Microbiology 1 - Exam 2 Flashcards
Escherichia Testing Outcomes
Indole: +
MR: +
VP: -
Citrate: -
TSI: A/AG
Urease: -
Lactose: F
Decarboxylase: L+
MUG: +
ONPG: N/A
Klebsiella Testing Outcomes
Indole: - (K. oxytoca = +)
MR: -
VP: +
Citrate: +
TSI: A/AG
Urease: +
Lactose: F
Decarboxylase: L+, O-
MUG: +
ONPG: +
Citrobacter Testing Outcomes
Indole: Freundii -, koseri +
MR: +
VP: -
Citrate: +
TSI: A/AG, H2S
Urease: V
Lactose: F
Decarboxylase: L-,O-
MUG: N/A
ONPG: N/A
Serratia Testing Outcomes
Indole: -
MR: V
VP: V
Citrate: +
TSI: A/A (+/-G)
Urease: +
Lactose: F
Decarboxylase: N/A
MUG: N/A
ONPG: N/A
Enterobacter Testing Outcomes
Indole: -
MR: -
VP: +
Citrate: +
TSI: A/A (+/-G)
Urease: -
Lactose: F
Decarboxylase: Triple (-)
MUG: N/A
ONPG: +
Pantoea Testing Outcomes
Lactose: F
Salmonella Testing Outcomes
Indole: -
MR: +
VP: -
Citrate: -
TSI: K/AG, H2S
Urease: -
Lactose: NF
Decarboxylase: L+
MUG: -
ONPG: -
Shigella Testing Outcomes
Indole: V
MR: +
VP: -
Citrate: -
TSI: K/A
Urease: -
Lactose: NF
Decarboxylase: L- (S. sonnie = O+)
MUG: N/A
ONPG: - (S. sonnie = +)
Proteus Testing Outcomes
Indole: (P. vulgaris +), (P. mirabilis -)
MR: ++
VP: - -
Citrate: - (v), + (V)
TSI: K/A, H2S
Urease: + strong
Lactose: NF
Decarboxylase: O+ (P. mirabalis)
MUG: N/A
ONPG: N/A
Morganella Testing Outcomes
Indole: +
MR: +
VP: -
Citrate: -
TSI: K/AG
Urease: +
Lactose: NF
Decarboxylase: N/A
MUG: N/A
ONPG: N/A
Providencia Testing Outcomes
Indole: +
MR: +
VP: -
Citrate: +
TSI: K/A (+/-G)
Urease: -
Lactose: NF
Decarboxylase: N/A
MUG: N/A
ONPG: N/A
Yersinia Pestis Testing Outcomes
Indole: -
MR: +
VP: -
Citrate: -
TSI: K/K
Urease: -
Lactose: NF
Decarboxylase: O-
MUG: N/A
ONPG: N/A
Enterobacteriaceae Testing Outcomes
Almost all
Catalase: +
Nitrate: +
Oxidase: -
What microbes use the Phenylalanine Deaminase Test (PAD test)
Proteus
Morganella
Providencia
*Note: most stool pathogens are urease negative.
Virulence factors and associated diseases of E. Coli.
- Virulence factors: Endotoxin (LPS – Lipid A + O polysaccharide (surface antigen)), K
antigen (polysaccharide capsule), H antigen, Pili, cytotoxin and enterotoxins - Enterovirulent E. coli: 6 types, STEC (EHEC), ETEC, EPEC, EIEC, EaggEC and DAEC
(diffusively adhering E. coli) - opportunistic pathogen (UTI, wound, bacteremia or septicemia)
- Nosocomial infections