Membranes - fill in the blanks

Question 1

Answer 1

1. Pyruvate
2. Citrate
3. Mitochondrion
4. Oxaloacetate
5. citrate
6. Synthesis of aminoacids, other metabolites
7. Acetylation of cytoplasmic proteins
8. Acetyl-CoA
9. Cytosol
10. Nucleus
11. Histone acetylation
12. Long-chain fatty acid biosynthesis
13. Pyruvate
14. Acetate
15. Fatty Acids
16. Plastid
17. Fatty acids
18. Malate
19. Peroxisome
20. Sucrose
21. ACL

Question 2

Answer 2

1. Na+ binds to pump
2. Pump phosphorylates itself
3. phosphorylation triggers conformational change, Na+ ejected
4. K+ binds
5. pump dephosphorylated
6. pump returns to original conformation, K+ ejected

Question 3

Answer 3

1. SRP binds to ribosme/signal sequence
2. GDP phosphorylated to GTP
3. SRP binds to receptor
4. Protein inserted into translocon
5. GTP hydrolysis leads to release of SRP

Question 4

Answer 4

1. Synthesis of cardiolipin
2. Fatty acid elongation and lipid synthesis
3. Fatty acid biosynthesis in eukaryotes
4. Malonyl-CoA synthesis and fatty acid synthesis in prokaryotes
5. Synthesis of cardiolipin
6. Malonyl-CoA synthesis for fatty acid elongation in eukaryotes
7. fatty acid elongation and lipid synthesis

Question 5

Answer 5

1. H+ binds from outside at Glu 269
2. Protonated form binds lactose
3. Conformation change
4. release lactose
5. release H+
6. Conformation change reset

Question 6

Answer 6

1. Single alpha helix
2. multiple alpha helices
3. rolled up beta sheet (beta barrel)
4. amphilic alpha helix anchor
5. covelently bound lipid chain
6. oligosaccharide linker - GPI anchor
7. membrane associated protein (peripheral)
8. membrane associated protein (peripheral)

Question 7

Answer 7

1. Donor membrane
2. Cargo
3. Loading
4. budding
5. movement
6. tethering/docking
7. Fusion
8. Acceptor membrane
9. Cargo