Micro1 Flashcards

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1
Q

what bugs do NOT gram stain well?

A

“These Rascals May Microscopically Lack Color”

Treponema - too thin
Rickettsia - intracellular parasite
Mycobacteria - acid fast
Mycoplasma - no cell wall
Legionella pneumophilia - intracellular
Chlamydia - intracellular parasite, lacks muramic acid in cell wall
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2
Q

Giemsa stain.

A

“Certain Bugs Really TRY my Patience”

Chlamydia
Borrelia
Rickettsiae
Trypanosomes
Plasmodium
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3
Q

PAS (periodic acid-Schiff) stain.

A

Stains GLYCOGEN, mucopolysaccharides

Used to diagnose Whipple’s dz
-Tropheryma whipplei

“PASs the sugar”

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4
Q

Ziehl-Neelsen (carbol fuchsin) stain.

A

Acid fast organisms - Nocardia. Mycobacterium

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5
Q

India ink stain.

A

Cryptococus neoformans

*Mucicarmine can also be used to stain thick polysaccharide capsule RED

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6
Q

Silver stain.

A

Fungi (i.e. Pneumocystis)
Legionella
H. pylori

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