Metabolism Flashcards
Metabolism
Kreb’s cycle
in notes
organism : streptococcus / lactobacillus
end product : ?
lactic acid: breaks enamel of teeth . in vaginal and oral flora
organism : saccharomyces.
end product :?
ethanol and co2
Organism: propionibacterium
end product :
propionic acid, acetic acid, CO2, H2
organism clostridium.
end product:
butyric acid, butanol, acetone, isopropyl alcohol, CO2
Butyric acid: burn victims
organism: escherichia, salmonella
end product :?
ethanol, lactic acid, acetic acid, co2, H2
organism : enterobactium
ethanol, lactic acid, formic acid`
explain the source of each ATP from cell respiration
Glycolysis:
2 ATP: glucose to pyruvate
6 ATP from 2 NADH.
Prep stage :
acetyl coa creates 2NADH so 6ATP.
Krebs
succinyl coa to succinic acid:2GTP
6NADH will make 18ATP
2FADH will make 4ATP.
explain catalase test
Hydrogen peroxide reduced to Oxygen Left (+) Staph species Right (--) Streptococcus species
manitol agar test
Acid production (yellow) (+) Pink (--) Staph aureus (+) Staph epidermidis (-)
Coagulase Test
Coagulase Test Staph aureus (+) Staph epidermidis (-)
what type of amino acids are made from each ?
PEP—
Pyruvate—
Oxaloacetate—
PEP—aromatic amino acids
Pyruvate—aliphatic amino acids
Oxaloacetate—aspartate amino acids
Biosynthesis In Fermentation
Krebs provides intermediates for?
amino acids & hemin synthesis
at what kcal in the etc can an ATP be generated
7
at what points are ATP generated in the ETC
After NADH2 when kcal id 12.8
after cytchrome b when kcal is 8.8
after cyt. a/a3: kcal 23.5
Name types of bacterial proteins (10)?
Permeases Receptors Proteoglygans Bacteriocins B-lactamases Secretory IgA proteases Hemolysins Leucocidins Metabolic enzymes Molecular chaperons
B-lactamases
degrade anitbiotics
secretory IgA protease
dimeric. on mucosa surface. prevent colonization of exogenous bacteria
How is the reductive patheay possible in the biosynthesis of fermentation
Reductive is possible because NAD-linked succinate dehydrogenase is replaced by fumarate reductase
what molecules are responsible for pyrimidine synthesis and what triphospates are produced ?
aspartic acid.
carbamyl phosphate .
orotic acid.
b, UTP, TTP AND CTP
what components are responsible for purine synthesis and what triphospates are produced ?
Ribose-5-phosphate. Glycine. Serine. IMP
and
ATP, GTP
nucleic acid inhib?
rifampin and quinolones
rifampin
Rifampin
Inhibits RNA synthesis by binding to DNA-dependent RNA polymerase
Treats TB
quinolones
Quinolones Ciprofloxacin Inhibit gyrase or topoisomerases Prevent DNA supercoiling Treats various bacterial infections UTIs,Pneumonia
