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MHC Flashcards

1
Q

HLA-B27

A

Ankylosing spondylitis

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2
Q

HLA-A29

A

Birdshot retinopathy

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3
Q

HLA-DR3, HLA-DR5, HLA-DR7

A

Celiac Disease

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4
Q

HLA-DR3

A

Graves’ Disease

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5
Q

HLA-DR2

A

Narcolepsy & Multiple Schlerosis

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6
Q

HLA-DR4

A

Rheumatoid Arthritis

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7
Q

HLA-DQ8, HLA-DQ2, HLA-DR3, HLA-DR4

A

Type 1 diabetes mellitus

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8
Q

Non Classical Class 1

A

HLA-E, HLA-F, HLA-G

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9
Q

Non Classical Class II

A

HLA-DM, HLA-DN, HLA-DO

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10
Q

Major antigen source of Endocytic Pathway

A

Extracellular & Membrane Proteins (host & foreign)

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11
Q

Processing Machinery for Endocytic Pathway

A

Lysosomal Enzymes

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12
Q

Cell Types where active

A

Professional APCs

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13
Q

MHC Binding site of antigen in Endocytic Pathway

A

Endocytic vesicles, prelysosomes

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14
Q

in Antigen pathway, the Endocytic Pathway is utilized in:

A

Class II

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15
Q

(Antigen Pathway) Endocytic Pathway is presents to:

A

CD4+ (helper) T Cells

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16
Q

Peptide Binding Site of Class I

A

a1 & a2

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17
Q

MHC CLASS I MOLECULES:

A

HLA-A, HLA-B,HLA-C

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18
Q

CLASS II MOLECULES:

A

HLA-DR, HLA-DQ, HLA-DP

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19
Q

Peptide Binding Site of Class II

A

a1 & b1

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20
Q

How many amino acids are needed in Class I?

A

8-10 amino acids

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21
Q

It is similar to the constants regions found in the immunoglobulin molecules.

A

a3 & B2 region

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22
Q

It reacts with CD8+ on cytotoxic T cells.

A

a3

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23
Q

Where do we found the Class II on antigen-presenting cells?

A

B cells, Macrophages, Monocytes,dendritic cells

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24
Q

molecular weight of alpha chain in Class II?

25
Molecular weight of bets chain in Class II
27kD
26
alleles in HLA-A, HLA-B, HLA-C?
HLA-A= 580 alleles HLA-B= 921 alleles HLA-C= 312 alleles
27
It contacts sequences in the B2 domain
CD4+
28
How many amino acid does Class II peptide needs?
9-20 amino acids
29
Rabbit serum Incubation
RT: 60 mins
30
battery of antisera known HLA Specificity
RT: 30 mins
31
HLA Phenotyping
Serological techniques
32
HLA Genotyping
Molecular Methods
33
3 Histocompatibility testing
1. Tissue testing 2. Antibody Screening 3. Tissue Matching/crossmatching
34
Anticoagulated whole blood is overlaid to:
-Ficoll-Hypaque reagent -then centrifuge
35
Characterized antibodies within WBCs
Dausset
36
Use of T Lymphocytes
Class I (HLA-A, -B, -C)
37
It use B lymphocytes
Class II (HLA-DR, HLA-DQ, HLA-DP)
38
3 B Lymphocytes separation:
1. Nylon Wool separation 2. Use of immunomagnetic beads 3. Fluorescent labeling ( Use of FITC)
39
FITC means:
Fluorescein Isothiocyanate
40
B cells adhere, physically remove
Nylon wool separation
41
It bounds to certain Antibody
Use of immunomagnetic beads
42
It binds to immunoglobulin
Fluorescein Isothiocyanate/ fluorescent labeling
43
Women with several pregnancy
Multiparous Women
44
Exposed to several HLA
px who received multiple transfusions (Platelets&WBCs)
45
what Dye is used in Complement-dependent lymphocytotoxicity
Trypan Blue dye (eosin Y)
46
True/False: Probability that any two individuals will express the same MHC molecule is very high.
False (very low)
47
Interpretation of 0-10% Dead Lymphocyte
Negative
48
Score & Interpretation of 11-20% dead Lymphocyte
2, Doubtful positive
49
It has a score 4 and Weak positive interpretation
21-50%
50
What is the score and % dead lymphocyte of the Positive interpretation?
6, 51-80%
51
What is the interpretation of 81-100% Dead Lymphocytes?
Strong positive
52
What PCR Based Technique is: PCR- amplification of a chosen sequence using primers flanking the sequence; The amplified DNA is immobilized in membrane
Sequence Specific Oligonucleotides
53
Oligonucleotide primer are designed to obtain amplification of specific alleles of group or alleles
Sequence-Specific Primers
54
The Sequence-Specific Primers are detected by:
Agarose Gel Electrophoresis & Transillumination
55
2 Methods of Sequence Based Typing
-Sanger-based DNA sequencing -Next generation DNA sequencing
56
4 types of Grafts
1. Autograft 2. Syngraft 3. Allograft (Homograft) 4. Xenograft (Heterograft)
57
What are the Tissues used in Transplantation?
-Kidney -Heart -Cornea -Lung -Skin -Bone Marrow
58
2 types of Graft-versus-Host Disease
-Acute GVHD -Chronic GVHD

IS (4 decks)

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