Bio Final Flashcards

1
Q

positive coagulase test

A

solid clot

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

gram negative rod

A

e. coli, B meg

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

K/K reaction on lactose slant

A

no sugar fermented

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

bacteriostatic

A

inhibits growths of microbes, doesn’t kill them

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

indole positive, gram -, green metallic colonies on EMB

A

E coli

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

plaque forming units

A

4.5 x 10^7

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

blood agar plate, partial breakdown of hemoglobin in RBC –> greenish discoloration, what type of hemolysis

A

alpha

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

antiseptic

A
  • alcohol
  • betadine
  • inhibits microbial growth
  • kills microorganisms

doesn’t damage living tissue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

coag positive, catalase positive, gram positive

A

s aureus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

positive methyl red

A

red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

MacConkey agar selective for ___ and differential for ___

A

lactose fermentation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

barritts A and B associated with

A

voges proskauer test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

downside of assessing bacterial cultures by turbidometric method

A

non viable cells are indistinguishable form viable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

bacteriophage are viruses that infect bacterial cells

A

components:

  • head
  • capsid
  • sheath
  • tail
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

on a tsi slant hydrogen sulfids production would be indicated by ___ precipitate

A

black

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

T/F: s aureus produces a hemolysin called alpha toxin that causes a wide clear zone of beta hemolysis on blood agar

A

true

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

positive catalase test

A

bubbling after addition of reagent to cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

steps of lytic phage infection

A
  • recognition
  • penetration
  • replication
  • release
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

ingredient in MacConkey that makes it selective

A

bile salts, crystal violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

method used to test antimicrobial sensitivity of microorganisms

A

kirby bauer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

(gamma) y-hemolysis on a blood agar plate =

A

no clearing

22
Q

which is true ABOUT thermal death point

A

thermal death point is the lowest temperature at which a population of an organism is killed

23
Q

characteristics of bacteriophage

A
  • Teven phages infect e coli
  • nucleic acid is encased in protein capsid
  • they exhibit specificity to hosts
  • obligate intracellular parasites

-dont: contain metabolic machinery

24
Q

what does enzyme catalase do

A

breaks down toxic H2O2

25
characteristics of endospores
- more resistant to heat stress - contain calcium dipicolinate - produced by bacillus - macromolecules less susceptible to denaturation
26
chem agents and use
- chlorine --- water pathogens - ethylene oxide -- plastic petri dishes - sterilant -- surgical equip - sanitizers -- cooking equipment not: disinfectants-- applied to skin before surgery
27
positive oxidase test, organism has
cytochrome c
28
elevated temp kills microorganism bc of
enzyme denaturing protein unfolding loss of DNA structur
29
function of durham tube
collect gas produced by fermentation
30
bacterial cells that have phage DNA integrated into their DNA
lysogens
31
MacConkey 24 hrs 37c, red colonies
gram -, ferments lactose
32
characteristics of streptococcus
- facultative anaerobe - gram + - chains - spherical doesn't produce catalase
33
disinfectants
chem agents applied to inanimate objects to kill microorganisms
34
of bacteria/mL of an original culture if you transferred 1 mL of culture into 99 mL of sterile water and add .1 mL to agar and get 265 colonies
2.65x10^5
35
enteric bacteria
- faculatative anaerobes - gram - - rods - in intestines not spore former
36
distinguishing factor that strep are separated into by lancefield groups
amount of hemolysis
37
former name of HAIs
nosocomial infections
38
spectrophotometer is used to measure optical density , measurement of
turbidity
39
organism correctly matched with metabolism relationship to oxygen
aerotolerant -- only ferments
40
in order to replicate a virus will
hijack host machinery
41
indole
cleavage product of tryptophan
42
nose swab, streak on mannitol salt agar, reddish
cannot ferment mannitol
43
T/F: thermal death time is the lowest temperature at which a population of a target organism is killed in 10 minutes
f
44
you dilute 1 ml from a 10 ml starter culture into 99 ml of liquid, total dilution is
10^-2A
45
gram -, coccus, oxidase +
neisseria
46
viral structure that contains genome
nucleocapsid
47
tsi slant, fermentation
red to yellow
48
characteristics of staph
halotolerant - gram + - non-spore forming - part of human microbiota not motile
49
zone of inhibition
diameter, in mm
50
positive oxidase test
reagent turns purple on end of swab