Golgi

Question 1

ERGIC

Answer 1

ER-Golgi Intermediate Compartment

Initial destination of COPII vesicles from the ERES

Vesicles can fuse to form larger vesicles and interconnected tubules (VTC)

Question 2

VTC

Answer 2

Vesicular Tubular Carriers

Move towards CGN via cytoskeleton

Question 3

GRASPS

Answer 3

Golgi Re-Assembly and Stacking Proteins

Serve as tethering proteins to link different Golgi subcompartments together

Question 4

Golgi Matrix Proteins

Answer 4

Determines shape of golgi (connects CGN, cisternae, TGN)

Connects Golgi to cytoskeleton

Question 5

Cisternal Progression/Maturation Model

Answer 5

Most favoured mofel

Golgi subcompartments are dynamic structures- composition of subcompartment changes while moving from cis to trans (CGN becomes cis becomes medial…etc)

Golgi resident proteins constantly being moved back to proper subcompartment

Question 6

GGA adaptor coat proteins

Answer 6

Golgi-localizing gamma-adoptin ear homology domain ARF-binding proteins

Cytoplasmic proteins with multiple protein-protein binding domains

Serves as linkers during clathrin coat vesicle assembly

Question 7

GammaGTP

Answer 7

Non-hydrolyzable analog of GTP

Dynamin ring polymerization continues (not hydrolysis), vesicle not cleaved

Question 8

MVB

Answer 8

Multivescular Body

Lysosomal cargo proteins are partitioned into a specific area (domain) in the late endosome that eventually fragments (detaches)

Question 9

ESCRT

Answer 9

Endosomal Sorting Complex Required for Transport

Mediates inward budding of MVB membrane