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BACTE 3 Flashcards

1
Q

Gram variable aerobic, spore-negative catalase positive bacilli causing neonatal abortion?

a. Can be grown using cold enrichment
b. Grows in Cystine tellurite agar
c. Test tube brush pattern in soft gelatin agar
d. Shows dot or pitting on SBA

A

Can be grown using cold enrichment

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2
Q

Molar tooth appearance is observed in these bacteria

a. Actinomadura
b. Actinomyces
c. Both a and b
d. Nocardia

A

Both a and b

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3
Q

Gram positive aerobic bacilli, spore positive, lecithinase positive, non motile, non hemolytic, and its colony has GROUND GLASS APPEARANCE?

a. Bacteria causing gas gangrene
b. Has Swirling projection under the microscope
c. Amine whiff test positive
d. Musty basement odor

A

Has Swirling projection under the microscope

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4
Q

The ff. can be used to differentiate Nocardia and Actinomadura

a. Sulfur granules
b. Latter is Cellobilose and xylose positive
c. Former is partial acid fast
d. B and C

A

B and C

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5
Q

The in-vitro toxin test of C.diphtheriae is based on

a. The reaction of antitoxin and bacterial toxin
b. Development of purulent conjunctivitis of an animal
c. Metabolism of lecithin causing a reduced opacity
d. Addition of 10% KOH to a discharge sample

A

The reaction of antitoxin and bacterial toxin

B – Ocular Anton by Listeria
C – Lecithinase by C.perfringens
D – Amine whiff test for G. vaginalis

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6
Q

Gram positive, anaerobic spore-forming bacilli that only produce enterotoxin?

a. Bacteria causing risus sardonicus
b. Lipase positive found in homemade canned vegetables
c. Lecithinase and Nagler Reaction positive
d. Terminal spore bacteria

A

Lecithinase and Nagler Reaction positive

Bacteria that ENTEROTOXIN only: C. perfringens and C.difficile
C. tetani – neurotoxin
C. botulinum – neurotoxin and enterotoxin

A – C. tetani
B- C. botulinum
C – C.perfringens
D – C. tetani

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7
Q

What is the expected reaction of an isolate, which is suspected to be B.anthracis, from the ff. tests?

i. Penicillin
ii. Fermentation of salicin
iii. Gelatin Hydrolysis

a. Susceptible, negative, negative
b. Resistant, positive, positive
c. Susceptible, negative, positive
d. Resistant, positive, negative

A

Susceptible, negative, negative

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8
Q

Foul odor is emitted after the addition of 10% KOH to vaginal discharge

a. Must have overgrowth of Doderlein bacilli
b. Vaginal pH must be lower than 4.5
c. Hemolytic on Rabbit blood but not sheep blood
d. Check for the presence of WBCs surrounded by gram positive bacilli

A

Hemolytic on Rabbit blood but not sheep blood

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9
Q

Nagler reaction is based on ___________ of Lecithinase by C.perfringens

a. Neutralization
b. Precipitation
c. Production
d. Agglutination

A

Neutralization

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10
Q

most common isolated species of Bacillus in opportunistic infections such as bacteremia, post traumatic infections of the eye, and endocarditis?

a. B.subtilis
b. B. anthracis
c. B. cereus
d. B. thuringiensis

A

B. cereus

A – eye infection among heroin addicts

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11
Q

Facultative intracellular pathogen first identified by PCR from a duodenal biopsy?

a. Rhodococcus
b. T.whipplei
c. Gordonia
d. Tsukamurella

A

T.whipplei

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12
Q

Nocardia can be cultured in the ff.

a. Modified Thayer martin
b. BCYE
c. SDA
d. AOTA

A

AOTA

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13
Q

Skin test for B.anthracis

a. Schick
b. Francis
c. Ascoli
d. Dick

A

Ascoli

A – Diphtheria
B- S. pneumonia
D- scarlet by s. pyogenes

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14
Q

Gray black colonies and pleomorphic palisading (V and L) shape in gram staining?

a. E. rhusiopathiae
b. B. cereus
c. B. anthracis
d. C. diphtheriae

A

C. diphtheriae

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15
Q

Reverse CAMP positive?

a. A. haemolyticum
b. C. botulinum
c. Both a and b
d. B. anthracis

A

A. haemolyticum

Reverse CAMP positive: A. haemolyticum and C. perfringens

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16
Q

True about anaerobes

a. Infects mucosal surface
b. Produce of foul odor
c. Produce sulfur granules
d. AOTA

A

AOTA

17
Q

A newborn girl becomes febrile and will not feed about an hour after birth. A gram-positive rod is recovered from blood cultures from the newborn. The isolate has the characteristics listed. What is the most likely identity of the isolate?

Weakly β-hemolytic on SBA

Gram-positive bacilli, no spores observed

Catalase-positive

Hydrogen-sulfide-negative

Motile at room temperature

a.Erysipelothrix rhusiopathiae
b. Listeria monocytogenes
c. Corynebacterium ureilyticum
d. Gardnerella vaginalis

A

Listeria monocytogenes

18
Q

A 57-year-old man from New York City presents to the emergency
department with diarrhea, arthralgia, abdominal pain,
malabsorption, and weight loss of 10 lb over the last month. A
duodenal biopsy is performed, but no infectious agent is recovered
on culture media. However, on Gram stain, a gram-positive
rod is observed in macrophages in the biopsy tissue. The organism
is identified by 16S rRNA gene sequencing. What is the most
likely identity of this organism?

a. Bacillus anthracis
b. Nocardia asteroides
c. Tropheryma whipplei
d. Erysipelothrix rhusiopathiae

A

Tropheryma whipplei

19
Q

A 17-year-old boy presented to an emergency department with a history of multiple episodes of febrile pharyngitis followed in 10 to 14 days with extensive desquamation of his hands and feet. The reoccurrences have followed several courses of antimicrobial therapy including amoxicillin and cephalosporins. Rapid group A streptococci screens and cultures have been consistently negative for Streptococcus pyogenes. A specimen with a request for an alternative agent was submitted to a reference laboratory. The results listed were observed. The patient was subsequently treated with erythromycin, and he recovered. What was the etiologic agent?

SBA: dry, wrinkled, slightly hemolytic colony, which at 48 hours is a dark spot sunken in the agar

Catalase-negative

Nitrate-negative

Reverse-CAMP-test-positive

a. Corynebacterium diphtheriae
b. Arcanobacterium haemolyticum
c. Listeria monocytogenes
d. Rhodococcus equi

A

Arcanobacterium haemolyticum

20
Q

A commercial fisherman who had red sores on his hands was seen by his physician. Biopsy and culture of one of the lesions was performed. The culture grew an organism with the characteristics listed. What is the most likely organism?

Nonhemolytic on SBA

Gram-positive bacilli, no spores observed

Catalase-negative

Hydrogen-sulfide-production-positive

Growth in gelatin resembled a test-tube brush

a. Rhodococcus equi
b. Listeria monocytogenes
c. Lactobacillus acidophilus
d. Erysipelothrix rhusiopathiae

A

Erysipelothrix rhusiopathiae

21
Q

Corynebacterium species often appear as ______________ on Gram stain.

a. Pleomorphic gram-positive club-shaped bacilli that appear in palisades or in V and L formations
b. Branching gram-positive bacilli that appear as fine, intertwining, delicate filaments
c. Short, thin gram-positive bacilli that appear in chains
d. Large square-ended, gram-positive or gram-variable bacilli in chains where the ends of the single cells fit snugly together

A

Pleomorphic gram-positive club-shaped bacilli that appear in palisades or in V and L formations

22
Q

Bacillus cereus is most noted for causing:

a. Food poisoning
b. Meningitis
c. Sexually transmitted disease
d. Urinary tract infections

A

Food poisoning

23
Q

An isolate with the appropriate colony and microscopic morphology
may be suspected to be Bacillus anthracis if it is:

a. β-Hemolytic on SBA
b. Nonmotile
c. Catalase-negative
d. Gram-negative, non–spore forming

A

Nonmotile

24
Q

An aerobic, gram-positive, spore-forming bacillus was isolated from raw vegetables that were associated with an outbreak of gastroenteritis. The organism produced β-hemolysis, was catalase-positive, and was motile. The most likely organism is:

a. Bacillus anthracis
b. Nocardia asteroides
c. Bacillus cereus
d. Tsukamurella spp

A

Bacillus cereus

25
Q

Some anaerobes are particularly susceptible to oxygen because they lack the enzyme:

a. Amylase
b. β-Lactamase
c. Superoxide dismutase
d. Glucose-6-phosphate dehydrogenase

A

Superoxide dismutase

26
Q

Which of the following specimens would be unacceptable for anaerobic culture?

a. Aspirated pus
b. Cerebrospinal fluid
c. Tissue from biopsy
d. Urethral swab

A

Urethral swab

27
Q

A gram-positive bacillus was isolated from a wound specimen

and had the following characteristics: double zone of β-hemolysis,

lecithinase-positive, lipase-negative, indole-negative. What is the most likely identification of this organism?

a. Clostridium perfringens
b. Clostridium ramosum
c. Clostridium septicum
d. Clostridium tetani

A

Clostridium perfringens

28
Q

An organism that can live and grow in reduced concentrations of oxygen but prefers an anaerobic environment is known as a(n):

a. Capnophile
b. Obligate anaerobe
c. Facultative anaerobe
d. Aerotolerant anaerobe

A

Aerotolerant anaerobe

29
Q

Large gram-positive spore-forming rods growing on blood agar as large, raised, β-hemolytic colonies that spread and appear as frosted green-gray glass are most likely:

A. Pseudomonas spp.
B. Bacillus spp.
C. Corynebacterium spp.
D. Listeria spp

A

Bacillus spp.

The only spore former listed is the Bacillus spp., which grow as large, spreading colonies on blood
agar plates. Pseudomonas spp. are gram-negative rods; Corynebacterium spp. appear as small, very dry
colonies on BAP; Listeria spp. appear as very small β-hemolytic colonies on BAP, resembling
Streptococcus species

30
Q

Which is the specimen of choice for proof of food poisoning by Bacillus cereus?

A. Sputum
B. Blood
C. Stool
D. Food

A

Food

MICRO (7 decks)

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