Microbe Flashcards

1
Q

E. COLI characteristics

A

GRAM - Bacilli, Lactose Fermentator (Beta-Galactose), Encapsulated, motile (explains Asc UTI’S), UREASE -

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2
Q

Where E.coli Grows

A

-Eosin Methylene Blue agar (black colonies)
-MaConkey

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3
Q

What bacteria Grows in Eosin methylene Blue agar

A

E.coli

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4
Q

E.Coli virulence Factors ass. (3)

A

**Fimbria **(cystitis and Pyelonephritis)
K capsule (meningitis and pneumonia)
LPS Endotoxin (septic shock)

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5
Q

Thrombocytopenia, Hemolytic anemia, Azotemia ass with Hamburger eating 1 day ago. .

A

STEC (Shiga Like Toxin producing E.coli). = Entero-Hemorargic E.coli (EHEC)
Schistocytes….

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6
Q

Why female get UTI more common

A

Shorter urethra+ closer to anal canal.

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7
Q

STECT (E.coli) special characteristic

A

DOSENT fermented SORBITOL

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8
Q

0157:H7

A

EHEC ,ass HUS E.coli

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9
Q

How do you differentiated between Staph Saprophyticus and Epidermais

A

Saprophytycus is NovoBiocin resistant (or negative sensative).

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10
Q

Travelers diahrrhea ass with?

A

-Entero-Toxigenic E.Coli
-Heat labile and Heat stable Enterotoxin–>inflamation of intestine–>**Watery diarrhea. **

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11
Q

Entero-Pathogenic E.Coli (EPEC)

A

-<2yr old, NO toxin produced-->causes Intestinal epi flattening by going intracellular and damaging cytoskeleton–>DECR absorption water and nutrients–>watery diarrhea.

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12
Q

Uropathogenic E.Coli (UPEC) virulence factors

A

-P Fimbriae-->helps in ascending throught kidney
-Type 1 Pilli–>helps in attachement on epitheleal cells.
-alpha and Beta Hemolysin–>lysis of cells in urinary tract.

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13
Q

What serotype ?

Dysuria,Incr urinary frequence and flank pain//On culture you see GRMA- bacilli with motile and is encapsulated.

A

Uropathogenic-E.Coli (UPEC)

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14
Q

Describe

A

-Image on right shows sensitivity towards novobiocin (Stap. Aureus and Epidermadis)
-Image on left has resistance.
-This test used to differentiate between staphyloccocus organisms.

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15
Q

-GRAM+ in clusters,coagulase -, Novobiocin resistant,urease +
-Ass sexually active female in her 20’s…

A

-Staph Saprophyticus.

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16
Q

Common risk factor for all microbes that are UREASE+.

A

-Urease + converts urea–>ammonia + CO2—>INCR pH (alkalanization)–>promotes formation of struvite stones (made up off ammonia+phosphate+magnesium)

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17
Q

Klebsiella,Staph. saprophyticus, Proteus mirabilis have in common?

A

-All are urease +.

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18
Q

-Priamary cause of UTI in females?
-Secondary

A

-E.COLI
-Staph saprophyticus.

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19
Q

Lactose fermentador and NOT urease +

A

E.coli

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20
Q

DIAGNOSIS and caharacteristics

A

-Proteus Mirabilis (Ripple=SWARMING)
-GRAM- Bacilli,urease+,aggresivly motile,POLYMORPHIC Hydrogen sulfate** NON-LACTOSE FERMENTADOR**–>differentiate between E.Coli.
-Ass with old people Hospitalized with catheter placement.

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21
Q

What microbes are polymorphic?

A

Proteus mirabilis and H.Influenza.

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22
Q

DIAGNOSIS

-Exposure to catheter forms “swarm cells” (100-1k flagella–>aggressive motile)–>moves along catheter until reaches epi.–>use Fimbriea to attach–>rel. Hemolysin.

A

Proteus Mirabilis

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23
Q

Triple Sugar Iron Test used for what?

A

-Used for diagnosis of Proteus mirabilis–>has Hydrogen Sulfate.

24
Q

Fishy odor?

A

-Proteus Mirabilis.

25
Q

Grape-like-odor?

A

Pseudomonas Aerginosa

26
Q

Where do you see?

A

-Urease+ microbes.
-Staghorn stones.

27
Q
A

Enterococcous species.

28
Q

Dianosis

A

Diapper rash with satellite cells
-Candida Albicans

29
Q

Diagnosis

A

Trematones (Schistisoma Hematobium)
-Transmited via water in infected snails.
-Penetrates skin.

30
Q

Identify

A

Right:Staghorn stone
Left:stuvite stone
-Both ass with urease+ organism.

31
Q

Ecthyma Gangrenosum ass…

A

Pseudomonas Aruginosa
-Rapidly progrresive necrotic cutaneous lesion.

32
Q

-GRAM-Bacilli,non-lactose fermentador, oxidase+
Mucoid colonizes that are blue-green.

A

-Psuedomonas Aeruginosa

33
Q

-GRAM- Bacilli non lactose fermentador ass with Pneumonia in patients with Cystic Fibrosis.

A

Pseudomonas Aeruginosa
-Mucopolysacharide capsule.

34
Q

P.Aeruginosa Virulenece Factors

A

-Phospholipase C (lysis membranes)
-Endotoxin (septic shock)
-Exotoxin A (EF2 inhibitor)

35
Q

P.Aeruginosa related diseases.

A

-Sepsis,otitis externa, pneumonia, UTI (catheter patients),ecthyma gangrenosum,IV drugs users

36
Q

What can we assume if you get hospital acquiered Pseudomonas Aeruginosa?

A

-Multi-drug resistant.

37
Q

-GRAM+ cocci in chains, Gamma Hemolytic, 6.5% NaCl solutin grows and PYR +

A

Enterococcous species (fecalis)

38
Q

Patient had a appendectomy last week and today presents with UTI.
-Most likely bacteria?

A

Enterrococus species.

39
Q

3 Infections associated with Enterococcous species

A

-UTI, Infective Endocarditis, Biliarytract infections.

40
Q

Candida Albicans charactristics and related immunosupressive states (4)?

A

-Dimorphic Fungi, psuedohyphae+ yeast 20C*
-Opportunistic infections (Diabetus,Inhaled corticosteroids, AIDS, chemotherapy,Children).

41
Q

Candida 3 mayor infections

A

-Vulvovaginitis, Diapper rash and Pseudomembranous candidiasis ( witish cheese apperance)

42
Q

Presentation of vulvovaginitis..

A

-Dysuria, Purulent discharge, Itching.

43
Q

Diagnsosis

A

-Candida Albicans

44
Q

What increases risk for Disseminated Candidiasis?

A

Neutropenia

45
Q

Aspergillosis

A

-Ass with Farmers (grain farmers),remodeling hosuses->Aspergilloma in TB,incr risk for Hepatocellular carcinoma, Invasive disease,Bronchioctasis.
-Acute angles and secrete conidia.
-People at risk are neutropenic

46
Q

Risk factor for candida UTI

A

Catheter placement–>biofilm production

47
Q

Describe Helminths…

A

-Worms that are multi-cellular.
-Nematodes,Tematones, Cestones.

48
Q

Cercaria

A

-Infective form of type of tematone (schistosoma hematobium)

49
Q

Schistosoma Hematobium related with secretion form where?

A

-Snails–>secretes cercaria–>penetrate human skin–>forms egg–>promote I.S to respond in a chronic manner.
-Dysuria,Hematuria, incr risk of squamous cell carcinoma of Badder,hepatosplenomegaly,granuloma formation.

50
Q

What 2 cytokines are important to know for Schistosoma Hematobium and why?

A

IL4 and IL13–> intense granuloma and fibrosis formation.

51
Q

Praziquantel??

A

Used for tematones infect
-Incr Ca++ permeability.

52
Q

Most comm honeymoon cystitis
-What test will contraindicate E.coli?

A

E.Coli>Staph saprophyticus
-Nitrate - will be staph. saprophyticus

53
Q

What antibiotics do you give in Hospital acquired Enterrococus UTI?
-Why?

A

-Vancomycin+Aminoglycosides-->due to being very resistant (comm antibiotics will not work).
-Enteroccocus species are the most resistant in streptoccocus.

54
Q

Klebsiella Pneumonie descriptons
-V.F

A

-GRAM - Rod, polysacharide capsule, urease +, non motile, mucoid colonizes, lactose fermentador, grows in MacConkeys agar

-capsule, Pilli, LPS, saderophores (need Iron )

55
Q

What does Maconckey Agar tells you and what does it have?

A

-If bacteria is lactose fermentador
-Has lactose + pH sensative.

56
Q

-Lobar Pneumonia ass with alcohol overuse –>aspiration–>lung abscess and patient with diabetus mellitus.

-Also causes UTI with struviate stones.

A

Klebsiella

57
Q

Organism that are urease +

A

P (Proteus)
Cryptoccocus
H.Pylori
Ureaplasma
Nocardia
Klebsiella
Staph Saprophyticus
Staph epidermis