Micro Clinical Review Flashcards
Gram negative, rod-shaped, and facultative anaerobe.
Catalase: +
Oxidase: -
Indole: +
MR: +
VP: -
Citrate: -
Nitrate: +
PYR: -
TSI: A/AG
Urease: -
Lactose: F
Decarboxylase: L+
MUG: +
ONPG: N/A
Escherichia Coli
Opportunistic, causes UTI, neonatal meningitis, wound infections, and septicemia.
Gram negative, rod-shaped, encapsulated, nonmotile.
Oxidase: -
Indole: -
MR: -
VP: +
Citrate: +
Nitrate: +
TSI: A/AG
Urease: +
Lactose: F
Decarboxylase: L+, O-
MUG: +
ONPG: +
Klebsiella pneumonia
Enterobacteriaceae family. Causes nosocomial infections including UTI, bloodstream infections, lower respiratory tract, cardiovascular, wound, ear, nose, and throat infections.
Gram negative rods. Motile.
Oxidase: -
Indole: V
MR: +
VP: -
Citrate: +
Nitrate: +
TSI: A/AG, H2S
Urease: V
Lactose: F (late)
Decarboxylase: L-,O-
MUG: N/A
ONPG: N/A
Citrobacter freundii
Enterobacteriaceae family. Natural intestinal microflora, but causes UTI.
Gram negative rods. Motile. Facultative anaerobe.
Oxidase: -
Indole: -
MR: V
VP: V
Citrate: +
Nitrate: +
TSI: A/A (+/-G)
Urease: +
Lactose: F
Decarboxylase: N/A
MUG: N/A
ONPG: N/A
Serratia marcescens
Family Enterobacteriaceae. Opportunistic, causing nosocomial respiratory, UTI, or blood infections.
Red pigment called prodigiosin.
Gram negative rod.
Indole: -
MR: -
VP: +
Citrate: +
TSI: A/A (+/-G)
Urease: -
Lactose: F
Decarboxylase: Triple (-)
MUG: N/A
ONPG: +
Enterobacter
Lactose fermenter
Pantoea
Gram negative rods, peritrichous flagella (motile).
Indole: -
MR: +
VP: -
Citrate: -
TSI: K/AG, H2S
Urease: -
Lactose: NF
Decarboxylase: L+
MUG: -
ONPG: -
Salmonella
Causes salmonellosis, typhoid fever (S. typhi), or paratyphoid fever (S. paratyphi).
Gram negative rods, non-motile, non-spore forming.
Oxidase: -
Indole: V
MR: +
VP: -
Citrate: -
Nitrate: +
TSI: K/A
Urease: -
Lactose: NF
Decarboxylase: L- (sonnie = O+)
MUG: N/A
ONPG: - (sonnie = +)
Shigella
Enterobacteriaceae family. Causes shigellosis, traveler’s diarrhea.
Gram negative, rod-shaped, facultative anaerobe. Swarms on colonies (motile).
Oxidase: -
Indole: -
MR: ++
VP: - -
Citrate: - (v)
Nitrate: +
TSI: K/A, H2S
Urease: + strong
Lactose: NF
Decarboxylase: O+
MUG: N/A
ONPG: N/A
Proteus mirabilis
Causes cystitis and pyelonephritis.
Indole: +
MR: +
VP: -
Citrate: -
TSI: K/AG
Urease: +
Lactose: NF
Decarboxylase: N/A
MUG: N/A
ONPG: N/A
Morganella
Indole: +
MR: +
VP: -
Citrate: +
TSI: K/A (+/-G)
Urease: -
Lactose: NF
Decarboxylase: N/A
MUG: N/A
ONPG: N/A
Providencia
Indole: -
MR: +
VP: -
Citrate: -
TSI: K/K
Urease: -
Lactose: NF
Decarboxylase: O-
MUG: N/A
ONPG: N/A
Yersinia Pestis
Almost all
Catalase: +
Nitrate: +
Oxidase: -
Enterobacteriaceae
What microbes use the Phenylalanine Deaminase Test (PAD test)
Differentiates Proteus, Morganella, and Providencia from other Enterobacteriaceae.
Test determines if the organism is able to produce deaminase enzyme, which removes the amine group phenylalanine. When it is removed, it is released as ammonia and phenyl pyruvic acid is produced.
Positive = dark green
Negative = No color change
*Note: most stool pathogens are urease negative.
What are the principles of the: catalase test?
The production of catalase turns hydrogen peroxide into water and oxygen.
What are the principles of the: Oxidase test?
What are the principles of the: Coagulase test?
What are the principles of the: DNAse test?
The ability of an organism to digest DNA.
What are the principles of the: Hippurate hydrolysis test?
What are the principles of the: Bile esculin hydrolysis test?
What are the principles of the: CAMP test?