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Method of Identifying Microorganism Flashcards

1
Q

Study organisms in their living or natural state
Demonstrates motility

A

Direct Method

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2
Q

Types of Direct Method

A

Wet mount method
Hanging Drop method

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3
Q

Parts of Hanging drop method

A

Depreession (well) slide
Cover slip
Petroleum Jelly (seal)
Drop of liquid with specimen

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4
Q

Examine organisms in their fixed or stained
state

A

Indirect Method

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5
Q

Types of Indirect Method

A

Smear preparation
Staining method

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6
Q

Specimen for smear preparation

A

Solid specimen
Liquid specimen

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7
Q

What to do with liquid specimen in smear preparation

A

air dry
heat fix

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8
Q

Attach or secure microorganisms on the glass slide
Kill the microorganism

A

Heat Fix

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9
Q

artificial coloring of the smear or microorganism
with dyes.

A

Staining Method

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10
Q

Make organism more visible
For identification and classification of
microorganisms
To emphasize special bacterial structure

A

Staining

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11
Q

2 types of staining

A

Simple staining
Differential staining

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12
Q

Utilizes only one dye or stain

A

Simple staining

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13
Q

Employs 2 dyes

A

Differential staining

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14
Q

Stains in differential staining

A

Gram Stain
Acid Fast Stain

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15
Q

Components of Gram Stain

A

Crystal Violet
Grams Iodine
Acid Alcohol
Safranin

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16
Q

Color of Gram Negative

A

Pink or Red

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17
Q

Color of Gram positive

A

Purple or Blue

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18
Q

All cocci are gram (+) except?

A

Neisseria
Branhamella
Veilonella
Moraxella

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19
Q

All Bacilli are gram (-) except?

A

Bacillus
Lactobacillus
Listeria
Actinomyces
Corynobacterium
Clostridium
Mycobacterium
Erysipelothrix
Nocardia spp

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20
Q

Theories of Gram Staining

A

Mg RNA
Kaplan-Kaplann
Stearn-Stearn

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21
Q

❖Gram (+) organism’s cell wall contains Mg RNA
❖ Gram (-) organisms cell wall do not contain Mg RNA

A

Mg RNA

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22
Q

❖ Gram (+) organisms are less permeable to decolorizer
❖ Gram (-) organisms easily decolorized by alcohol

A

Kaplan- Kaplan

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23
Q

❖ Gram (+) organisms have lower isoelectric point making them more
acidic
❖ Gram (-) organisms have higher isoelectric point making them more
basic

A

Stearn- Stearn

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24
Q

Gram stain cannot stain?

A

Mycobacteria
Mycoplasma
Rickettsiae
Chlamydia

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25
Q

Components of Acid Fast Stain

A

Carbol Fuchsin
Acid Alcohol
Methylene Blue

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26
Q

2 methods of Acid Fast stain

A

Zhiel-Neelsen Method
Kinyoun Method

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27
Q

Acid fast positive color

A

Red

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28
Q

Acid Fast negative

A

Blue

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29
Q

active detergent or surfactant in acid fast staining

A

Tergitol

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30
Q

artificially prepared material that provides the
nutritional and environmental requirements for
bacterial growth

A

Culture media

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31
Q

Basic Ingredients of culture media

A

Beef Extract
Peptones
Salts
Water
Agar

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32
Q

Types of Culture media state

A

Solid
Liquid
Semi-solid

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33
Q

With solidifying agent. It provides firm surface

A

Solid media

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34
Q

No solidifying agent. Remains in liquid form

A

Liquid media

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35
Q

Consistency of solid and liquid. Gelatinous consistensy. To demonstrate motility

A

Semi-Solid media

36
Q

how many percent of agar in solid media

37
Q

How many percent of agar in semi-solid media

38
Q

contains both a solid part and a liquid part in a single bottle

39
Q

Composition of culture media

A

Synthetic/Defined
Non-Synthetic/Undefined

40
Q

Method of Dispensing Culture media

A

Plated
Tubed

41
Q

Exact composition of the media is known

A

Synthetic/Defined

42
Q

Exact composition of the media is unknown/ Raw material

A

Non-Synthetic/Undefined

43
Q

Agar temperature at room temp

44
Q

Agar temperature at incubation

45
Q

Agar temperature at boiling

46
Q

Invented Gram Stain

A

Hans Christian Gram

47
Q

Thick; Multilayer

48
Q

Thinner; Single layer

49
Q

Bacteria with darting motiliity

A

Campylobacteria

50
Q

Bacteria with rapid darting/shooting star

A

Vibrio cholerae

51
Q

Bacteria with gliding motility

A

Capnocytophagia

52
Q

Bacteria with swaming motility

53
Q

Bacteria with tumbling motility

54
Q

Bacteria with twitching motility

55
Q

Emulsify is also known as

A

Round Coiled Type

56
Q

pH change that carries no electrical charge

A

isoelectric

57
Q

allows passages of liquid or gas

58
Q

Zhiel-Neelsen method
1. Stains
2. Color of AF and NAF

A

Carbol Fuchsin
Steam
Acid Alcohol
Methylene Blue

RED
RED
RED
RED

RED
RED
COLORLESS
BLUE

59
Q

Kinyoun method
1. Stains
2. Color of AF and NAF

A

Carbol Fuchsin
Phenol (10%)
Acid Alcohol
Methylene Blue

RED
RED
RED
RED

RED
RED
COLORLESS
BLUE

60
Q

General bacterial with Mycolic Acid

A

Mycobacterium
Nocardia
Rhodococcus
Gordonia
Tsukamurella
Corynebacterium

61
Q

Partially digested proteins, carbohydrates in the medium

62
Q

Maintain osmotic pressure in the medium

63
Q

Maintains the pH of the medium

64
Q

Cannot be degraded by microorganisms

65
Q

Process of introducing microorganism in the
culture medium

A

INOCULATION

66
Q

Sample of microorganism to be inoculated

67
Q

Prevention of microbial contamination

67
Q

the state of being free from disease- causing
contaminants

68
Q

using flame ( inoculating
loop/ needle)

A

Sterilization

69
Q

passing the test tube
mouth/ petri dish over the
flame

A

Sterilization

70
Q

Streaking pattern

A

Quadrant Streak
Continuous Streak
Radiant Streak

71
Q

maintain environmental condition

A

INCUBATION

72
Q

hours incubation period

73
Q

Optimum temperature of incubation

74
Q

Humidity of Incubation

A

70% or greater
No drying or desiccation

75
Q

O2 and CO2 Requirements

A

Anaerobic incubation

76
Q

Jar used in anaerobiosis

A

Gaspak Jar

77
Q

Ground meat media overlaid or sealed with petrolatum to prevent the
entry of air

A

Cooked Meat

78
Q

Acts as reducing agents

A

Mixture of Pyrogallol, KOH, and Sodium Bicarbonate

79
Q

Aids in the initiation and growth of small inoculum and anaerobes by
impeding the diffusion of oxygen into the medium.

A

Thioglycollate media

80
Q

Growth of microorganisms in a culture media

81
Q

Types of Culture

A

Pure Culture
Mixed Culture

82
Q

Macroscopic growth seen in a culture media

83
Q

Culture that is less contaminated

A

Pure culture

84
Q

Culture that is erroneous or no identification

A

Mixed Culture

85
Q

hydrogen catalytically combines with oxygen in the air to form water

A

ANAEROBIC JARS

86
Q

Techniques of Anaerobic Jars

A

Evacuation replacement technique
Disposable gas generator envelope

Bacte Lec (4 decks)

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