Metabolism II and III

Question 1

Enzyme catlyst activity is dependant on

Answer 1

ph, temperature

Question 2

Metal ion catalysis may be achieved through the following mechanisms:

Answer 2

mediating redox reaction by transiently changing redox state

Question 2

What is the velocity of a first order reaction at 300K when the reactant concentration is 1 M and the rate of constant is 2 sec

Answer 2

2/1 = 2 M/sec

Question 2

During Oxidative phosphorylation, an electrochemical gradient is formed across the

Answer 2

inner mitochandiral membrane

Question 2

Km is equal to

Answer 2

the [S] when the enzyme-catalyzed reaction rate is half-maximal

Question 2

During glycolysis, the steps between glucose and formation of the Glyceraldehyde 3-phosphate
(3) consumes

Answer 2

2 ATP molecules

Question 3

In glycolysis ATP synthesis is catalyzed by

Answer 3

pyruvate kinase

Question 4

What are the most important inhibitors in the electron transport chain (ETC)

Answer 4

Amytal, Rotenone, Antimycin A, Cyanides.

Question 5

Phosphofructokinase is allosterically —– by high contrations of—–

Answer 5

inhibited; ATP

Question 6

Glycolysis takes place in the

Answer 6

cytosol

Question 7

What is the net reaction equation for glycolysis

Answer 7

Glucose + 2ADP + 2Pi +2NAD+ → 2 Pyr + 2ATP + 2NADH + 4H+ + 2H2O

Question 8

What is the first stage of glycolysis

Answer 8

investment

Question 9

What are the 3 control points in glycolysis?

Answer 9

Hexokinase
o Phosphofructokinase (step 3)
o Pyruvate kinase (step 10

Question 10

The investment stage of glycolysis spends and for what

Answer 10

2 ATP for activation

Question 11

Are the 3 enzymatic control points of glycolysis, reversible or irreversible?

Answer 11

Irreversible

Question 12

Hekinoses is inhibited by its

Answer 12

product

Question 13

Phosphofructokinase is inhibitied by

Answer 13

ATP and citrate

Question 14

Phosphofructokinase is activated by

Answer 14

AMP and fructose 2,6-bisphosphate,

Question 15

Pyruvate kinase is activated by

Answer 15

AMP and fructose-1,6-bisphosphate,

Question 16

Pyruvate Kinase is inhibited by

Answer 16

ATP and acteyl-CoA

Question 17

Under aerobic conditions pyruvate gets processed through the

Answer 17

processed through the TCA cycle (or citric acid cycle or Krebs cycle)

Question 18

Under anareobic conditions, pyruvate goes through

Answer 18

Lactic fermentation and alcohol fermantation

Question 19

Alcohol fermentation recycles. what compound

Answer 19

NADH

Question 20

TCA cycle takes place in the

Answer 20

mitochondrial matrix

Question 21

Which steps in the TCA cycle are oxidative reactions

Answer 21

step 3 and step 4

Question 22

During steps 7 and 10, phosphoglycerate kinase (PGK) and pyruvate kinase (PK) synthesize

Answer 22

ATP via substrate-level phosphorylation

Question 23

Oxidative phosphorylation takes place at the

Answer 23

electron transport chain in the mitochondria

Question 24

Oxidative phosphorylation is

Answer 24

cellular process that harnesses the reduction of oxygen to generate phosphate bonds in the form of ATP (consumes oxygen)

Question 25

In substrate-level phosphorylation, the substrate reacts to form

Answer 25

a product containing high energy bonds

Question 26

What is substrate-level phosphorylation?

Answer 26

Phosphorylation of ADP into ATP at the expense of substrate (oxygen-independent

Question 27

Stage 2 of glycolysis is called---- and yields

Answer 27

payoff; 4 ATP

Question 28

least oxidative to most oxidative state of carbon

Answer 28

alkane, alcohol, aldehyde, carboxylic acid, carbon dioxide

Question 29

uncompetitive inhibition

Answer 29

binds only to the ES not free enzyme

Question 30

uncompetitve inhibition