MICRO by Sir Jed Flashcards
He established the germ theory of disease and discovered MTB
a. Jon Snow
b. Robert Koch
c. Louis Pasteur
d. Antoine Van Leeuwenhoek
Robert Koch
An endoscope for intestinal imaging
a. semicritical material
b. Critical materials
c. Noncritical materials
d. NOTA
semicritical material
Antiseptic technique for blood culture
a. 70% alcohol
b. 70% alcohol then 2% iodine
c. 2% Iodine then 70% alcohol
d. Alcohol, iodine, alcohol
70% alcohol then 2% iodine
Used to sterilized HEPA filters in BSC
a. Ethylene oxide
b. Bleach
c. Moist heat
d. Formaldehyde vapor
Formaldehyde vapor
Most resistant microorganism
a. Prion
b. Bacillus
c. Mycobacteria
d. Lipid virus
Bacillus
- Order of Most Resistant to Least: Prions > Spore > mycobacteria > nonlipid virus > Fungi > bacteria >
lipid virus (susceptible to detergent)
Prion is not a microorganism
The ff. is a wrong practice in microbiology lab
a. Keeping shoulder-length hair
b. Wearing of conservative jewelry
c. Wearing crocs in the lab
d. Opening culture plate in BSC
Wearing crocs in the lab
No recirculation of air, total Exhaust via HEPA, BSC II
a. Type A1
b. Type AII
c. Type B1
d. Type B2
Type B2
- Type AI – 70% recirculated thru HEPA (A.22)
- Type AII/B3 – same as IIa, but it can filter toxic chem and radionucleotides
- Type B1 – 30% recirculated thru HEPA (a.22)
- Type B2 – no recirculation, total Exhaust via HEPA
Is the definitive lab reference for questions related to individual test
a. SOP manuals
b. Procedure
c. Reference book
d. NOTA
SOP manuals
instrument maintenance record should be retained
a. for 1 yr
b. for 2 yrs
c. for the life of the instrument
d. for 5 yrs
for the life of the instrument
checking media and reagents with specific organisms to determine whether expected results are obtained and documenting that the instrumentation meets all operating parameters before it is used on patient samples
a. Quality management
b. Quality assurance
c. Quality assessment
d. Quality control
Quality control
The ff. are part of quality assurance except
a. Preanalytical
b. Analytical
c. Post analytical
d. Accuracy and precision
Accuracy and precision
Incubator temperature should be checked
a. Weekly
b. Monthly
c. 4x/ month
d. Daily
Daily
lab must perform QC on all of the ff. media except
a. complex media
b. media made by the lab
c. Commercial media
d. media with a history of failure
Commercial media
- Commercial media are always retested by the manufacturer, but laboratory must obtain a statement of QC from the manufacturer of all media that the lab will not retest
Virus for QC stock should be stored
a. Indefinitely at -70oC
b. 1 yr on TSA
c. thawed and refrozen every 3 yrs
d. NOTA
Indefinitely at -70oC
Choice of collection for C. diphtheriae and N. meningitidis?
a. Sputum
b. Lumbar puncture
c. Nasopharynx
d. Blood
Nasopharynx
Amies, Stuart, or Cary–Blair are NOT suitable for the transport of swabs for culture of most
pathogens except __________
a. C. diphtheriae
b. N. gonorrhoeae
c. N. meningitidis
d. Enterobacteriaceae
N. gonorrhoeae
A patient who took barium should be deferred from submitting stool sample for
a. 4 days
b. 6 days
c. 3 days
d. 2 days
4 days
- 4 to 5 days
The two most common organisms isolated from skin and soft tissue infections/soft tissue abscess
a. S. aureus and C. albicans
b. S. aureus and S. pyogenes
c. S. aureus and E. coli
d. S. aureus and Enterococci
S. aureus and S. pyogenes
method of choice for recovery of anaerobic bacteria from a deep abscess
a. swab in the margin
b. aspirate in the center of lesion
c. aspirate in the margin of lesion
d. swab in the center of lesion
aspirate in the margin of lesion
Calcium Alginate is toxic to
a. Neisseria
b. Virus
c. Chlamydia
d. Aota
Virus
*HSV
The purpose of adding 0.025%–0.050% sodium polyanetholsulfonate (SPS) to nutrient broth
media for the collection of blood cultures
a. Inhibits phagocytosis and complement
b. Inhibits Neisseria and Anaerobes
c. Inhibits antibody
d. Inhibits clotting of blood
Inhibits phagocytosis and complement
This additive should never be used in Microbiology, except
a. Heparin
b. EDTA
c. Citrate
d. NOTA
Heparin
- Heparin = USED FOR VIRAL CULTURES and FOR ISOLATION OF MYCOBACTERIUM SPP.
FROM BLOOD
Specimen should be transported to the laboratory ideally within 30 MINUTES of collection,
preferably within ______
a. 1 hr
b. 2 hrs
c. 3 hrs
d. 4 hrs
2 hrs
The ff. is/are subject for rejection for culture except
a. Saliva
b. unrefrigerated urine more than 2 hours
c. Dried specimen
d. Bone marrow aspirate more than 4 hrs
Bone marrow aspirate more than 4 hrs
- Suboptimal samples FROM INVASIVE PROCEDURE should still be processed SPECIMENS IMPOSSIBLE TO RECOLLECT/From Invasive procedure: Bone marrow, spinal fluid, or surgery (Effusions not included but maybe can be part also)