purpose of the Microbiology Test?

Answer 1

• For the identification of the causative agent in Meningitis.

Question 2

What is the recovery time for microorganisms in cases of bacterial meningitis and tubercular meningitis when using appropriate culture media?

Answer 2

• Microorganisms must be recovered within 24 hours using the appropriate culture medium
• While for tubercular meningitis, it may take up to 6 weeks for recovery.

Question 3

What is the procedure for specimen concentration in a microbiology test using cerebrospinal fluid (CSF)?

Answer 3

• Involves centrifuging the CSF at 1500g for 15 minutes
• The resulting sediment is used for slides and culture.

Question 4

What is the primary purpose of a CSF culture in microbiology testing?

Answer 4

• Confirmatory rather than diagnostic procedure.

Question 5

What laboratory tests are typically used for a preliminary diagnosis in microbiology?

Answer 5

1. Gram Stain for identifying gram-negative or gram-positive bacteria
2. Acid Fast Stain for detecting mycobacterium
3. India Ink Preparation for detecting Cryptococcus neoformans
4. Latex Agglutination Test for identifying other causative agents of meningitis.

Question 6

What are the predominant cell types seen in the major laboratory results for the differential diagnosis of meningitis for bacterial, viral, tubercular, and fungal infections?

Answer 6

• Bacterial: Neutrophils
• Viral: Lymphocytes
  *tubercular- LYMPHO AND MONO
• Fungal: Lymphocytes, Monocytes, and Eosinophils

Question 7

How would you categorize the level of protein production in bacterial, viral, tubercular, and fungal infections?

Answer 7

• Bacterial: Marked
• Viral: Moderate
• Tubercular: Moderate to Marked
• Fungal: Moderate to Marked

Question 8

What are the different threshold values for lactate levels in the context of bacterial, viral, tubercular, and fungal infections?

Answer 8

• Bacterial: > 35 mg/dL
• Viral: Normal
• Tubercular: > 25 mg/dL
• Fungal: > 25 mg/dL

Question 9

How does the level of glucose in the body typically change in response to bacterial, viral, tubercular, and fungal infections?

Answer 9

• Bacterial: Markedly ↓
• Viral: Normal
• Tubercular: ↓
• Fungal: Normal to ↓