Metabolism Flashcards
Metabolism
Kreb’s cycle
in notes
organism : streptococcus / lactobacillus
end product : ?
lactic acid: breaks enamel of teeth . in vaginal and oral flora
organism : saccharomyces.
end product :?
ethanol and co2
Organism: propionibacterium
end product :
propionic acid, acetic acid, CO2, H2
organism clostridium.
end product:
butyric acid, butanol, acetone, isopropyl alcohol, CO2
Butyric acid: burn victims
organism: escherichia, salmonella
end product :?
ethanol, lactic acid, acetic acid, co2, H2
organism : enterobactium
ethanol, lactic acid, formic acid`
explain the source of each ATP from cell respiration
Glycolysis:
2 ATP: glucose to pyruvate
6 ATP from 2 NADH.
Prep stage :
acetyl coa creates 2NADH so 6ATP.
Krebs
succinyl coa to succinic acid:2GTP
6NADH will make 18ATP
2FADH will make 4ATP.
explain catalase test
Hydrogen peroxide reduced to Oxygen Left (+) Staph species Right (--) Streptococcus species
manitol agar test
Acid production (yellow) (+) Pink (--) Staph aureus (+) Staph epidermidis (-)
Coagulase Test
Coagulase Test Staph aureus (+) Staph epidermidis (-)
what type of amino acids are made from each ?
PEP—
Pyruvate—
Oxaloacetate—
PEP—aromatic amino acids
Pyruvate—aliphatic amino acids
Oxaloacetate—aspartate amino acids
Biosynthesis In Fermentation
Krebs provides intermediates for?
amino acids & hemin synthesis
at what kcal in the etc can an ATP be generated
7
at what points are ATP generated in the ETC
After NADH2 when kcal id 12.8
after cytchrome b when kcal is 8.8
after cyt. a/a3: kcal 23.5
Name types of bacterial proteins (10)?
Permeases Receptors Proteoglygans Bacteriocins B-lactamases Secretory IgA proteases Hemolysins Leucocidins Metabolic enzymes Molecular chaperons