MGD3+4 Flashcards
How does oxygen binding affect Fe?
Fe moves into the plane of the ring of haem. This is a small conformational change that exposes the other haem groups more so binding to oxygen becomes easier.
What does the myoglobin-oxygen curve look like?
Hyperbolic
Define cooperative binding of oxygen
The binding of one oxygen molecule to haemoglobin promotes the binding of subsequent molecules of oxygen.
Why is the Bohr effect important?
It ensures the delivery of oxygen is coupled to the demand for oxygen.
What are thalassaemias?
A group of genetic disorders where there is an imbalance between the number of alpha and beta globin chains.
Which thalassaemia is onset before birth?
Alpha thalassaemia
Which chains (alpha or beta) can form stable tetramers alone?
Beta chains
What is the disadvantage to a haemoglobin made up of just beta chains?
It has an increased affinity for oxygen meaning less oxygen would be released in the tissues.
What is the precursor to NAD and NADP?
Vitamin B3
Define transition state
A high energy intermediates that lies between the reactants and the products.
Name the features of enzymes
highly specific unchanged after the reaction do not affect position of equilibrium increase rate of reaction usually proteins may require cofactors
What shape does the Michaelis-Menten equation predict a plot to be?
Rectangular hyperbola
What is 1 unit in terms of enzymes?
The amount of enzyme that converts 1 micromole of product per minute under standard conditions.
What is a haem group made of?
A central iron (Fe) atom bound to 4 nitrogen atoms and a histidine. This leaves on space for oxygen to bind to the iron.
