Microbiology 2

Question 1

Requires oxygen

Answer 1

Aerobic

Question 2

Can grow and function without oxygen

Answer 2

Anaerobic

Question 3

Can provide beef/yeast extracts (peptone,mineral salts)

Answer 3

Enriched media

Question 4

Requires specific additional additives & conditions for growth (gonorrhea)

Answer 4

Fastidious

Question 5

Solid, liquid, semi solid are examples of

Answer 5

Forms of media

Question 6

Gelatin like seaweed product that is liquid when heated and solid when cooled in petri dish

Answer 6

Agar

Question 7

Equipment used in a microbiology lab

Answer 7

Incubator, autoclave, safety hood, refrigerator, microscopes

Question 8

Transfer microorganisms into or on a culture medium for growth

Answer 8

Inoculation

Question 9

Loops have bubble wand, needle is pointed at end

Answer 9

Inoculation loops/needles

Question 10

C & S

Answer 10

Culture and sensitivity

Question 11

Isolation technique/quadrant technique

Answer 11

1st quadrant is a swab and last 3 are loops

Question 12

Used for antibiotic sensitivity testing and counting colonies

Answer 12

Colony count/lawn technique

Question 13

Culture plate incubation period

Answer 13

24 hrs

Question 14

Incubator temp

Answer 14

37 degrees C

Question 15

Determines which antibiotic to use to treat bacterial infections

Answer 15

Sensitivity testing

Question 16

What is kirby-bauer method

Answer 16

Sensitivity test

Question 17

Zones of inhibition

Answer 17

No growth

Question 18

Escherichia coli

Answer 18

E. Coli , gram negative bacilli, cause of UTI

Question 19

Sensitivity testing steps

Answer 19

Inoculate liquid
Spread bacteria over whole plate
Kirby Bayer antibiotic dropped on plate
Incubate overnight
Check for zones of inhibition

Question 20

Staphylococcus aureus

Answer 20

Gram positive cocci in clusters (cause of wind infections)

Question 21

Reasons for emerging infectious diseases to rise in recent years

Answer 21

Growth of human population, increase in international travel, reflect of environmental changes

Question 22

Fine particles suspended in air

Answer 22

Aerosols

Question 23

Morbidity vs mortality

Answer 23

Morbidity is rate of illness

Mortality is rate of death

Question 24

Variola major and minor

Answer 24

Smallpox
Major is most virulent strain (blisters and high fever)
Minor is more mild form, deathbrate less than 1%

Question 25

Killed more people than any other disease

300 million deaths I’m 20th century

Answer 25

Smallpox

Question 26

Most lethal disease known to humans

Answer 26

Viral hemorrhagic fever

Question 27

One cause of hemorrhagic fever ( direct contact)

Answer 27

Ebola virus

Question 28

Neurotoxin botulinum, most poisonous natural substance known (dry mouth, dialated pupils)

Answer 28

Clostridium botulinum

Question 29

Caused by e. Coli 0157:H7

Answer 29

Hemolytic uremic syndrome

Question 30

Sever respiratory caused by coronavirus

Answer 30

SARS

Question 31

“Black death” black spots on skin, 1/3 population of Europe was killed in 1891, affects rodents

Answer 31

Plague

Question 32

Yersinia pestis

Answer 32

Causitive agent of the plague, transferred by flea bites

Question 33

F. Tularensis, gram negative bacilli

Answer 33

Tularemia

Question 34

F. Tularensis, gram negative

Answer 34

Tularemia

Question 35

Anthrax

Answer 35

Old disease, 5th plague of bible, bacterial spores found in soil

Question 36

Bacillus anthracis

Answer 36

Causitive agent of anthrax, gram positive bacillus