microbial cell Flashcards

1
Q

what is microbiology

A

“The study of micro-organisms, their physiology, biochemistry and genetics in single culture, in complex environments and their interactions with host organisms”

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2
Q

Name two main themes in microbiology.

A

Understanding basic life processes (using microbes as models for cellular processes).

Applying knowledge of microbes for human benefit (e.g., in medicine, agriculture, and industry).

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3
Q

What are microorganisms?

A

Life forms too small to be seen by the human eye. They are diverse in form and function, inhabit all environments supporting life, and can be single-celled, multicellular, or form complex structures.

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4
Q

When did the first cells appear on Earth?

A

Between 3.8 and 3.9 billion years ago.

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5
Q

What is the role of microbes in agriculture?

A

Positive roles: Nitrogen fixation, cellulose degradation in the rumen, and nutrient regeneration in soil and water.

Negative roles: Causing diseases in plants and animals.

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6
Q

What is the significance of bacterial cell walls?

A

Maintains cell shape.

Provides rigidity via peptidoglycan structure.

Differentiates Gram-positive and Gram-negative bacteria (via Gram staining).

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7
Q

What are the characteristics of Gram-positive and Gram-negative bacteria?

A

Gram-positive: Retain crystal violet stain (purple); thicker peptidoglycan layer.

Gram-negative: Do not retain crystal violet stain; counterstained pink with fuchsin; have an outer membrane with lipopolysaccharide (LPS).

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8
Q

What are bacterial endospores?

A

Dormant structures that persist for long periods, are dehydrated, and resistant to environmental stresses. They can germinate to form new vegetative cells.

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9
Q

What are the functions of the cytoplasmic membrane?Back:

A

Acts as a barrier separating the cytoplas

Facilitates energy production (e.g., via electron transport chain).

Aids in signal transduction and cell wall synthesis.

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10
Q

What are pili, and what is their function?

A

Small hair-like structures used for attachment to surfaces, other cells, or tissues. Specialized pili are involved in DNA transfer during bacterial conjugation.

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11
Q

How do bacterial flagella function?

A

Flagella are large hair-like structures that rotate to allow bacterial motility. They are powered by proton motive force and are used in processes like chemotaxis and phototaxis.

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12
Q

What are magnetosomes?

A

Magnetic iron oxide inclusions that allow cells to migrate along magnetic field lines. Applications include medicine, drug delivery, and bioremediation.

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13
Q

What is the significance of the human microbiome?

A

It includes all microorganisms residing on and in the human body, influencing health, immunity, and metabolism.

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14
Q

What are some industrial uses of microbes?

A

Pproduction of antibiotics, enzymes, chemicals, and biofuels. They are also used in bioremediation to clean up pollutants.

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15
Q

Why is peptidoglycan important in bacterial cells?

A

It provides rigidity to the cell wall, maintains cell shape, and is a target for antibiotic mechanisms of action

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16
Q

What distinguishes archaeal cell walls from bacterial cell walls?

A

Archaeal cell walls lack peptidoglycan and may contain pseudomurein. Their membranes are adapted to extreme environments and lack fatty acids, instead containing isoprenes.

17
Q

What is the evolutionary significance of rRNA?

A

Ribosomal RNA (rRNA) sequences are conserved and can be used to infer evolutionary relationships. Carl Woese used rRNA to establish the three-domain system (Bacteria, Archaea, Eukarya).