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Spring 2020 Perio > Microbiology of Periodontal Disease > Flashcards

Microbiology of Periodontal Disease Flashcards

1
Q

List and define three types of factors that

contribute to periodontal disease.

A

Local Factors
Systemic Factors
Host Factors

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2
Q

environmental forces that
predispose to inflammatory periodontal
diseases

A

Local Factors

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3
Q

medical conditions
that predispose to inflammatory
periodontal diseases

A

Systemic Factors

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4
Q

the influences of one’s

own body

A

Host Factors

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5
Q

Def: dense, non-mineralized, complex
mass of bacterial colonies living in a gel-
like intermicrobial matrix ž In addition to bacteria, other
microorganisms live in this mass (ex.
Yeasts, viruses)

A

Dental Plaque / Bacteria product

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6
Q

Dental Biofilm microcolony surrounded by ____ matrix.

Different sections of the biofilm will be very different- pH, oxygen tension, and nutrients

A

intermicrobial

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7
Q

Dental Biofilms:
Bacteria in biofilm are NOT distributed evenly

  • They cluster together to form a ____ shaped microcolony
  • Each microcolony is an independent community containing thousands of compatible bacteria
  • Protective extracellular slime-layer- protection from:
    1.
    2.
    3.
A

mushroom

  1. Antibiotics
  2. Antimicrobials
  3. Body’s immune system
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8
Q

Over 700 types of bacteria in the mouth. Only about __ or so are pathogenic for perio

A

20

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9
Q
  • dense cell wall, stain
    purple on crystal violet dye
  • permeable cell wall, do
    not stain
A
  • Gram +

- Gram -

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10
Q
  • Oxygen Using
  • Cannot tolerate oxygen
  • can adjust more flexible
A
  • Aerobic
  • Anaerobic
  • facultative aerobic, facultative anaerobic:
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11
Q
  • stuck where they are

- get around

A
  • non-motile

- motile

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12
Q
  • Utilize carbs as food

- utilize proteins as food obtained in part from the tissue destruction they cause

A
  • Saccharolytic

- Asaccarolytic

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13
Q

Gram +
Aerobic
Non-motile
Saccharolytic

A

HEALTH :)

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14
Q

Gram Negative
Anaerobic
Motile
Assacharolytic

A

Disease :(

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15
Q

4 phases of plaque formation

A
  1. Attachment of bacteria to solid surface
  2. Initial colonization
  3. Secondary colonization
  4. Formation of mature biofilm
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16
Q

4 phases of plaque formation for supraG:

A
  1. Phase 1&2 Attachment and intiital colonization (1-2days of accumulation)
  2. Secondary colonization (2-4days)
  3. Maturation (4-7days) REDNESS SWELLING BOP
  4. MATURATION CONTINUES 7-14 DAYS . MODERATE TO SEVERE INFLAMMATION, BLUISH HUE, CHANGES IN CONSISTENCY
17
Q

subG Biofilms

A

Days 14- 21 days:

  • vibrios and spirochetes remain prevalent in the depths of the mass ž
  • Inflammation of the gingiva caused by
    supraG plaque changes the relationship
    between the gingival margin and the tooth ž Gingival enlargement facilitates
    movement into sulcus ž

-Microbiota of subG: more gram -, more
anaerobic, more motile, more
assacharolytic

18
Q

3 types of subG plaque

A
  • Tooth attached plaque
  • Epithelial attached plaque
  • unattached plaque
19
Q

› Inner layers dominated by gram-positive bacteria › Gram-negative cocci and rods are also present

Is it …

a. Tooth attached plaque
b. Epithelial attached plaque
c. unattached plaque

A

a.

20
Q

› Bacteria are attached to epithelium › Also known as loosely adherent plaque › Layers closest to soft tissue contain large numbers of motile gram-
negative bacteria and spirochetes › Bacteria invade gingival connective tissue and are found within
periodontal connective tissues and on surface of alveolar bone
› This type of plaque is most detrimental to
periodontal tissues**

Is it …

a. Tooth attached plaque
b. Epithelial attached plaque
c. unattached plaque

A

b. epithelial attached plaque

21
Q

› Unorganized gram-negative rods and spirochetes › Separated from epithelium by a layer of leukocyte

Is it …

a. Tooth attached plaque
b. Epithelial attached plaque
c. unattached plaque

A

c. unattached plaque

22
Q

How do we remove each type of subG plaque at the dental office :)

  1. Tooth associated
    (attached)—
    removed by ____
  2. Tissue associated
    (attached to tissue)—
    removed by
    _____
  3. Unattached subg
    plaques (not part of
    biofilm, sometimes
    called planktonic)—
    removed by _____
A
  1. SRP
  2. curretage
  3. flushing
23
Q

what is bacterial aggregation with food debris called

A

materia alba

24
Q

Nature of bacterial plaque is significant;
sulcular ulceration allows bacteria
and/or by products to invade ____ ___

A

gingival

CT

25
Q

Due to things such as tobacoo, alcohol, stress, systemic conditions that alter immune response…

Pathogenic bacteria can produce: 
1. 
2. 
3. 
4.
A
  1. Collagenase: degrades collagen
  2. Hyaluronidase: can increase tissue permeability and
    contribute to bone resporption
  3. Chondroitinase can increase tissue permeability
  4. Proteases: break down noncollagenous proteins and
    increase capillary permeability
26
Q

What pathogenic waste does bacteria secrete

A
  1. Bacterial waste:hydrogen sulfide

2. Toxins : leukotoxin, endotoxins

27
Q

is found in cell wall of Gram –
bacteria
– Include lipopolysaccharides (LPS, LOS), which can
cause tissue necrosis and initiate inflammation and
immune response – Some stimulate bone resorption

A

endotoxin

28
Q

disturbs PMN leukocytes

A

leukotoxin

Spring 2020 Perio (12 decks)

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