Microbial Control Flashcards
the process of destroying all forms of microbial life.
Sterilization
An agent, usually a chemical that kills the grow forms but not necessarily the resistant spore forms of disease – producing microorganisms.
Disinfectant
is the process of destroying infectious.
Disinfection
A substance that opposes sepsis, to prevent the growth or action of microorganism or action microorganisms either by destroying or by inhibiting their growth and metabolism.
Antiseptics
An agent that reduces the microbial population to safe levels as judged by public health requirements.
Sanitizer
An agent that kills the growing forms but not necessarily the resistant spore forms of germs
Germicide (Microbicide)
An agent that kills bacteria, similarly the terms fungicide, virucide and sporicidal refers to agents that kill fungi, viruses and spores respectively.
Bactericide
A condition in which the growth of bacteria is prevented. Similarly, fungistatic describes an agent that stops the growth of fungi.
Bacteriostasis
One that interferes with the growth and metabolism of microbes.
Antimicrobial agent
Some antimicrobial agents are used to treat infections, and they called
chemotherapeutic agents
Methods of Microbial Control:
- Nature of the material to be treated
- Types of microorganisms
- Environmental conditions
To cite an extreme example ( a chemical agent used in disinfectant contaminated utensils might be quite unsatisfactory for application to the skin; It might do serious injury to the tissue cells.
Nature of the material to be treated
Chemical agents are not all equally effective against bacteria, fungi, viruses and other microorganisms.
Types of microorganisms
The factors temperature, pH, time, concentrations and presence of extraneous organic material, may all have a bearing on the rate and efficiency of antimicrobial action.
Environmental conditions
Temperatures below the minimum usually have a ____ action on microorganisms.
static
An agent that is static in action will inhibit the growth of microorganisms.
static
An agent that is cidal in action will kill microorganisms and viruses.
cidal
Temperatures above the maximum usually have a ____ action on microorganisms.
cidal
Keep in mind that when evaluating or choosing a method of controlling microorganisms, you
must consider the following factors which may influence antimicrobial activity:
- the concentration and kind of a chemical agent used;
- the intensity and nature of a physical agent used;
- the length of exposure to the agent;
- the temperature at which the agent is used;
- the number of microorganisms present;
- the organism itself; and
- the nature of the material bearing the microorganism
Vegetative microorganisms can generally be killed at temperatures from 50°C to 70°C with moist heat.
High Temperature
are very resistant to heat and extended exposure to much higher temperature is necessary for their destruction
Bacterial endospores
is generally more effective than dry heat for killing microorganisms because of its
ability to penetrate microbial cells.
Moist heat
Moist heat kills microorganisms by
denaturing their
proteins
employs steam under pressure.
Autoclaving