BACTE 2 Flashcards
Growth slows down, there is a competition for food, temp rises, and waste begin to accumulate
a. Lag
b. Log
c. Stationary
d. Decline
Stationary
Lag – increase in size
Log – exponential growth
Decline – bacteria death
Which among the ff. will rule in a bacterial endotoxin
a. Enzymes and toxins
b. A polysaccharide
c. Released by living bacteria
d. Can be detected using blood of horse shoe crab
Can be detected using blood of horse shoe crab
A – exotoxin
B – it’s a lipopolysaccharide
C- exotoxin
True about Polysaccharide capsule
a. Inhibit phagocytosis, thus it is a chief determinant of phagocytosis
b. It is stained by india ink
c. It can be found in S. pneumoniae, S. agalactiae, and N. meningitides
d. A and C
A and C
Organic compound is the final electron acceptor
-Produces 2 ATP
-An anaerobic process
a. Fermentation
b. respiration
c. oxidation
d. a and c
Fermentation
In catalase test, 3% H 2 O 2 is converted to
a. CO2 and O2
b. O2 only
c. H2O
d. H2O and O2
H2O and O2
Positive reaction = bubbling or effervescence
The ff. are phenotypic characteristics of Micrococcus except
a. Does not ferment carbohydrate
b. Modified oxidase positive
c. Lysosome sensitive
d. Bacitracin Resistant
Bacitracin Resistant
it is bacitracin sensitive
The ff. are similarities of Staphylococcus and Streptococcus except
a. Cause multi -organ failure/TSS
b. Facultative anaerobe
c. Impetigo, folliculitis, and carbuncles
d. Cause clot production
Cause clot production
True about the principle of macrolide resistance (D test)
a. S.aureus is resistant to clindamycin
b. S. aureus is susceptible to Erythromycin
c. S. aureus becomes resistant to erythromycin when mixed with clindamycin
d. S. aureus becomes resistant to clindamycin when in synergy with erythromycin
S. aureus becomes resistant to clindamycin when in synergy with erythromycin
Erythromycin – resistant
Clindamycin – susceptible
Erythromycin + Clindamycin = Clindamycin resistance
Alpha-prime – small ring of __________ hemolysis which is surrounded by a wider ___ hemolysis
a. Complete, no
b. No, complete
c. Complete
d. No
No, complete
What is the principle of Neufeld Quellung test/method?
a. Complement activation by Ag-Ab complex
b. Biochemical reaction
c. Genetics
d. Capsular swelling
Complement activation by Ag-Ab complex
Complex media for LANCET/OVAL shape Streptococcus called S.pneumoniae?
a. Brain Heart infusion
b. Trypticase soy agar w/5% Sheep blood
c. Chocolate agar
d. AOTA
AOTA
What is the reagent in bile solubility test?
a. Bile
b. Sodium deoxycholate
c. ethylhydrocupreine hydrochloride
d. Hippurate and Ninhydrin
Sodium deoxycholate
To demonstrate streptolysin O, where should the technologist observe on SBA?
a. Stabbed part
b. Streaked part
c. Surface hemolysis
d. AOTA
Stabbed part
Streptolysin O is oxygen Labile, it will be neutralized in the presence of oxygen, therefore it won’t show activity in the SURFACE or Streak area
A throat culture was request by a physician, what is the most likely specmien to be isolated?
a. Staphylococcus
b. Streptococcus
c. Neisseria
d. AOTA
Streptococcus
This method is more reliable in identifying S. pyogenes?
a. Biochemical reaction
b. Culture characteristics
c. Serology
d. Neufeld Quellung
Serology
A-hemolytic, wet, mucoid, round, glistening, and dome-shaped appearance
a. 3 rd most common cause of otitis media
b. Principal cause of neonatal sepsis
c. Number 1 cause of food poisoning
d. Most common cause of adult lobar pneumonia
Most common cause of adult lobar pneumonia
A – M. catarrhalis
B – S. agalactiae
C – S.aureus
D – S.pneumoniae
Clot is the positive result of this test
a. Tube coagulase
b. Slide coagulase
c. Catalase
d. Bile solubility
Tube coagulase
B – clump
C –effervescence
D – clearing
Resistant to hydrolysis by the gastric and intestinal enzymes. The toxins, which are often found in milk products, are associated with pseudomembranous enterocolitis and toxic shock syndrome, and they may exacerbate the normal immune response, resulting in further tissue damage and systemic pathology
a. Heat-stable enterotoxin
b. Streptolysin O
c. Panton valentin leucocidin
d. Sphingomyelinase C
Heat-stable enterotoxin
This is associated to community acquired pneumonia
a. Gamma hemolysin
b. Beta hemolysin
c. Alpha hemolysin
d. Streptolysin
Gamma hemolysin
A - Assoc.to Panton Valentin Leukocidin:
True about NVS, except
a. Satellites Staphylococcus
b. Gram variable
c. Add Vit B6 in culture plate for growth
d. NOTA
NOTA
True about Neisseria except
a. Kidney/coffee bean shaped Diplococci
b. Capnophile, Oxidase, Catalase positive
c. Fastidious, sensitive to cold, dry, antiseptic, and disinfectant
d. NOTA
NOTA
The ff. can be a sample for N.gonorrhoeae except
a. Blood
b. Rectal, eye, and throat swab
c. Endocervix and urethral swab
d. Nasopharyngeal
Nasopharyngeal
This is an alternative for anaerobic jar because it reduces oxygen and produces CO2
a. Candle
b. Candle jar
c. JEMBEC
d. Amies
Candle jar
The ff. techniques can be done for oxidase test except?
a. Drop the reagent directly to the colony
b. Rub the colony on paper and add the reagent
c. Add the reagent to the paper and rub the colony on it
d. Rub the colony on the reagent
Rub the colony on the reagent
Penicillin Test: Differentiate some Gram negative rods from Gram Negative Diplococci, after the application of penicillin on the medium, where should be get the sample?
a. Nearest the disk
b. Edge of the ZOI
c. Outside of the ZOI
d. Between the ZOI and the disk
Edge of the ZOI
True about M. catarrhalis
a. Lower Respiratory tract flora
b. Does not grow on blood and chocolate agar
c. Does not ferment carbohydrate
d. DNAse negative
Does not ferment carbohydrate
A – upper resp tract
B - grows on blood and choc
C – asaccharolytic
D – DNAse positive
Allowed sample for N. meningitides except
a. CSF
b. Joint fluid
c. Blood
d. NOTA
NOTA
For N.meningitidis, nasopharyngeal swabs are cultured to detect?
a. Reservoirs
b. Vectors
c. Coinfection
d. Host
Reservoirs
In bacteria causing Waterhouse Friderichsen, what should we look in direct microscopic examination of CSF?
a. Lymphocytes
b. Intracellular diplococci
c. Extracellular diplococci
d. B and C
B and C
Superoxol is one of the methods to detect sexually transmitted diplococci, what reagent is use for this test?
a. 3% H202
b. 30% H202
c. Both a and b
d. DNA conjugated with methyl green
30% H202
