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Extra Stage 2 > MFD SBA > Flashcards

MFD SBA Flashcards

1
Q

Species highly correlated with BANA test -

A

P. Gingivalis

other red complex too

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2
Q

Constituent of MacConkey inhibits growth of gram positive bacteria -

A

bile salts

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3
Q

Synder test yellow result; gram positive rod

A

lactobacillus

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4
Q

pathogenicity of Staphylococcus aureus -

A

coagulase, hyaluronidase, deoxyribonuclease, exotoxins, superantigens; beta lactamases

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5
Q

Dental hand piece decontamination

A

autoclave

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6
Q

Which bacterial component causes an acute inflammatory reaction

A

LPS

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7
Q

Which bacteria is beta haemolytic on blood agar

A

S. pyogenes, P. gingivalis,

S. aureus

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8
Q

Which bacteria is alpha haemolytic on blood agar

A

oral streptococci

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9
Q

Which bacteria is gamma haemolytic on blood aga

A

staph

s. epidermidis

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10
Q

What factors contrinute to the pathogenicity of aggregatibacter actinomuycetemomitans:

A

Adhesins, bacteriocins, invasins

Trypsin like Proteases

Surface antigens

Heat shock proteins

Antimicrobial resistance

Localised aggressive PD

Leukotoxins which kill monocytes and neutrophils

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11
Q

Which bacteria is a spirochete

A

T. Denticola

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12
Q

What mechanism moves drugs from the blood to tissues?

A

distribution

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13
Q

MSB agar is to isolate which bacteria?

A

contains bacitracin which allows strep mutant and sobrinus to grow

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14
Q

Aetiological factor for carie

A

S. mutans

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15
Q

Infection control

A

cross infection control, hand washing, waste disposal (separate clinical and domestic), vaccination, medical history

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16
Q

What is the route of transmission for HBV?

A

Blood

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17
Q

Name a spore forming bacteria?

A

Clostridium difficile
c tetani
c botulinum

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18
Q

What is the definition of a microbiome?

A

total Mo in a particular environment

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19
Q

What protein structure is a factor for pathogenicity for C.tetani?

A

Neurotoxin

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20
Q

What protein structure is a factor for pathogenicity for N. gonorrhoea?

A

OP surface protein

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21
Q

What determines critical pH of saliva?

A

Peoples saliva composition varies so they therefore have different buffering capacitities

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22
Q

Name the gram+ rod in synder in dental plaque

A

Lactobacillus

23
Q

What polysaccharide is formed by strep and what mutans required a primer?

A

Fructans and glucans

glucans need primer except -
primer indépendant GTF which was called GTFSi ( comprise of )α-1,3 and α-1,6 bonds E.g. the GTF of S. gordonii

24
Q

1)What salivary component inhibits the growth of bacteria by binding to enzyme co-factor?

A

Lactoferrin

25
Q

How is drug resistance developed between bacteria?

A

Conjugation

26
Q

Mechanisms that cause penicillin resistance in bacteria?

A

Beta lactamase production

27
Q

In muscarinic stimulation PIP activates what membrane bound protein causing moderate amount of protein secretion?

A

DAG

28
Q

1)What salivary factor determines critical pH?

A

Ca2+ and PO4 concentration.

29
Q

What component of the secretory cell membrane lowers Na in the cell?

A

Na/K ATPase

30
Q

What contributes to the pathogenicity of s. aureus?

A

Exo/enterotoxin

31
Q

Why is CLED used for urine?

A

Electrolyte deficiency prevents swarming of proteus. Yellow = E-coli, Blue = Proteus.

32
Q

2)What component of MaConkey agar is inhibitory to gram -ve bacteria?

A

Sodium deoxycholate, bile salts . Selects for gram –ve bacteria.

33
Q

What is a product of tryptophan metabolism?

A

Skatole

34
Q

2) What bacteria would be present in a positive BANA test?

A

P. gingivalis, Treponema denticola, Tannerella forsythia

35
Q

What factor contributes to the pathogenicity of actinomyces actinomycetemcomitans?

A

aggressive periodontitis - the bacteria secretes leukotoxins which kill monocytes and neutrophils. Adhesins/invasins, proteases, antimicrobial resistance

36
Q

What drug stimulates the release of insulin?

A

Sulphonylureas (e.g. glibenclamide

37
Q

What type of reaction changes the ketone group to a hydroxyl group on warfarin?

A

Phase 1 reaction.

38
Q

What bacteria is alpha haemolytic on blood agar?

A

Oral streptococci

39
Q

What bacteria is gamma haemolytic on blood agar?

A

Staphylococcus species (s. epidermidis)

40
Q

A deficiency in what causes angular stomatitis?

A

Riboflavin

41
Q

What would you clean a blood spillage with?

A

Hypochlorite

42
Q

What causes black colonies on agar?

A
  • Blood agar- PORPHYROMAS GINGIVALIS – porphyrin pigments contain haem
43
Q

What is the main etiological factor in dental caries?

A

Oral bacteria associated with caries (S. mutans) use ATPase to pump H+ out of cells

44
Q

Which of these stabilizes calcium and phosphate?

A

Proline-rich proteins (PRPs) (statherin also does along with PRPs)

45
Q

Which of these is a virulence factor of S.mutans when sugar is low?

A

no clue tbh
Carbohydrate utilisation( Saccharolytic)? good at converting lots of different sugars to acids?
Expopolysaccarides

46
Q

What sweetener would you find in your drink?

A

Stevia

47
Q

Which of these is a pathogenic factor of N. gonorrhoea

A

Pilli - Genetic exchange (conjugation)

48
Q

After adding calcium hydroxyapatite to water at pH 7 and forming an equilibrium which is true?

A

hydroxyapatite will dissolve until an equilibrium is reached

49
Q

Which of these factors is a pathogenicity factor for S.mutans in acidic environment?

A
  • Reduced permeability of cell membrane to H+.
  • Induction of H+-translocating ATPase (expels protons from cells).
  • Induction of alkali production systems (arginine deiminase or urease).
  • Induction of stress proteins that protect enzymes and nucleic acids from denaturation
50
Q

Which of these is true about LPS?

A

Some things in my notes about LPS
• Endotoxin
• Found only in Gram-negative bacteria
• Present in the outer layer of the outer membrane
• Released during growth or when bacteria die and lyse
• Causes fever, septic shock and local inflammation

51
Q

What is Chlorhexadine?

A
  • Bisbiguanide (cationic – attracted to –ve charge)
  • Disrupts cell membrane phospholipids
  • High substantivity* (up to 12 hours)- relates to the persistence of its antimicrobial activity of the surface.
52
Q

What is a product that causes halitosis post hydrolysis?

A

Amino acids

53
Q

Which of these is not killed buy autoclave?

A

Prions

Extra Stage 2 (14 decks)

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