Membrane Biosynthesis

Question 1

Core Oligosaccharide

Answer 1

Highly branched
14 sugar residues, includes a 3 glucose terminal branch

Synthesized by glycotransferases

Question 2

Glycosylation Sequence

Answer 2

N-x-S/T

Question 3

Mono-UB

Answer 3

Serves as a signal for membrane protein import into late endosomal intralumenal vesicles

Question 4

Poly-UB

Answer 4

Serves as a signal for ER protein degradation and for most other cellular proteins destined for normal turnover

Question 5

PERK

Answer 5

Protein Kinase RNA-linke ER Kinase

Question 6

ATF6

Answer 6

Activating Transcription Factor 6

Question 7

Protein sensors

Answer 7

Integral membrane proteins

Have lumenal stress sensing domains that bind to molecular chaperones

PERK, ATF6

Question 8

eIF2alpha

Answer 8

Cytosolic protein TF required for the initiation of protein synthesis

Involved in ribosome-mRNA binding