Microbial Growth Flashcards

1
Q

Metabolism

A

Energy
• Exergonic vs. Endergonic
• Anabolism vs. Catabolism

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2
Q

Catabolism

A

large molecules are broken down into smaller ones, releasing energy

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3
Q

Anabolism

A

small molecules are assembled into larger ones, using energy

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4
Q

Chemotrophs
Chemoautotophs

A

Chemical
Inorganic
Hydrogen, sulfur, iron, nitrogen, and carbon monoxide-oxidizing bacteria

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5
Q

Chemotrophs
Chemoheterotrophs

A

Chemical
Organic Compounds
All animals, most fungi, protozoa, and bacteria

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6
Q

Phototrophs
Photoautotrophs

A

Light
Inorganic
All plants, algae, Cyanobacteria, and green and purple sulfur bacteria

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7
Q

Phototrophs
Photohetertrophs

A

Light
Organic compounds
Green and purple nonsulfur bacteria, heliobacteria

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8
Q

Oxidation/Reduction reactions

A

OIL RIG

Electron Carriers
• NAD+/NADH
• NADP+/NADPH
• FAD/FADH2

Adenosine triphosphate
• Energy currency

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9
Q

Enzymes

A

• Act as catalysts
• Bind to substrates
• Lower activation energy

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10
Q

Cofactors and Coenzymes

A

Cofactors
• Inorganic ions
• Fe2+, Mg2+

Coenzymes
• Organic molecules
• Dietary vitamins

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11
Q

Aponenzyme

A

becomes active by binding of coenzyme or cofactor to enzyme.

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12
Q

Holoenzyme

A

is formed when associated cofactor or coenzyme binds to the enzyme’s active site.

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13
Q

Enzyme
Competitive inhibitors

A

• Bind to active site
• Must be equal to substrate concentration

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14
Q

Enzyme
Noncompetitive inhibitor

A

• aka: allosteric inhibitor
• Bind to allosteric site
• Change shape of active site

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15
Q

Feedback Inhibition

A

Control mechanism
• Positive and Negative

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16
Q

Glycolysis

A

Catabolism of Glucose
• Embden-Meyerhof-Parnas pathway
• Substrate level phosphorylation

Net Gain
• Two ATP
• Two NADH
• Two pyruvate

Other pathways
•Entner-Doudoroff pathway
• Pentose phosphate pathway

17
Q

Transition Reaction

A

• Pyruvate -> Acetyl group
• Catalyzed by pyruvate dehydrogenase

18
Q

The Krebs Cycle

A

AKA: The Citric Acid Cycle
• Closed pathway

Net output per turn
• Two CO2
• Three NADH
• One FADH
• One ATP

Two turns per Glucose

19
Q

Electron Transport System

A

Oxidative phosphorylation
• Majority of ATP production

Location
• Cell membrane of prokaryotes
• Mitochondrial membrane of eukaryotes

Aerobic & Anaerobic

20
Q

Chemiosmosis

A

• Flow of hydrogen lons
• ATP synthase