Methods in Biochemistry Flashcards
1
Q
Spectrophotometry
A
inexpensive technique to measure light absorption/the amount of chemicals in a solution
2
Q
dye used in spectrophotometry for proteins
A
coomassie blue
3
Q
colour change in acidic conditions for coomassie blue
A
Brown –> blue
4
Q
qRT-PCR
A
- real time quantitive reverse transcription PCR
- that enables reliable detection and measurement of products generated during each cycle of PCR process
5
Q
measuring specific proteins via antibodies
A
6
Q
Western blot method
A
- detect “specific” protein in a tissue homogenate or protein extract
- homogenization = protein extraction
7
Q
Electrophoresis
A
used to help separate protein size for western blot method
8
Q
ELISA
A
- Enzyme Linked Immunosorbent Assay
- measuring specific proteins via antibodies
9
Q
PCR
A
- polymerase chain reaction
- a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail.
