Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

BIOL-M 315 > Microbiology Midterm > Flashcards

Microbiology Midterm Flashcards

1
Q

phase microscopy is best used for

A

live cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

bacteria in milk can survive pasteurization temperatures and cause spoilage or souring of milk, these bacteria are called

A

thermodurics

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what allows bacteria to normally survive hypotonic solutions

A

cell wall

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what is the average ratio of bacteria to human cells in the human body

A

10 to 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

osmophiles are organisms that can grow

A

at high concentration of sugar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is the bacterial arrangement term for cocci arranged in clusters

A

staphylococcal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

while trying to view a specimen under the light microscope, turning the coarse or fine focus knows adjusts the distance between which of the following parts

A

the stage and the objective lenses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

these kinds of bacteria use aerobic respiration to make their energy but can only tolerate very low concentrations of oxygen

A

microaerophiles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

some bacteria can grow at 4C and can cause food spoilage they are called

A

psychrotrophs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Gram’s iodine is added to the Gram stain procedure for what reason

A

it combines with the crystal violet and forms an insoluble complex in Gram positive cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

in a typical light microscope, what are the series of lenses that focus light onto the specimen called

A

condenser

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

when we say bacteria are ubiquitous, we mean

A

they are found everywhere

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

IMViC tests are used to

A

differentiate between E. coli and E. aerogenes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what type of slides would you most likely look at with phase-contrast microscopy

A

wet mount slides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what type of media would you use for the growth of fastidious organisms

A

enriched media

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

which type of organism does NOT need oxygen and is NOT poisoned by it

A

aerotolerant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

what are the two types of light rays important for phase contrast microscopy

A

direct rays and diffracted rays

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

which of the following microorganisms would you NOT be able to observe using light microscopy

A

ultramicrobacteria (184 nm long, shorter than other options)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

coliforms have which of the following characteristics

A
  • gram negative
  • facultative anaerobes
  • non-endospore forming rods
  • ferment lactose to produce gas
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

most human pathogens belong to what group of bacteria

A

mesophiles

21
Q

who developed the phase contrast microscope

A

Frits Zernike

22
Q

if you transfer 1 mL of culture into 99mL of sterile water and then add 0.1 mL of that to an agar plate, what is the final dilution of the culture on that plate

A

1: 1000

23
Q

respiration uses what as the terminal electron acceptor

A

oxygen

24
Q

endospores are highly resistant to heat, radiation, acids and many chemicals because of their protective protein coating known as

A

exosporium

25
Q

after the alcohol step in the gram stain procedure, what color will gram negative bacteria be

A

clear

26
Q

why might a bacterial culture give Gram-variable results

A
  • the culture is too old
  • the culture is a Bacillus species
  • the smear is too thick
  • it was over decolorized
27
Q

acid-fast staining is used

A

when the bacteria have a high mycolic acid content

28
Q

Brownian motion is caused by

A

Bombarding molecules

29
Q

as you increase the magnification, __________ decreases

A
  • the field of view

- the working distance

30
Q

which of these tests is not matched correctly with a material used in the test

A

confirmed test …. tryptone glucose extract agar

correct:

  • presumptive test … lactose broth
  • presumptive test … durham dube
  • confirmed test … endo broth
  • confirmed test … EMB agar
31
Q

which of these can NOT be measured by a spectrophotometer

A

Most probable number

32
Q

which of the following environments is most likely to cause plasmolysis

A

hypertonic

33
Q

what’s the most critical step of the gram stain

A

using the alcohol

34
Q

which setting should the condenser be on when looking at bacteria under oil immersion using phase contrast microscopy

A

3 (x100?)

35
Q

which of the following is NOT a part of a bacterial flagellum

A

N-ring

yes:

  • filament
  • L-ring
  • S-ring
  • hook
36
Q

motility medium can be used to determine if bacteria are motile because

A

it has an agar concentration of ~ 0.4%

37
Q

where would you NOT normally find bacteria among the following

A

your blood

38
Q

your light microscope has a limit resolution of (??)

A

0.2 micrometers

39
Q

which of these statements is true

A

-the presence of microorganisms in food doesn’t mean it has potential to cause disease

40
Q

it is easier to test water for presence of indicator bacteria such as

A

E. coli

41
Q

microorganisms used enzymes like catalase and peroxidase to

A

convert toxic oxygen forms to less harmful compounds

42
Q

Thermodurics are bacteria capable of what?

A

survival at high temperature

43
Q

which of these is a reason microbiologists use acid-fast staining

A

able to stain cells with a high lipid content cell wall

44
Q

which of the following is an example of a bacterium that is present in the form of a tetrad cocci

A

micrococcus leteus

45
Q

the telescopic eyepiece is used for

A

aligning the phase rings

46
Q

what is the bacterium that has two bundles of flagella at opposite poles called

A

amphitrichous

47
Q

which of these techniques is used in the isolation of pure bacterial cultures

A

streak plate

48
Q

what is the cell wall of bacteria composed of

A

peptidoglycan

49
Q

what microscope part should never be used with the two highest power objectives

A

coarse focus know

BIOL-M 315 (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions