Microbiology ๐ฆ Flashcards
What is the shape and stain of staphylococci?
Gram-positive cocci
What is the arrangement of staphylococci?
In Clusters
Are staphylococci motile?
No
Are staphylococci spore-forming?
No
What is the capsule of staphylococci?
- S. aureus forms microcapsule
- Other species may produce slime layer
What are staphylococci classified into?
Coagulase positive: S. aureus.
Coagulase-negative: S. epidermidis and S. saprophyticus.
What are the samples used for the diagnosis of staphylococcus aureus?
Differ according to the site of infection (swabs, pus, sputum, C.S.F, blood, โฆ).
What are the procedures of diagnosis of Staph aureus?
1- Sample
2- Direct film
3- Culture
4- Identification of colonies
Direct film of Staph aureus
Stained with Gram stain for characteristic morphology.
Culture of staph aureus
- Facultative anaerobes, optimum temperature: 37 ยฐC, normal atmospheric CO2
- Can grow on ordinary media.
- S. aureus produce golden yellow endopigments.
- S. aureus causes ฮฒ-hemolysis on blood agar.
- Selective media is mannitol salt agar, where S.aureus ferments mannitol and salt inhibits other normal flora
How are colonies of staph aureus identified?
- Film stained by Gram for characteristic morphology.
- Biochemical reactions:
โ All staphylococci are catalase positive.
โ S. aureus is coagulase and DNAase positive and ferment mannitol.
What is catalase test used for?
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What is the principle of catalase test?
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What is the method of catalase test?
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What is the interpretation of catalase test?
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what is coagulase test used for?
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What is the principle of coagulase test?
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What are the types of coagulase?
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What is DNAse agar used for?
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What is the principle of DNAse agar?
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What is the method of DNAse agar?
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What is the interpretation of DNAse agar?
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What is the significance of antimicrobial susceptibility?
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What is the significance of phage typing?
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What is a morphology of streptococci?
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What are the culture characters of streptococci?
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What is the difference between S. Pneumoniae and S. Viridans
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What is the classification of streptococci?
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What are the culture characters of streptococci?
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What is the media of streptococci?
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How is growth of streptococci identified?
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Serology of streptococci
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What is the method of bacitracin sensitivity test?
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What is the interpretation of bacitracin test
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How is the scarlet fever diagnosed?
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What is the principal and the method of Schultz-Charlton test?
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What are the uses of dick test?
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What is the principle of dick test?
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How is rheumatic fever diagnosed?
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What does a titer of 200 or more of ASO Todd units indicate?
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How is ASO titer measured?
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What are specimens used for diagnosis of Streptococcus pneumoniae?
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What are the characteristic morphology of streptococcus pneumoniae?
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What are the culture characters of Streptococcus pneumonia?
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How is growth of streptococcus pneumoniae identified?
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Pathogenicity to mice test
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Quellung reaction
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