MICROBIO LAB EXP 7 MIDTERM

Question 1

What is the ova of Ascaris spp. characterized by?

Answer 1

Oval shape with a thick, albuminous outer layer.

Question 2

Describe the ova of Enterobius vermicularis.

Answer 2

Oval with a flattened side, typically containing a single cell.

Question 3

What does the ova of Necator americanus look like?

Answer 3

Elliptical and thin-walled, with a clear space inside.

Question 4

How can you identify the ova of Trichuris trichura?

Answer 4

Football-shaped with polar plugs at each end.

Question 5

Describe the tissue sections of Trichinella spiralis.

Answer 5

Encysted larvae within muscle tissue.

Question 6

What are the identifying features of adult Wuchereria bancrofti?

Answer 6

Long, slender, white worms; can be seen in lymphatic vessels.

Question 7

What does the ova of Diphyllobothrium latum look like?

Answer 7

Operculated, oval, and large with a yellowish color.

Question 8

How do you differentiate between the ova of Taenia saginata and Taenia solium?

Answer 8

Taenia saginata has a larger oval shape; Taenia solium has a more rounded shape.

Question 9

What are the features of the scolex and proglottids of Taenia solium?

Answer 9

Scolex has hooks and suckers; proglottids are segmented.

Question 10

Identify the adult Echinococcus granulosus.

Answer 10

Small, with a scolex and 3-4 proglottids.

Question 11

Describe the ova of Clonorchis sinensis.

Answer 11

Oval with a distinct operculum.

Question 12

What does the ova of Fasciola hepatica look like?

Answer 12

Large, oval-shaped with a distinct operculum.

Question 13

How can you identify the ova of Schistosoma species?

Answer 13

Distinctive spine on one side; varies by species.

Question 14

What are miracidia, rediae, and cercaria of Schistosoma?

Answer 14

Larval stages; miracidia are ciliated, rediae are elongated, cercaria are free-swimming.

Question 15

Describe adult Clonorchis sinensis.

Answer 15

Leaf-shaped, flat, and has two suckers.

Question 15

Identify the adult female and male Schistosoma japonicum.

Answer 15

Females are shorter and live in the male’s groove; males are larger.

Question 16

What are the distinguishing features of Plasmodium species?

Answer 16

Ring forms in red blood cells; varying stages of development.

Question 17

How do you recognize trypanosomes in stained blood smears?

Answer 17

Long, slender bodies with undulating membranes.

Question 18

Identify Entamoeba histolytica in iron-hematoxylin stained fecal smears.

Answer 18

Trophozoites with ingested red blood cells.

Question 19

What does Giardia lamblia look like in iron-hematoxylin stained fecal smears?

Answer 19

Pear-shaped trophozoites with two nuclei.

Question 20

Identify Trichomonas vaginalis in a Papanicolaou-stained smear.

Answer 20

Pear-shaped with a flagellated margin.

Question 21

What are the characteristics of Toxoplasma gondii in tissue sections?

Answer 21

Small, crescent-shaped organisms within cells.

Question 22

Describe adult Pediculus humanus capitis.

Answer 22

Small, grayish-white, wingless insect with a flattened body.

Question 23

How do you identify P. humanus eggs on a hair shaft?

Answer 23

Tiny, oval, and white, firmly attached to hair.

Question 24

What does the itch mite (Sarcoptes scabiei) look like?

Answer 24

Small, round, and white; burrows in the skin.

Question 25

What is the first step in examining the specimens?

Answer 25

Examine various specimens provided.

Question 26

What characteristics should you focus on during your examination?

Answer 26

Relative sizes, shapes, and obvious features like scolex, proglottids, and sex organs.

Question 27

What is under the category of nematodes?

Answer 27

Ova of Ascaris spp.
Ova of Enterobius vermicularis
Ova of Necator americanus
Ova of Trichuris trichura
Tissue sections of Trichinella spiralis
Adult worms of Wechereria bancrofti

Question 28

What is under the category of Plathelyminths (Cestodes or tapeworms?)

Answer 28

Ova of Diphyllobothrium latum
Ova of Taenia saginata and Taenia solium
Scolex and proglotidds of Taenia solium
Adult Echinococcus granulosus

Question 29

What is under the category of Platyhelminths (Trematoes or flukes)?

Answer 29

Ova of Clonorchis sinensis
Ova of Fasciola hepatica
Ova of Schistosoma haematobium, S. japonicum, S. mansonii
Miracidia, rediae and drecaria of Schistosoma
Cercaria of Schistosoma japonicum
Adult female and male Schistosoma jopanicum
Adult Clonoris sinensis

Question 30

What are under the category of Pathogenic Protozoa

Answer 30

Plasmodium vivax, P. falciparum; P. malariae
Trypanosomes in stained blood smears
Entamoeba histolytica in iron-hematoxylin stained fecal smears
Giardia lamblia in iron-hematoxylin stained fecal smears
Trichomonas vaginalis in a papanicolau stained smear form the cervix of uterus
Toxoplasma gondii in hematoxylin and eosin-stained section of tissue