Classification of Culture Media Flashcards
Growth of microorganisms in a culture medium.
CULTURES
Composed of mixture of nutrients essential for microbial growth
CULTURE MEDIUM
These are routine in the laboratory and without additional supplements.
BASIC/PRIMARY/ALL-PURPOSE MEDIA
Supports the growth of most non-fastidious bacteria
BASIC/PRIMARY/ALL-PURPOSE MEDIA
It contains all bacis ingredients and supports the growth of unwanted organism
BASIC/PRIMARY/ALL-PURPOSE MEDIA
Example of BASIC/PRIMARY/ALL-PURPOSE MEDIA
Nutrient Agar
Nutrient Broth
Tryptic Soy Agar
Contain specific nutrients required for the growth of particular bacterial pathogens that may be present alone or with other bacterial species in a patient specimen
ENRICHMENT MEDIA
Designed to temporarily inhibit the growth of unwanted organisms but nourish and support an organisms of interest
ENRICHMENT MEDIA
Contain one or more agents that are inhibitory to all organisms except those “selected” by the specific growth condition or chemical.
SELECTIVE MEDIA
Examples of SELECTIVE MEDIA
(Discussed)
Mannitol Salt Agar
Brilliant Green Agar
(Extra)
Hektoen Enteric Agar
MacConkey Agar
Thayer-Martin Agar
Agar designed for Gram (+) only
Mannitol Salt Agar
Agar designed for Salmonella Typhi
Brilliant Green Afar
Media with additional supplements such as blood, vitamins, and yeast extract, which are necessary for the growth of fastidious organisms.
ENRICHED MEDIA
Culture media used to grow microorganisms of a wide variety
-It containts nutritive supplements
ENRICHED MEDIA
Example of ENRICHED MEDIA
Blood Agar Medium
Chocolate Agar Plate
Loefflers Agar media
Agar for Gram (-) bile salts crystal violet
MacConkey Agar
Employ some factor (or factors) that allow colonies of one bacterial species or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing on the same agar plate.
DIFFERENTIAL MEDIA
Example of DIFFERENTIAL MEDIA
MacConkey agar
Blood Agar plate
Used to isolate bacteria with specific growth requirements
SPECIAL MEDIA
Example of SPECIAL MEDIA
Lowenstein-Jensen Medium
TCBS
Blood Agar Types of Hemolysis
Beta Hemolysis (Broad)
Alpha Hemolysis (Dark broad)
Gamma Hemolysis (Slightly Visible)
