Metabolism and Enzymes Flashcards

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1
Q

what is a chemical reaction

A

a chemical reaction is a process that a substrate undergo chemical change in which one or more new substances are formed

like glucose + oxygen –> carbon dioxide + water + (energy)

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2
Q

collision theory

A

matters exist as particles and particles are constantly moving

in order for a chemical reaction to occur, particles of reactant must first collide with each other

only a small proportion of collision can lead to chemical reactions. this kind of collision is called effective collision

energy carried by the reactant particles and the orientation of the particles in the collision determines whether an effective collision can occur

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3
Q

Metabolism

A

Metabolism refers to all the chemical reactions which keep the cell alive

The molecules involved in metabolism are called metabolites

the overall speed of the metabolism of an organism is called metabolic rate

metabolism can be divided into catabolism and anabolism

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4
Q

catabolism

A

breaking down of complex molecules into simple molecules

energy is released (a decrease in energy level)

breaking down of glucose into water and carbon dioxide during respiration

breaking down of excess amino acids into urea in liver

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5
Q

anabolism

A

building up of complex molecules from simple molecules

energy is absorbed (an increase in energy level)

building up of glucose from water and carbon dioxide during photosynthesis

building up of cell wall from glucose

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6
Q

roles of enzyme in metabolism

A

enzyme speeds up chemical reactions in organisms

enzyme increases the metabolic rate by lowering the activation energy so that metabolism in living organisms occurs rapidly enough to sustain life, even at the relatively low temperatures ( the body temperatures of human is just 37 degrees)

without enzyme, metabolism would be too slow to sustain life or even would not occur at all

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7
Q

properties of enzymes (1)

A

all enzymes are protein

since all enzymes are protein, any properties of protein are also properties of enzymes

for example, protein structure is easily affected by temperature and pH. Thus, enzyme activity is also affected by temperature and pH

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8
Q

properties of enzyme (2)

A

enzyme action is highly specific

usually an enzyme can only catalyse one type of reaction

The shapes of enzymes and substrate molecules are complementary

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9
Q

properties of enzymes (3)

A

enzymes are biological catalysts

enzymes lower the energy barrier/activation energy required for chemical reactions to take place

Enzymes speed up metabolism in living organisms

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10
Q

properties of enzymes (4)

A

enzymes are reusable and efficient in action

enzymes temporarily combine with substrates

they remain unchanged after the reaction and can catalyse the reaction again

only a small amount of enzyme is enough

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11
Q

action of enzyme

A

each enzyme has an active site where the substrate and enzyme can combine

a reaction begins when the active site of an enzyme combines with the suitable substrate to form a temporary enzyme-substrate complex

the activation energy of the chemical reaction is reduced by forming this complex

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12
Q

catabolic reaction

A

the active site of an enzyme combines with the substrate

the enzyme then speeds up the breaking down of the substrate into product

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13
Q

anabolic reaction

A

the active site of an enzyme combines with two or more substrates

the enzyme then speeds up the building up of substrates into product

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14
Q

lock and key hypothesis

A

the specific action of enzyme can be explained by the lock-and-key hypothesis

a key of a specific shape opens only one lock

the shape of the substrate is complementary to the active site of the enzyme molecules so that the substrate can insert into the active site of the enzyme

each enzyme acts on only one or a few substrate that fits into its active site, and it therefore catalyzes only one kind of reaction

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15
Q

at low temperature like 10 degrees

A

rate of enzymatic reaction
low

enzymes are inactive in low temperatures

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16
Q

increasing temperature like 10 to 39 degrees

A

rate of enzymatic reaction
increase

the inactivation of enzymes by low temperature is reversible

as temperature increases, the kinetic energy of the substrates of enzymes increase

the substrate and enzyme vibrate faster and lead to increased collision between enzymes and substrates

more enzyme-substrate complexes can be formed

17
Q

optimum temperature like 40 degrees

A

rate of enzymatic reaction
highest

enzymes work best at optimum temperature

different enzymes have different optimum temperatures

18
Q

high temperatures like over 40 degrees

A

rate of enzymatic reaction
decrease

as temperature increases beyond the optimum temperature, the active sites of the enzymes are destroyed.

the enzymes are said to be denatured

the denaturation of enzymes is irreversible (the activities of enzymes cannot be resumed even after cooling down the enzymes which have been exposed to high temperature previously)

19
Q

how are enzymes affected by pH

A

enzymes work within a narrow range of pH

enzymes work best at optimum pH

pH other than the optimum pH changes the shape of the active sites of enzymes

the substrates can no longer fit into the active sites to form the enzyme-substrate complexes

the rate of enzymatic reaction thus decreases

enzymes are said to be denatured by extreme pH

20
Q

what is hydrogen peroxide

A

it is continuously produced as a by-product in the metabolism of living things

it is toxic and would cause harm to organisms if it is allowed to accumulate at a high level

21
Q

catalase

A

it is found in almost any kind of living tissue. it is abundant in liver tissue as liver is for breaking down toxic substances

it breaks down hydrogen peroxide into oxygen and water

the production of oxygen can be used to indicate the breaking down action of catalase on hydrogen peroxide

22
Q

how is the time taken for the reaction of starch to maltose

A

the time taken for the appearance of all maltose

the time taken for the disappearance of all starch (easier)