BACTE 3 Flashcards
Gram variable aerobic, spore-negative catalase positive bacilli causing neonatal abortion?
a. Can be grown using cold enrichment
b. Grows in Cystine tellurite agar
c. Test tube brush pattern in soft gelatin agar
d. Shows dot or pitting on SBA
Can be grown using cold enrichment
Molar tooth appearance is observed in these bacteria
a. Actinomadura
b. Actinomyces
c. Both a and b
d. Nocardia
Both a and b
Gram positive aerobic bacilli, spore positive, lecithinase positive, non motile, non hemolytic, and its colony has GROUND GLASS APPEARANCE?
a. Bacteria causing gas gangrene
b. Has Swirling projection under the microscope
c. Amine whiff test positive
d. Musty basement odor
Has Swirling projection under the microscope
The ff. can be used to differentiate Nocardia and Actinomadura
a. Sulfur granules
b. Latter is Cellobilose and xylose positive
c. Former is partial acid fast
d. B and C
B and C
The in-vitro toxin test of C.diphtheriae is based on
a. The reaction of antitoxin and bacterial toxin
b. Development of purulent conjunctivitis of an animal
c. Metabolism of lecithin causing a reduced opacity
d. Addition of 10% KOH to a discharge sample
The reaction of antitoxin and bacterial toxin
B – Ocular Anton by Listeria
C – Lecithinase by C.perfringens
D – Amine whiff test for G. vaginalis
Gram positive, anaerobic spore-forming bacilli that only produce enterotoxin?
a. Bacteria causing risus sardonicus
b. Lipase positive found in homemade canned vegetables
c. Lecithinase and Nagler Reaction positive
d. Terminal spore bacteria
Lecithinase and Nagler Reaction positive
Bacteria that ENTEROTOXIN only: C. perfringens and C.difficile
C. tetani – neurotoxin
C. botulinum – neurotoxin and enterotoxin
A – C. tetani
B- C. botulinum
C – C.perfringens
D – C. tetani
What is the expected reaction of an isolate, which is suspected to be B.anthracis, from the ff. tests?
i. Penicillin
ii. Fermentation of salicin
iii. Gelatin Hydrolysis
a. Susceptible, negative, negative
b. Resistant, positive, positive
c. Susceptible, negative, positive
d. Resistant, positive, negative
Susceptible, negative, negative
Foul odor is emitted after the addition of 10% KOH to vaginal discharge
a. Must have overgrowth of Doderlein bacilli
b. Vaginal pH must be lower than 4.5
c. Hemolytic on Rabbit blood but not sheep blood
d. Check for the presence of WBCs surrounded by gram positive bacilli
Hemolytic on Rabbit blood but not sheep blood
Nagler reaction is based on ___________ of Lecithinase by C.perfringens
a. Neutralization
b. Precipitation
c. Production
d. Agglutination
Neutralization
most common isolated species of Bacillus in opportunistic infections such as bacteremia, post traumatic infections of the eye, and endocarditis?
a. B.subtilis
b. B. anthracis
c. B. cereus
d. B. thuringiensis
B. cereus
A – eye infection among heroin addicts
Facultative intracellular pathogen first identified by PCR from a duodenal biopsy?
a. Rhodococcus
b. T.whipplei
c. Gordonia
d. Tsukamurella
T.whipplei
Nocardia can be cultured in the ff.
a. Modified Thayer martin
b. BCYE
c. SDA
d. AOTA
AOTA
Skin test for B.anthracis
a. Schick
b. Francis
c. Ascoli
d. Dick
Ascoli
A – Diphtheria
B- S. pneumonia
D- scarlet by s. pyogenes
Gray black colonies and pleomorphic palisading (V and L) shape in gram staining?
a. E. rhusiopathiae
b. B. cereus
c. B. anthracis
d. C. diphtheriae
C. diphtheriae
Reverse CAMP positive?
a. A. haemolyticum
b. C. botulinum
c. Both a and b
d. B. anthracis
A. haemolyticum
Reverse CAMP positive: A. haemolyticum and C. perfringens
True about anaerobes
a. Infects mucosal surface
b. Produce of foul odor
c. Produce sulfur granules
d. AOTA
AOTA
A newborn girl becomes febrile and will not feed about an hour after birth. A gram-positive rod is recovered from blood cultures from the newborn. The isolate has the characteristics listed. What is the most likely identity of the isolate?
Weakly β-hemolytic on SBA
Gram-positive bacilli, no spores observed
Catalase-positive
Hydrogen-sulfide-negative
Motile at room temperature
a.Erysipelothrix rhusiopathiae
b. Listeria monocytogenes
c. Corynebacterium ureilyticum
d. Gardnerella vaginalis
Listeria monocytogenes
A 57-year-old man from New York City presents to the emergency
department with diarrhea, arthralgia, abdominal pain,
malabsorption, and weight loss of 10 lb over the last month. A
duodenal biopsy is performed, but no infectious agent is recovered
on culture media. However, on Gram stain, a gram-positive
rod is observed in macrophages in the biopsy tissue. The organism
is identified by 16S rRNA gene sequencing. What is the most
likely identity of this organism?
a. Bacillus anthracis
b. Nocardia asteroides
c. Tropheryma whipplei
d. Erysipelothrix rhusiopathiae
Tropheryma whipplei
A 17-year-old boy presented to an emergency department with a history of multiple episodes of febrile pharyngitis followed in 10 to 14 days with extensive desquamation of his hands and feet. The reoccurrences have followed several courses of antimicrobial therapy including amoxicillin and cephalosporins. Rapid group A streptococci screens and cultures have been consistently negative for Streptococcus pyogenes. A specimen with a request for an alternative agent was submitted to a reference laboratory. The results listed were observed. The patient was subsequently treated with erythromycin, and he recovered. What was the etiologic agent?
SBA: dry, wrinkled, slightly hemolytic colony, which at 48 hours is a dark spot sunken in the agar
Catalase-negative
Nitrate-negative
Reverse-CAMP-test-positive
a. Corynebacterium diphtheriae
b. Arcanobacterium haemolyticum
c. Listeria monocytogenes
d. Rhodococcus equi
Arcanobacterium haemolyticum
A commercial fisherman who had red sores on his hands was seen by his physician. Biopsy and culture of one of the lesions was performed. The culture grew an organism with the characteristics listed. What is the most likely organism?
Nonhemolytic on SBA
Gram-positive bacilli, no spores observed
Catalase-negative
Hydrogen-sulfide-production-positive
Growth in gelatin resembled a test-tube brush
a. Rhodococcus equi
b. Listeria monocytogenes
c. Lactobacillus acidophilus
d. Erysipelothrix rhusiopathiae
Erysipelothrix rhusiopathiae
Corynebacterium species often appear as ______________ on Gram stain.
a. Pleomorphic gram-positive club-shaped bacilli that appear in palisades or in V and L formations
b. Branching gram-positive bacilli that appear as fine, intertwining, delicate filaments
c. Short, thin gram-positive bacilli that appear in chains
d. Large square-ended, gram-positive or gram-variable bacilli in chains where the ends of the single cells fit snugly together
Pleomorphic gram-positive club-shaped bacilli that appear in palisades or in V and L formations
Bacillus cereus is most noted for causing:
a. Food poisoning
b. Meningitis
c. Sexually transmitted disease
d. Urinary tract infections
Food poisoning
An isolate with the appropriate colony and microscopic morphology
may be suspected to be Bacillus anthracis if it is:
a. β-Hemolytic on SBA
b. Nonmotile
c. Catalase-negative
d. Gram-negative, non–spore forming
Nonmotile
An aerobic, gram-positive, spore-forming bacillus was isolated from raw vegetables that were associated with an outbreak of gastroenteritis. The organism produced β-hemolysis, was catalase-positive, and was motile. The most likely organism is:
a. Bacillus anthracis
b. Nocardia asteroides
c. Bacillus cereus
d. Tsukamurella spp
Bacillus cereus
Some anaerobes are particularly susceptible to oxygen because they lack the enzyme:
a. Amylase
b. β-Lactamase
c. Superoxide dismutase
d. Glucose-6-phosphate dehydrogenase
Superoxide dismutase
Which of the following specimens would be unacceptable for anaerobic culture?
a. Aspirated pus
b. Cerebrospinal fluid
c. Tissue from biopsy
d. Urethral swab
Urethral swab
A gram-positive bacillus was isolated from a wound specimen
and had the following characteristics: double zone of β-hemolysis,
lecithinase-positive, lipase-negative, indole-negative. What is the most likely identification of this organism?
a. Clostridium perfringens
b. Clostridium ramosum
c. Clostridium septicum
d. Clostridium tetani
Clostridium perfringens
An organism that can live and grow in reduced concentrations of oxygen but prefers an anaerobic environment is known as a(n):
a. Capnophile
b. Obligate anaerobe
c. Facultative anaerobe
d. Aerotolerant anaerobe
Aerotolerant anaerobe
Large gram-positive spore-forming rods growing on blood agar as large, raised, β-hemolytic colonies that spread and appear as frosted green-gray glass are most likely:
A. Pseudomonas spp.
B. Bacillus spp.
C. Corynebacterium spp.
D. Listeria spp
Bacillus spp.
The only spore former listed is the Bacillus spp., which grow as large, spreading colonies on blood
agar plates. Pseudomonas spp. are gram-negative rods; Corynebacterium spp. appear as small, very dry
colonies on BAP; Listeria spp. appear as very small β-hemolytic colonies on BAP, resembling
Streptococcus species
Which is the specimen of choice for proof of food poisoning by Bacillus cereus?
A. Sputum
B. Blood
C. Stool
D. Food
Food
