CSF Electrophoresis

Question 1

What is the primary purpose of CSF electrophoresis?

Answer 1

• The primary purpose of CSF electrophoresis is the detection of OLIGOCLONAL BANDS.

Question 2

What can CSF electrophoresis determine regarding a fluid?

Answer 2

• CSF electrophoresis can determine if a fluid is indeed cerebrospinal fluid (CSF).

Question 3

What is the key procedure involved in CSF electrophoresis?

Answer 3

• Involves separating proteins through electrophoresis using cellulose acetate strips with anti-serum
• Followed by washing and staining the strips.

Question 4

What are the key steps involved in the CSF Electrophoresis procedure?

Answer 4

1.Prepare agarose gel (powdered form).
2.fter microwaving, transfer the gel to a molding tray.
3.Use a comb to create wells in the solidifying gel.
4. Apply loading buffer to the created wells.
5.Place samples in the wells.
6. Stain the gel and view the results.

Question 5

What is the most common method used for CSF electrophoresis?

Answer 5

• Agarose Gel Electrophoresis, often followed by Coomassie Brilliant Blue Staining for visualization.