Micro1 Flashcards
1
Q
what bugs do NOT gram stain well?
A
“These Rascals May Microscopically Lack Color”
Treponema - too thin Rickettsia - intracellular parasite Mycobacteria - acid fast Mycoplasma - no cell wall Legionella pneumophilia - intracellular Chlamydia - intracellular parasite, lacks muramic acid in cell wall
2
Q
Giemsa stain.
A
“Certain Bugs Really TRY my Patience”
Chlamydia Borrelia Rickettsiae Trypanosomes Plasmodium
3
Q
PAS (periodic acid-Schiff) stain.
A
Stains GLYCOGEN, mucopolysaccharides
Used to diagnose Whipple’s dz
-Tropheryma whipplei
“PASs the sugar”
4
Q
Ziehl-Neelsen (carbol fuchsin) stain.
A
Acid fast organisms - Nocardia. Mycobacterium
5
Q
India ink stain.
A
Cryptococus neoformans
*Mucicarmine can also be used to stain thick polysaccharide capsule RED
6
Q
Silver stain.
A
Fungi (i.e. Pneumocystis)
Legionella
H. pylori