Microbiology 2 Flashcards

1
Q

Requires oxygen

A

Aerobic

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2
Q

Can grow and function without oxygen

A

Anaerobic

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3
Q

Can provide beef/yeast extracts (peptone,mineral salts)

A

Enriched media

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4
Q

Requires specific additional additives & conditions for growth (gonorrhea)

A

Fastidious

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5
Q

Solid, liquid, semi solid are examples of

A

Forms of media

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6
Q

Gelatin like seaweed product that is liquid when heated and solid when cooled in petri dish

A

Agar

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7
Q

Equipment used in a microbiology lab

A

Incubator, autoclave, safety hood, refrigerator, microscopes

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8
Q

Transfer microorganisms into or on a culture medium for growth

A

Inoculation

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9
Q

Loops have bubble wand, needle is pointed at end

A

Inoculation loops/needles

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10
Q

C & S

A

Culture and sensitivity

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11
Q

Isolation technique/quadrant technique

A

1st quadrant is a swab and last 3 are loops

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12
Q

Used for antibiotic sensitivity testing and counting colonies

A

Colony count/lawn technique

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13
Q

Culture plate incubation period

A

24 hrs

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14
Q

Incubator temp

A

37 degrees C

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15
Q

Determines which antibiotic to use to treat bacterial infections

A

Sensitivity testing

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16
Q

What is kirby-bauer method

A

Sensitivity test

17
Q

Zones of inhibition

A

No growth

18
Q

Escherichia coli

A

E. Coli , gram negative bacilli, cause of UTI

19
Q

Sensitivity testing steps

A
Inoculate liquid
Spread bacteria over whole plate
Kirby Bayer antibiotic dropped on plate
Incubate overnight
Check for zones of inhibition
20
Q

Staphylococcus aureus

A

Gram positive cocci in clusters (cause of wind infections)

21
Q

Reasons for emerging infectious diseases to rise in recent years

A

Growth of human population, increase in international travel, reflect of environmental changes

22
Q

Fine particles suspended in air

A

Aerosols

23
Q

Morbidity vs mortality

A

Morbidity is rate of illness

Mortality is rate of death

24
Q

Variola major and minor

A

Smallpox
Major is most virulent strain (blisters and high fever)
Minor is more mild form, deathbrate less than 1%

25
Q

Killed more people than any other disease

300 million deaths I’m 20th century

A

Smallpox

26
Q

Most lethal disease known to humans

A

Viral hemorrhagic fever

27
Q

One cause of hemorrhagic fever ( direct contact)

A

Ebola virus

28
Q

Neurotoxin botulinum, most poisonous natural substance known (dry mouth, dialated pupils)

A

Clostridium botulinum

29
Q

Caused by e. Coli 0157:H7

A

Hemolytic uremic syndrome

30
Q

Sever respiratory caused by coronavirus

A

SARS

31
Q

“Black death” black spots on skin, 1/3 population of Europe was killed in 1891, affects rodents

A

Plague

32
Q

Yersinia pestis

A

Causitive agent of the plague, transferred by flea bites

33
Q

F. Tularensis, gram negative bacilli

A

Tularemia

34
Q

F. Tularensis, gram negative

A

Tularemia

35
Q

Anthrax

A

Old disease, 5th plague of bible, bacterial spores found in soil

36
Q

Bacillus anthracis

A

Causitive agent of anthrax, gram positive bacillus