Methods Of Studying Cells

Question 1

What is the difference between magnification and resolution?

Answer 1

Magnification: is the increase in the apparent size of the object-the extent to which it has been enlarged
Resolution: is the ability to distinguish between 2 points that are closer together

Question 2

How does a light microscope work?

Answer 2

Eyepiece lens:
Magnifies and focuses the image

Objective lens:
Collects light passing through the specimen and produces and magnified image

Condenser lens: focuses light onto the specimen

Question 3

Advantages and disadvantages of TEM and SEM

Answer 3

+ TEM: give high resolution images and shows small objects
-TEM: can only be used on thin specimens and only dead

+SEM: can be used on thick specimens and 3D
-SEM: lower resolution than TEM. Is also only dead specimens

Question 4

What is an artifact?

Answer 4

Something that is seen under a microscope that isn’t part of a specimen- can be dust, air bubble, fingerprints- common in electron microscopes because they need a lot of preparation
To overcome this problem by repeatedly preparing specimens in different ways