membrane proteins Flashcards
are membranes flexible
yes
are membranes self sealing
yes
when does shape change of membrane occur
growth, division, endo/exocytosis
are membranes thick or thin
thin - essentially 2D
why is it good that membranes are thin
they enhance reaction rates
are membranes permeably
selectively permeable
can polar molecules pass through the membrane
they have a limited ability to pass through
what drives the formation of the membrane
hydrophobic effect
where are a majority of the glycolipids in the membrane
extracellular lumen
are glycolipids more on the cytosolic or lumen side
lumen
how do individual lipids orient in the bilayer
based on distribution of hydrophobic and hydrophilic character
what/were is lateral diffusion
within a leaflet
how fast is lateral diffusion + why
very rapid because interactions are non covalent and there is little barrier to motion
how fast is transverse diffusino
slow
what are the 3 stages of increasing temperatures in bilayers
gel phase –> liquid ordered state –> liquid disordered state
what is another name for the liquid ordered state
the liquid crystal state
how do they maintain fluidity in membranes
by altering membrane lipids (homeoviscous adaptation)
what is the protein-lipid composition myelin sheath and why
more fatty and glycolipids (lower polarity) because its an insulator for nerve signals
what is the protein-lipid composition inner mitochondrial membranes and why
high in protein cause of energy production OXPHOS, ETC
where are the carbohydrate aspects of glycoproteins/ glycolipids (where in the plasma membrane)
facing exterior to cell at the plasma membrane
where does synthesis of lipids carry out in eukaryotes
integral membrane proteins in ER in eukaryotes
where does synthesis of lipids carry out in prokaryotes
integral membrane proteins in plasma membrane in eukaryotes
what can happen with an appearance of specific lipids in other leaflets trigger
+ example
it can trigger other events
appearance of phosphatidylserine in outer leaflets triggers cell for engulfment/degradation
what does appearance of phosphatidylserine in outer leaflets trigger
engulfment/degradation
are membranes symetrical
no
are lipids distributed symmetrically in the membrane
no
how are lipids distributed in the membrane
asymmetrically
how are proteins distributed in the membrane
they all have the same orientation/directionality in the membrane (like all the same proteins in the same membrane would be pointing in the same direction, not like one is up and another is down)
where are most choline containing lipids in erythrocyte membranes
primarily in the outer leaflet
where are most sphingomyelin in erythrocyte membranes
primarily in the outer leaflet
where are most phosphatidylcholine lipids in erythrocyte membranes
primarily in the outer leaflet
which lipid should only be in the inner leaflet
phosphatidylserine primarily in the outer leaflet
what can changes in phospholipid distribution signal
signal or target cell for death
how fast is movement of lipids from one from one leaflet to another in bilayers
slow
how fast is movement of lipids from one from one leaflet to another in membranes
rapid
what do integral membrane proteins do for movement of lipids
help reduce the energy barrier for movement of lipids
what kind of proteins help reduce the energy barrier for movement of lipids
integral membrane proteins
what does lipid asymmetrical transport require
input of energy (active transport process)
what do flippases do
import lipids from outside the cell to inside the cell
what do floppases do
export lipids from interior of the cell to the exterior of the cell
what do scramblases do
passive transport to equalize concentrations between leaflets
is flippase active or passive (What kind)
primary active
is floppase active or passive (What kind)
primary active
is scramblases active or passive
passive transport
which flip or flop would deal with phosphatidylserine
flippase
which flip or flop would deal with phosphatidylethanolamine
flippase
which flip or flop would deal with phosphatidylcholine
floppase
which flip or flop would deal with phosphatidylsphingolipids
floppase
can proteins associated with the cytoskeleton diffuse laterally
maybe not
why can some proteins not be able to diffuse laterally
if theyre anchored/associated with the cytoskeleton
what are the 3 main types of membrane proteins
peripheral
lipid linked
integral
what are the integral membrane proteins
- lipid linked
- integral
are peripheral membrane proteins integral
no
are lipid linked membrane proteins integral
yes
how can you isolate/remove peripheral proteins
through moderate changes like pH and salt
how can glycosylated phosphoinositol linked proteins be removed from the membrane
through the action fo phospholipases (C or D)
are proteins or lipids more free to move in the membrane
lipids usually i think
how can you isolate/remove integral membrane proteins
using detergents or organic solvents to mimic the hydrophobic environment of the membrane
are integral or peripheral membrane proteins easier to isolate from the membrane
peripheral
how can you classify integral membrane proteins (2)
based on structural composition of transmembrane region
or
topology
what are 3 classifications on integral membrane proteins
whether the transmembrane region is
- single helices
- helical bundles
- beta barrels
what are 2 classifications for classifying membrane proteins based on topology
- how many times they pass the membrane
- where the N terminal is located
what is bitopic
when the proteins passes 1 time through the membrane
what is polytopic
when the protein passes multiple times through the membrane