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Lab Procedures 1 > Microbiology Exam 1 > Flashcards

Microbiology Exam 1 Flashcards

1
Q

Microorganisms normally found on human body. Assists in prevention of disease causing microorganisms.

A

Normal flora

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2
Q

Lactobacillus

A

Protective normal flora

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3
Q

Disease that occurs when pathogenic microorganisms invade body

A

Infection

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4
Q

Smallest microorganisms

Consist of RNA/DNA

A

viruses

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5
Q

Visible masses of bacteria formed on culture medum

A

Colonies

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6
Q

Parasites & types of parasites

A

Helminths, arthropods, protozoa

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7
Q

3 classifications of bacteria and their shapes

A

Cocci (round)
Bacilli ( rod shaped)
Spirilla (spriral)

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8
Q

Regards to all specimen collections, how can results be good?

A

Results are only as good as the specimen that was collected

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9
Q

Ideal time to collect a microbiology specimen?

A

Before antibiotics have begun

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10
Q

Sites for collection of microbiology specimens?

A 
Eyes
Rectum
Nose
Wounds
Throat
Urogenital area
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11
Q

Provide conditions the microorganisms need to survive during transport.

A

Transport systems

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12
Q

MA role in microbiology lab testing

A

Collecting microbilogic specimens (throat, nose, blood, wound)

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13
Q

Throat culture procedures

A

Most common procedure
Visualize back of throat
Patient sitting

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14
Q

Why are blood cultures ordered by providers?

A

Patient has fever of unknown origin

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15
Q

Collection kits for transporting fecal ova and parasite specimens

A

Formalin container

Must be filled to designated line with fecal specimen

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16
Q

Gram stain procedure

A

Differentiating bacteria by how it picks up stain

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17
Q

Primary stain, purple, 1st stained used, 1 minute

A

Crystal violet

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18
Q

Grams iodine-mordant-to hold

A

Holding crystal violet to gram stain organisms

19
Q

Most critical step, alcohol or acetone

A

Decolorizer

20
Q

Safranin-counterstain

A

Last step

21
Q

What is done between each step?

A

Rinse slide with water

22
Q

Gram-positive is what stain color?

A

Purple

23
Q

Gram negative

A

Pink/red

24
Q

Smear

A

Smear portion of specimen onto slide

25
Q

Rapid techniques done to view organisms in their living state
For trichomonis, vaginalis
Glass slide, coverslip, gloves, drop of saline and specimen

A

Wet mounts

26
Q

Koh

A

Looking for yeast

Potassium hydroxide

27
Q

Cellulose tape test

A

Sticky side down over persons anal area

Sticky side down onto slide

28
Q

Hemolysis

A

Alpha
Beta
Gamma

29
Q

Alpha

A

Incomplete

Green around colonies

30
Q

Beta

A

Complete hemolysis

Clear around colonies

31
Q

Gamma

A

No toxin is present

No hemolysis seen around colonies

32
Q

Bacitracin

A

Identify strep A, antibiotic, sensitivity testing

33
Q

Influenza and antigenic drift and shift

A

Drift-gradual change in virus strain

Shift- abrupt change

34
Q

Enterobius vermiculars

A

Parasite-roundworm
Cellulose tape test
Pinworm-disease

35
Q

Neisseria gonorrhoeae

A

Gram negative diplococcus
JEMBEC, swab gram stain culture
gonorrhea

36
Q

Gardnerella vaginlais

A

Gram negative coccobacillus
Wet mount
Bacterial vaginosis

37
Q

Chlamydia trachomatis

A

Gram negative intracellular bacterium
DNA probe test
Chlamydia

38
Q

Trichomonas vaginalis

A

Pear shaped protozoa
Wet mount
T. Vaginalis

39
Q

Candida albicans

A

Koh prep
Oral thrush
Yeast infection

40
Q

Mycobacterium tuberculosis

A

Acid fast branching bacilli
Sputum, acid fast stain, skin test
Tuberculosis

41
Q

Streptococcus phones

Group A Strep

A

Gram positive cocci
Rapid strep test, throat culture
Strep throat

42
Q

Most common species if staphylococcus

A

Epidermidis and aureus

43
Q

Epidermidis

A

Normally found on surface of skin and mucous membrane of mouth

44
Q

Aureus

A

Boils, carbuncle, pimples, impetigo…

Lab Procedures 1 (5 decks)

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