Microbiology Exam 1

Question 1

Microorganisms normally found on human body. Assists in prevention of disease causing microorganisms.

Answer 1

Normal flora

Question 2

Lactobacillus

Answer 2

Protective normal flora

Question 3

Disease that occurs when pathogenic microorganisms invade body

Answer 3

Infection

Question 4

Smallest microorganisms

Consist of RNA/DNA

Answer 4

viruses

Question 5

Visible masses of bacteria formed on culture medum

Answer 5

Colonies

Question 6

Parasites & types of parasites

Answer 6

Helminths, arthropods, protozoa

Question 7

3 classifications of bacteria and their shapes

Answer 7

Cocci (round)
Bacilli ( rod shaped)
Spirilla (spriral)

Question 8

Regards to all specimen collections, how can results be good?

Answer 8

Results are only as good as the specimen that was collected

Question 9

Ideal time to collect a microbiology specimen?

Answer 9

Before antibiotics have begun

Question 10

Sites for collection of microbiology specimens?

Answer 10

Eyes
Rectum
Nose
Wounds
Throat
Urogenital area

Question 11

Provide conditions the microorganisms need to survive during transport.

Answer 11

Transport systems

Question 12

MA role in microbiology lab testing

Answer 12

Collecting microbilogic specimens (throat, nose, blood, wound)

Question 13

Throat culture procedures

Answer 13

Most common procedure
Visualize back of throat
Patient sitting

Question 14

Why are blood cultures ordered by providers?

Answer 14

Patient has fever of unknown origin

Question 15

Collection kits for transporting fecal ova and parasite specimens

Answer 15

Formalin container

Must be filled to designated line with fecal specimen

Question 16

Gram stain procedure

Answer 16

Differentiating bacteria by how it picks up stain

Question 17

Primary stain, purple, 1st stained used, 1 minute

Answer 17

Crystal violet

Question 18

Grams iodine-mordant-to hold

Answer 18

Holding crystal violet to gram stain organisms

Question 19

Most critical step, alcohol or acetone

Answer 19

Decolorizer

Question 20

Safranin-counterstain

Answer 20

Last step

Question 21

What is done between each step?

Answer 21

Rinse slide with water

Question 22

Gram-positive is what stain color?

Answer 22

Purple

Question 23

Gram negative

Answer 23

Pink/red

Question 24

Smear

Answer 24

Smear portion of specimen onto slide

Question 25

Rapid techniques done to view organisms in their living state For trichomonis, vaginalis Glass slide, coverslip, gloves, drop of saline and specimen

Answer 25

Wet mounts

Question 26

Koh

Answer 26

Looking for yeast | Potassium hydroxide

Question 27

Cellulose tape test

Answer 27

Sticky side down over persons anal area | Sticky side down onto slide

Question 28

Hemolysis

Answer 28

Alpha Beta Gamma

Question 29

Alpha

Answer 29

Incomplete | Green around colonies

Question 30

Beta

Answer 30

Complete hemolysis | Clear around colonies

Question 31

Gamma

Answer 31

No toxin is present | No hemolysis seen around colonies

Question 32

Bacitracin

Answer 32

Identify strep A, antibiotic, sensitivity testing

Question 33

Influenza and antigenic drift and shift

Answer 33

Drift-gradual change in virus strain | Shift- abrupt change

Question 34

Enterobius vermiculars

Answer 34

Parasite-roundworm Cellulose tape test Pinworm-disease

Question 35

Neisseria gonorrhoeae

Answer 35

Gram negative diplococcus JEMBEC, swab gram stain culture gonorrhea

Question 36

Gardnerella vaginlais

Answer 36

Gram negative coccobacillus Wet mount Bacterial vaginosis

Question 37

Chlamydia trachomatis

Answer 37

Gram negative intracellular bacterium DNA probe test Chlamydia

Question 38

Trichomonas vaginalis

Answer 38

Pear shaped protozoa Wet mount T. Vaginalis

Question 39

Candida albicans

Answer 39

Koh prep Oral thrush Yeast infection

Question 40

Mycobacterium tuberculosis

Answer 40

Acid fast branching bacilli Sputum, acid fast stain, skin test Tuberculosis

Question 41

Streptococcus phones | Group A Strep

Answer 41

Gram positive cocci Rapid strep test, throat culture Strep throat

Question 42

Most common species if staphylococcus

Answer 42

Epidermidis and aureus

Question 43

Epidermidis

Answer 43

Normally found on surface of skin and mucous membrane of mouth

Question 44

Aureus

Answer 44

Boils, carbuncle, pimples, impetigo...