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Microbiology > Methods Of Sterilization > Flashcards

Methods Of Sterilization Flashcards

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Biology 101 flashcards
1
Q

Sterilization

A

Sterilization is defined as the process by which an article surface or medium is freed of all living microorganism either in the vegetative or spore state

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2
Q

Disinfection

A

Disinfection is the destruction of all pathogenic organism

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3
Q

Asepsis

A

Asepsis is a state of complete absence of viable pathogenic microorganisms in any environment

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4
Q

Antiseptic

A

Antiseptic are the agents that can be safely applied on the skin or mucous Memon to prevent infection by inhibiting the growth of bacteria

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5
Q

Bactiricidal agents

A

That can kill bacteria

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6
Q

Bacteriostatic agent

A

Bacterio static agents prevent the multiplication of bacteria which may however remain alive. A chemical which is bactericidal at a particular concentration may become bacterio static at higher dilution

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7
Q

Sterling agents

A

Physical agents are dry heat, moist heat, filtration, radiation
Chemical agents are alcohol, aldehyde, dyes, hydrogen peroxide, hypochlorus acid halogen, phenol, surface acting agent metallic salt.

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8
Q

Dry heat

A

Flaming- inoculating loops, wire, forceps
Incineration - biomedical waste
Hot air oven - 160c for two hours to sterilize glassware, forceps, scissors, syringe
150 for opthalmic

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9
Q

Moist heat

A

BELOW 100C
Pasteurisation of milk
Inspissation
AT 100C
Boiling
Steam at atmospheric pressure
Tyndalisation
Steam under pressure

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10
Q

Pasteurisation temp

A

Flash method 72c for 15-20sec
Holder method 63c for 30 mins

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11
Q

Tyndalisation

A

For media containing sugar and gelatin
100c for 20 mins x 3days

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12
Q

Tyndalisation

A

For media containing sugar and gelatin
100c for 20 mins x 3days

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13
Q

Autoclave

A

Temp 108c to 147c
For linen, instrument, laboratory waree, media

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14
Q

Type of filters

A
  1. Candle filters - unglazed ceramic eg chamberland and doultan
    Diatomaceous earth eg Berkefeld and Mandler
  2. Sinister glass
    3 membrane
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15
Q

Properties of ideal disinfectant

A
  1. Have white spectrum of activity and be effective against all microorganisms
  2. Be active in the presence of organic matter
    3.v be effective in acid as well as alkaline media
  3. Have speedy action
  4. Has high penetrating power
  5. Be stable
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16
Q

Factors that affect the potency of a disinfectant

A
  1. Concentration of substance
  2. Contact period
  3. Ph of medium
  4. Temperature
  5. Nature of the organism
  6. Presence of extraneous material
17
Q

Mode of action of chemical agent

A
  1. By protein coagulation
  2. By disruption of the cell membrane resulting in exposure damage or loss of content
  3. Bye removal sulphydryl group essential for the functioning of the enzyme
  4. By substrate competition
18
Q

Alcohol

A

ETHYL - 60-90% conc
For denaturing bacterial proteins, no action on spores
METHYl - for fungal spores and cleaning cabinets and incubators

19
Q

Aldehydes

A

1.FORMALDEHYDE - active against the amino group in the protein molecule it is used to preserve anatomical specimen. It is used for sterilizing instrument heat sensitive catheter and for fumigating wards isolation room and laboratory
2. GLUTARALDEHYDE - it is effective against tubercular bacilli fungi viruses it has no delete areas effect on cement or lenses of instrument
3.ORTHOPTHALALDHYDEHDE- IT IS USED TO CLEAN ENDOSCOPE BETWEEN PATIENT AS IT IS QUICK EFFECTIVE AND SAFE
4.PERACETIC ACID - common antibiotic assistant bacteria
5. HYPOCHLOROUS ACID- biofilms

20
Q

Examples of aniline dye

A

Brilliant green
Malachite green
Crystal violet
Acts against tubercular bacilli

21
Q

Iodine

A

It is a bactericidal with moderate action against spores
Aqueous and alcoholic solution has been widely used as skin disinfectant

22
Q

Iodine

A

It is a bactericidal with moderate action against spores
Aqueous and alcoholic solution has been widely used as skin disinfectant

23
Q

Phenols

A

Phenols- cresol and Lysol
Chlorhexidine for wound dressing

24
Q

Ethylene oxide

A

Heart lung machine, respirators, suture material, dental equipments

25
Q

Radiation

A

Ionising - xray, gamma ray, cosmic ray
For plastics, syringe, swab, oil. Grease fabric

Non ionising
Infrared
Uv
For mass prepacked syringes, catheter, laboratory cabinets

26
Q

Spaulding’s classification

A

CRITICAL ITEMS -
High risk infection eg surgical instruments, cardiac catheter, implants
Method- autoclaving or hydrogen peroxide gas
SEMI CRITICAL- comes in contact with mucus membrane example endoscope and bronchoscopes
Method - glutaraldehyde
Alcohol

NON CRITICAL ITEMS
Low level disinfectant

Microbiology (6 decks)

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